nach oben

Erschienen in:

01.03.2010 | Short Communication

Arabinoxylan-oligosaccharides (AXOS) reduce preneoplastic lesions in the colon of rats treated with 1,2-dimethylhydrazine (DMH)

verfasst von: Angelo Pietro Femia, Maddalena Salvadori, Willem F. Broekaert, Isabelle E. J. A. François, Jan A. Delcour, Christophe M. Courtin, Giovanna Caderni

Erschienen in: European Journal of Nutrition | Ausgabe 2/2010

Einloggen, um Zugang zu erhalten

Abstract

Background

Prebiotics are non-digestible compounds that beneficially affect the host by stimulating the growth and/or activity of one or a limited number of resident colonic bacteria in the gut. Reported beneficial effects of prebiotics include reduced gut infections, better absorption of minerals, and notably, antitumorigenic effects. Arabinoxylan (AX)-oligosaccharides (AXOS) have been suggested to exert prebiotic effects in the gut, but their effect on colon carcinogenesis has not been studied so far.

Aim of the study

To test the effect of AXOS in a rat colon carcinogenesis model.

Methods

We determined the occurrence of two types of preneoplastic lesions [aberrant crypt foci (ACF) and mucin depleted foci (MDF)] in the colon of rats treated with the colon carcinogen 1,2-dimethylhydrazine (DMH) and fed either a control diet or a diet containing AXOS (4.8% w/w) (15 rats in each group).

Results

Thirteen weeks after DMH treatment, MDF counts were significantly lower in the entire colon of AXOS fed rats (MDF/colon were 7.5 ± 0.6 and 5.5 ± 0.6, in Control and AXOS groups, respectively, means ± SE, P < 0.05). Although the number of ACF in the entire colon was not significantly different between Control and AXOS fed rats, AXOS fed rats had significantly fewer ACF in the distal part of the colon than Control group rats (ACF/distal colon were 135.5 ± 15 and 84.4 ± 11, in Control and AXOS groups, respectively, means ± SE, P < 0.05).

Conclusions

The present study shows that dietary intake of AXOS by rats reduces the occurrence of two types of preneoplastic lesions, thus suggesting a chemopreventive effect on colon carcinogenesis that should be confirmed in a long-term carcinogenesis experiment.

Andersson R, Aman P (2001) Cereal arabinoxylan: occurrence, structure and properties. In: McCleary BV, Prosky L (eds) Advanced dietary fibre technology. Blackwell Science Ltd, Oxford, pp 301–314

Anonymous (2000) Approved methods of the American Association of Cereal Chemists, 10th edn. AACC, St. Paul, MN, USA

AOAC (1995) Official methods of analysis, 16th edition. Method 990.03. Association of Official Analytical Chemists, WA, USA

Blumenkrantz N, Asboe-Hansen G (1973) New method for quantitative determination of uronic acids. Anal Biochem 54:484–489CrossRef

Boyle FG, Wrenn JM, Marsh BB, Anderson WI, Angelosanto FA, McCartney AL, Lien EL (2008) Safety evaluation of oligofructose: 13 week rat study and in vitro mutagenicity. Food Chem Toxicol 46:3132–3139CrossRef

Buddington KK, Donahoo JB, Buddington RK (2002) Dietary oligofructose and inulin protect mice from enteric and systemic pathogens and tumor inducers. J Nutr 132:472–477

Caderni G, Femia AP, Giannini A, Favuzza A, Luceri C, Salvadori M, Dolara P (2003) Identification of mucin-depleted foci in the unsectioned colon of azoxymethane-treated rats: correlation with carcinogenesis. Cancer Res 63:2388–2392

Campbell JM, Fahey GC Jr, Wolf BW (1997) Selected indigestible oligosaccharides affect large bowel mass, cecal and fecal short-chain fatty acids, pH and microflora in rats. J Nutr 127:130–136

Cleemput G, Roels SP, van Oort MG, Grobet PJ, Delcour JA (1993) Heterogeneity in the structure of water soluble arabinoxylans in European wheat flours of variable breadmaking quality. Cereal Chem 70:324–329

10.

