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01.10.2015 | Original Contribution

Increased body fat mass and tissue lipotoxicity associated with ovariectomy or high-fat diet differentially affects bone and skeletal muscle metabolism in rats

verfasst von: Camille Tagliaferri, Jérôme Salles, Jean-François Landrier, Christophe Giraudet, Véronique Patrac, Patrice Lebecque, Marie-Jeanne Davicco, Audrey Chanet, Corinne Pouyet, Amélie Dhaussy, Alain Huertas, Yves Boirie, Yohann Wittrant, Véronique Coxam, Stéphane Walrand

Erschienen in: European Journal of Nutrition | Ausgabe 7/2015

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Abstract

Purpose

The aim of this study was to evaluate and compare the musculoskeletal effects induced by ovariectomy-related fat mass deposition against the musculoskeletal effects caused by a high-fat diet.

Methods

A group of adult female rats was ovariectomized and fed a control diet. Two additional groups were sham-operated and fed a control or a high-fat diet for 19 weeks. Distal femur and serum bone parameters were measured to assess bone metabolism. Muscle protein metabolism, mitochondrial markers and triglyceride content were evaluated in tibialis anterior. Triglyceride content was evaluated in liver. Circulating inflammatory and metabolic markers were determined.

Results

The high-fat diet and ovariectomy led to similar increases in fat mass (+36.6–56.7 %; p < 0.05) but had different impacts on bone and muscle tissues and inflammatory markers. Consumption of the high-fat diet led to decreased bone formation (−38.4 %; p < 0.05), impaired muscle mitochondrial metabolism, muscle lipotoxicity and a 20.9 % increase in tibialis anterior protein synthesis rate (p < 0.05). Ovariectomy was associated with higher bone turnover as bone formation increased +72.7 % (p < 0.05) and bone resorption increased +76.4 % (p < 0.05), leading to bone loss, a 17.9 % decrease in muscle protein synthesis rate (p < 0.05) and liver lipotoxicity.

Conclusions

In female rats, high-fat diet and ovariectomy triggered similar gains in fat mass but had different impacts on bone and muscle metabolism. The ovariectomy-induced mechanisms affecting the musculoskeletal system are mainly caused by estrogen depletion, which surpasses the potential-independent effect of adiposity.

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Titel: Increased body fat mass and tissue lipotoxicity associated with ovariectomy or high-fat diet differentially affects bone and skeletal muscle metabolism in rats
verfasst von: Camille Tagliaferri
Jérôme Salles
Jean-François Landrier
Christophe Giraudet
Véronique Patrac
Patrice Lebecque
Marie-Jeanne Davicco
Audrey Chanet
Corinne Pouyet
Amélie Dhaussy
Alain Huertas
Yves Boirie
Yohann Wittrant
Véronique Coxam
Stéphane Walrand
Publikationsdatum: 01.10.2015
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Nutrition / Ausgabe 7/2015
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-014-0790-0

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Increased body fat mass and tissue lipotoxicity associated with ovariectomy or high-fat diet differentially affects bone and skeletal muscle metabolism in rats

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