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Inflammation Research
Erschienen in: Inflammation Research 9/2015

01.09.2015 | Original Research Paper

Characterization of microRNA expression profiling in peripheral blood lymphocytes in rats with experimental autoimmune uveitis

verfasst von: Dadong Guo, Jiao Li, Zhengfeng Liu, Kai Tang, Huixin Song, Hongsheng Bi

Erschienen in: Inflammation Research | Ausgabe 9/2015

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Abstract

Objective and design

We aimed to investigate the alterations of microRNA (miRNA) genomics in peripheral blood lymphocytes in experimental autoimmune uveitis (EAU) rats versus control samples.

Materials/methods

Six Lewis rats received interphotoreceptor retinoid-binding protein (IRBP) emulsion to induce EAU. On day 12, peripheral blood lymphocytes were isolated, and total RNAs were extracted. Using microarray analysis, we analyzed the aberrant miRNAs, validated the relevant expression of differentially expressed miRNAs, and predicted the possible miRNA targets and signaling pathways.

Results

The results indicated that 36 miRNAs were upregulated and 31 miRNAs were downregulated in EAU rats versus normal samples. Real-time quantitative PCR substantiated a high degree of confidence for the differentially expressed miRNAs, and miRNA analyses showed the differentially expressed miRNA targets were involved not only in the multicellular organismal process and developmental process, but also in T cell receptor signaling pathway, B cell receptor signaling pathway and so on.

Conclusions

Our findings show that the differentially expressed miRNAs in EAU rats were closely associated with immune signaling pathways and may be applied in early prevention, prognosis and possible therapy in uveitis, indicating that miRNAs play an important role in the development of uveitis.
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Metadaten
Titel
Characterization of microRNA expression profiling in peripheral blood lymphocytes in rats with experimental autoimmune uveitis
verfasst von
Dadong Guo
Jiao Li
Zhengfeng Liu
Kai Tang
Huixin Song
Hongsheng Bi
Publikationsdatum
01.09.2015
Verlag
Springer Basel
Erschienen in
Inflammation Research / Ausgabe 9/2015
Print ISSN: 1023-3830
Elektronische ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-015-0848-3

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