Cloetens L, De Preter V, Swennen K, Broekaert WF, Courtin CM, Delcour JA, Rutgeerts P, Verbeke K (2008) Dose-response effect of arabinoxylo-oligosaccharides on gastrointestinal motility and on colonic bacterial metabolism in healthy volunteers. J Am Coll Nutr 27:512–518

11.

Corpet DE, Pierre F (2003) Point: from animal models to prevention of colon cancer. Systematic review of chemoprevention in min mice and choice of the model system. Cancer Epidemiol Biomarkers Prev 12:391–400

12.

Courtin CM, Swennen K, Broekaert WF, Swennen Q, Buyse J, Decuypere E, Michiels CW, De Ketelaere B, Delcour JA (2008) Effects of dietary inclusion of xylooligosaccharides, arabinoxylooligosaccharides and soluble arabinoxylan on the microbial composition of caecal contents of chickens. J Sci Food Agric 88:2517–2522CrossRef

13.

Courtin CM, Broekaert WF, Aerts G, Van Craeyveld V, Delcour JA (2009) Occurrence of arabinoxylo-oligosaccharides and arabinogalactan peptides in beer. J Am Soc Brew Chem 67:112–117

14.

Courtin CM, Swennen K, Broekaert WF, Lescroart O, Onagbesan O, Buyse J, Decuypere E, Van de Wiele T, Marzoratti M, Verstraete W, Huyghebaert G, Delcour JA (2008) Dietary inclusion of wheat bran arabinoxylooligosaccharides has beneficial nutritional effects on chickens. Cereal Chem 85:607–613CrossRef

15.

Eeckhaut V, Van Immerseel F, Dewulf J, Pasmans F, Haesebrouck F, Ducatelle R, Courtin CM, Delcour JA, Broekaert WF (2008) Arabinoxylooligosaccharides from wheat bran inhibit Salmonella colonization in broiler chickens. Poult Sci 87:2329–2334CrossRef

16.

European Community: European Community Regulations on the Care and Use of Laboratory Animals (1986) (Law 86/609/EC)

17.

Femia AP, Luceri C, Dolara P, Giannini A, Biggeri A, Salvadori M, Clune Y, Collins KJ, Paglierani M, Caderni G (2002) Antitumorigenic activity of the prebiotic inulin enriched with oligofructose in combination with the probiotics Lactobacillus rhamnosus and Bifidobacterium lactis on azoxymethane-induced colon carcinogenesis in rats. Carcinogenesis 23:1953–1960CrossRef

18.

Femia AP, Caderni G, Bottini C, Salvadori M, Dolara P, Tessitore L (2007) Mucin-depleted foci are modulated by dietary treatments and show deregulation of proliferative activity in carcinogen-treated rodents. Int J Cancer 120:2301–2305CrossRef

19.

Femia AP, Dolara P, Caderni G (2004) Mucin-depleted foci (MDF) in the colon of rats treated with azoxymethane (AOM) are useful biomarkers for colon carcinogenesis. Carcinogenesis 25:277–281CrossRef

20.

Femia AP, Giannini A, Fazi M, Tarquini E, Salvadori M, Roncucci L, Tonelli F, Dolara P, Caderni G (2008) Identification of mucin depleted foci in the human colon. Cancer Prev Res 1:562–567CrossRef

21.

Gibson GR, Roberfroid MB (1995) Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr 125:1401–1412

22.

Grootaert C, Van den Abbeele P, Marzorati M, Broekaert WF, Courtin CM, Delcour JA, Verstraete W, Van de Wiele T (2009) Comparison of prebiotic effects of arabinoxylan oligosaccharides and inulin in a simulator of the human intestinal microbial ecosystem. FEMS Microbiol Ecol 69:231–242

23.

Hartmann G, Piber M, Koehler P (2005) Isolation and chemical characterisation of water-extractable arabinoxylans from wheat and rye during breadmaking. Eur Food Res Technol 221:487–492CrossRef

24.

