Background
Results
INTS6/DICE1 mRNA expression is down-regulated in prostate cancer cells
Exogenous expression of DICE1 inhibits the clonogenic formation capacity of androgen-independent cell lines through an apoptosis-independent pathway
DICE1 expression induces G1 arrest in androgen-independent cell lines
Changes in the Wnt signaling pathway in response to DICE1
Gene Symbol | Description | GenBank | FC |
---|---|---|---|
Upregulated genesa | |||
AXIN1 | Axin 1 | NM_003502 | 2.14 |
CTBP2 | C-terminal binding protein 2 | NM_022802 | 2.14 |
CXXC4 | CXXC finger 4 | NM_025212 | 7.5 |
FZD7 | Frizzled homolog 7 (Drosophila) | NM_003507 | 4.0 |
JUN | Jun Oncogen | NM_002228 | 2.8 |
MYC | V-myc myelocytomatosis viral oncogen homolog (avian) | NM_002467 | 4.0 |
SLC9A3R1 | Solute carrier family 9 (sodium/hydrogen exchanger) | NM_004252 | 2.64 |
T | T, brachyury homolog (mouse) | NM_003181 | 2.3 |
TCF7L1 | Transcription factor 7-like 1 (T-cell specific, HMG-box) | NM_031283 | 3.24 |
TLE | Transcucin-like enhancer of split (E(sp1)homolog, Drosophila | NM_005077 | 2.14 |
WISP1 | WNT1 inducible signalling pathway protein | NM_003882 | 3.24 |
WNT3 | Wingless-type MMTV integration site family, member 3 | NM_030753 | 2.3 |
WNT5B | Wingless-type MMTV integration site family, member 5B | NM_032642 | 3.5 |
Downregulated genesb | |||
APC | Adenomatosis polyposis coli | NM_000038 | -2.5 |
BTRC | Beta-transducin repeat containing | NM_033637 | -2.7 |
CCND1 | Cyclin D1 | NM_053056 | -3.8 |
FBXW2 | F-box and WD repeat domain containing 2 | NM_012164 | -3.3 |
FZD6 | Frizzled homolog 6 (Drosophila) | NM_003506 | -2.5 |
SENP2 | SUMO1/sentrin/SMT3 specific peptidase 2 | NM_021627 | -2.3 |
TCF7 | Transcription factor 7 (T-cell specific, HMG-box) | NM_003202 | -3.6 |
Discussion
Conclusion
Methods
Cell Lines
RNA isolation and Northern Blot analysis
Colony formation assay
Western blot analysis
Cell cycle analysis
Reverse transcription polymerase chain reaction (RT-PCR)
Gene Symbol | Forward Primer 5' → 3' | Reverse Primer 5' → 3' | Amplicon size (bp) | Annealing Temp. (°C) |
---|---|---|---|---|
G3PDH | TGGTATCGTGGAAGGACTCA | ATGCCAGTGAGCTTCCCGTT | 188 | 58 |
DICE1 | TGCCCATCTTACTGTTCCTG | TCTTCGAAAGTGACCAGC | 169 | 58 |
CXXC4 | TGCAAGAGGCTCATCAACTG | TCATTTCCAAATGCCTTGAA | 204 | 60 |
CCND1 | AGGAACAGAAGTGCGAGGAG | GGCGGATTGGAAATGAACT | 394 | 60 |
TCF7L1 | ACGAGCTGATCCCCTTCC | TGACCTCGTGTCCTTGACTG | 400 | 60 |