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01.12.2015 | Original Contribution

Impact of basal diet on dextran sodium sulphate (DSS)-induced colitis in rats

verfasst von: Ahlem Boussenna, Nicolas Goncalves-Mendes, Juliette Joubert-Zakeyh, Bruno Pereira, Didier Fraisse, Marie-Paule Vasson, Odile Texier, Catherine Felgines

Erschienen in: European Journal of Nutrition | Ausgabe 8/2015

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Abstract

Purpose

Dextran sodium sulphate (DSS)-induced colitis is a widely used model for inflammatory bowel disease. However, various factors including nutrition may affect the development of this colitis. This study aimed to compare and characterize the impact of purified and non-purified basal diets on the development of DSS-induced colitis in the rat.

Methods

Wistar rats were fed a non-purified or a semi-synthetic purified diet for 21 days. Colitis was then induced in half of the rats by administration of DSS in drinking water (4 % w/v) during the last 7 days of experimentation. At the end of the experimental period, colon sections were taken for histopathological examination, determination of various markers of inflammation (myeloperoxidase: MPO, cytokines) and oxidative stress (superoxide dismutase: SOD, catalase: CAT, glutathione peroxidase: GPx and glutathione reductase: GRed activities), and evaluation of the expression of various genes implicated in this disorder.

Results

DSS ingestion induced a more marked colitis in animals receiving the purified diet, as reflected by higher histological score and increased MPO activity. A significant decrease in SOD and CAT activities was also observed in rats fed the purified diet. Also, in these animals, administration of DSS induced a significant increase in interleukin (IL)-1α, IL-1β and IL-6. In addition, various genes implicated in inflammation were over-expressed after ingestion of DSS by rats fed the purified diet.

Conclusions

These results show that a purified diet promotes the onset of a more severe induced colitis than a non-purified one, highlighting the influence of basal diet in colitis development.

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Titel: Impact of basal diet on dextran sodium sulphate (DSS)-induced colitis in rats
verfasst von: Ahlem Boussenna
Nicolas Goncalves-Mendes
Juliette Joubert-Zakeyh
Bruno Pereira
Didier Fraisse
Marie-Paule Vasson
Odile Texier
Catherine Felgines
Publikationsdatum: 01.12.2015
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Nutrition / Ausgabe 8/2015
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-014-0800-2

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Abstract

Purpose

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