Hsu CK, Liao JW, Chung YC, Hsieh CP, Chan YC (2004) Xylooligosaccharides and fructooligosaccharides affect the intestinal microbiota and precancerous colonic lesions development in rats. J Nutr 134:1523–1528

25.

Izydorczyk MS, Biliaderis CG (1995) Cereal arabinoxylans: in structure and physicochemical properties. Carbohydr Polym 28:33–48CrossRef

26.

Katapodis P, Vardakou M, Kalogeris E, Kekos D, Macris BJ, Christakopoulos P (2003) Enzymic production of a feruloylated oligosaccharide with antioxidant activity from wheat flour arabinoxylan. Eur J Nutr 42:55–60CrossRef

27.

Kimura Y, Kono S, Toyomura K, Nagano J, Mizoue T, Moore MA, Mibu R, Tanaka M, Kakeji Y, Maehara Y, Okamura T, Ikejiri K, Futami K, Yasunami Y, Maekawa T, Takenaka K, Ichimiya H, Imaizumi N (2007) Meat, fish and fat intake in relation to subsite-specific risk of colorectal cancer: The Fukuoka Colorectal Cancer Study. Cancer Sci 98:590–597CrossRef

28.

Koushik A, Hunter DJ, Spiegelman D, Beeson WL, van den Brandt PA, Buring JE, Calle EE, Cho E, Fraser GE, Freudenheim JL, Fuchs CS, Giovannucci EL, Goldbohm RA, Harnack L, Jacobs DR Jr, Kato I, Krogh V, Larsson SC, Leitzmann MF, Marshall JR, McCullough ML, Miller AB, Pietinen P, Rohan TE, Schatzkin A, Sieri S, Virtanen MJ, Wolk A, Zeleniuch-Jacquotte A, Zhang SM, Smith-Warner SA (2007) Fruits, vegetables, and colon cancer risk in a pooled analysis of 14 cohort studies. J Natl Cancer Inst 99(19):1471–1483CrossRef

29.

Macfarlane GT, Steed H, Macfarlane S (2008) Bacterial metabolism and health-related effects of galacto-oligosaccharides and other prebiotics. J Appl Microbiol 104:305–344

30.

Ohta T, Semboku N, Kuchii A, Egashira Y, Sanada H (1997) Antioxidant activity of corn bran cell-wall fragments in the LDL oxidation system. J Agric Food Chem 45:1644–1648CrossRef

31.

Pierre F, Santarelli R, Taché S, Guéraud F, Corpet DE (2008) Beef meat promotion of dimethylhydrazine-induced colorectal carcinogenesis biomarkers is suppressed by dietary calcium. Br J Nutr 99:1000–1006CrossRef

32.

Plate AY, Gallaher DD (2006) Effects of indole-3-carbinol and phenethyl isothiocyanate on colon carcinogenesis induced by azoxymethane in rats. Carcinogenesis 27:287–292CrossRef

33.

Pretlow TP, Pretlow TG (2005) Mutant KRAS in aberrant crypt foci (ACF): initiation of colorectal cancer? Biochim Biophys Acta 1756:83–96

34.

Reddy BS, Hamid R, Rao CV (1997) Effect of dietary oligofructose and inulin on colonic preneoplastic aberrant crypt foci inhibition. Carcinogenesis 18:1371–1374CrossRef

35.

Rowland IR, Rumney CJ, Coutts JT, Lievense LC (1998) Effect of bifidobacterium longum and inulin on gut bacterial metabolism and carcinogen-induced aberrant crypt foci in rats. Carcinogenesis 19:281–285CrossRef

36.

Swennen K, Courtin CM, Lindemans GCJE, Delcour JA (2006) Large scale production and characterisation of wheat bran arabinoxylooligosaccharides. J Sci Food Agric 86:1722–1731CrossRef

37.

Van Craeyveld V, Swennen K, Dornez E, Van de Wiele T, Marzorati M, Verstraete W, Delaedt Y, Onagbesan O, Decuypere E, Buyse J, De Ketelaere B, Broekaert WF, Delcour JA, Courtin CM (2008) Structurally different wheat-derived arabinoxylooligosaccharides have different prebiotic and fermentation properties in rats. J Nutr 138:2348–2355CrossRef

38.

Van Loo J (2004) Prebiotics promote good health. The basis, the potential, and the emerging evidence. J Clin Gastroenterol 38:S70–S75CrossRef

39.

Verghese M, Rao DR, Chawan CB, Shackelford L (2002) Dietary inulin suppresses azoxymethane-induced preneoplastic aberrant crypt foci in mature Fisher 344 rats. J Nutr 132:2804–2808

40.

Wijnands MV, Schoterman HC, Bruijntjes JB, Hollanders VM, Woutersen RA (2001) Effect of dietary galacto-oligosaccharides on azoxymethane-induced aberrant crypt foci and colorectal cancer in Fischer 344 rats. Carcinogenesis 22:127–132CrossRef

41.

Yamada H, Itoh K, Morishita Y, Taniguchi H (1993) Structure and properties of oligosaccharides from wheat bran. Cereal Food World 38:490–492

42.

Younes H, Garleb K, Behr S, Rémésy C, Demigné C (1995) Fermentable fibers or oligosaccharides reduce urinary nitrogen excretion by increasing urea disposal in the rat cecum. J Nutr 125:1010–1016

Titel: Arabinoxylan-oligosaccharides (AXOS) reduce preneoplastic lesions in the colon of rats treated with 1,2-dimethylhydrazine (DMH)
verfasst von: Angelo Pietro Femia
Maddalena Salvadori
Willem F. Broekaert
Isabelle E. J. A. François
Jan A. Delcour
Christophe M. Courtin
Giovanna Caderni
Publikationsdatum: 01.03.2010
Verlag: Springer-Verlag
Erschienen in: European Journal of Nutrition / Ausgabe 2/2010
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-009-0050-x

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

Costims – das nächste heiße Ding in der Krebstherapie?

28.05.2024 Onkologische Immuntherapie Nachrichten

„Kalte“ Tumoren werden heiß – CD28-kostimulatorische Antikörper sollen dies ermöglichen. Am besten könnten diese in Kombination mit BiTEs und Checkpointhemmern wirken. Erste klinische Studien laufen bereits.

Positiver FIT: Die Ursache liegt nicht immer im Dickdarm

27.05.2024 Blut im Stuhl Nachrichten

Immunchemischer Stuhltest positiv, Koloskopie negativ – in solchen Fällen kann die Blutungsquelle auch weiter proximal sitzen. Ein Forschungsteam hat nachgesehen, wie häufig und in welchen Lokalisationen das der Fall ist.

GLP-1-Agonisten können Fortschreiten diabetischer Retinopathie begünstigen

24.05.2024 Diabetische Retinopathie Nachrichten

Möglicherweise hängt es von der Art der Diabetesmedikamente ab, wie hoch das Risiko der Betroffenen ist, dass sich sehkraftgefährdende Komplikationen verschlimmern.

Mehr Lebenszeit mit Abemaciclib bei fortgeschrittenem Brustkrebs?

24.05.2024 Mammakarzinom Nachrichten

In der MONARCHE-3-Studie lebten Frauen mit fortgeschrittenem Hormonrezeptor-positivem, HER2-negativem Brustkrebs länger, wenn sie zusätzlich zu einem nicht steroidalen Aromatasehemmer mit Abemaciclib behandelt wurden; allerdings verfehlte der numerische Zugewinn die statistische Signifikanz.

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Abstract

Background

Aim of the study

Methods

Results

Conclusions

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 2/2010

Very high plant stanol intake and serum plant stanols and non-cholesterol sterols

Astaxanthin suppresses scavenger receptor expression and matrix metalloproteinase activity in macrophages

Dietary sucrose intake is related to serum leptin concentration in overweight pregnant women

Lupin protein compared to casein lowers the LDL cholesterol:HDL cholesterol-ratio of hypercholesterolemic adults

Naringenin enhances insulin-stimulated tyrosine phosphorylation and improves the cellular actions of insulin in a dietary model of metabolic syndrome