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01.09.2005 | Original Article

Inter-laboratory validation of PCR-based detection of Mycobacterium tuberculosis in formalin-fixed, paraffin-embedded tissues

verfasst von: Christiane Schewe, Torsten Goldmann, Marianne Grosser, Albert Zink, Karsten Schlüns, Stefan Pahl, Timo Ulrichs, Stefan H. E. Kaufmann, Andreas Nerlich, Gustavo B. Baretton, Manfred Dietel, Ekkehard Vollmer, Iver Petersen

Erschienen in: Virchows Archiv | Ausgabe 3/2005

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Abstract

The present study is based on the initiative for quality assurance in pathology of the German Society of Pathology and the Professional Association of German Pathologists. Four panel laboratories with experience and expertise in polymerase chain reaction (PCR) detection of Mycobacterium tuberculosis were selected to establish the prerequisites for continuous external laboratory trials, in particular, by providing pre-tested specimens and evaluation criteria for participating institutes. In the first step, the four panel laboratories performed an internal trial to test their own reliability and reproducibility. Paraffin sections and DNA preparations from 34 tissues (25 clinical specimens and 9 controls) totalling to 66 samples were evaluated by each panel institute according to their own protocols. The methodologies differed and are described in detail. Despite these differences, a high degree of inter-laboratory reliability was achieved. In this report, we summarise our results including the correlation with the histology and provide recommendations for applying PCR-based methodology for the detection of mycobacterial DNA in surgical specimens. Supplementary data are available online at http://www.charite.de/ch/patho (rubric “Forschung”). Pre-tested specimens are now available for the external trial and can be ordered from the steering institute via Oligene (http://www.oligene.com/). All molecular pathology laboratories are invited to participate in this quality assurance initiative.

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Titel: Inter-laboratory validation of PCR-based detection of Mycobacterium tuberculosis in formalin-fixed, paraffin-embedded tissues
verfasst von: Christiane Schewe
Torsten Goldmann
Marianne Grosser
Albert Zink
Karsten Schlüns
Stefan Pahl
Timo Ulrichs
Stefan H. E. Kaufmann
Andreas Nerlich
Gustavo B. Baretton
Manfred Dietel
Ekkehard Vollmer
Iver Petersen
Publikationsdatum: 01.09.2005
Verlag: Springer-Verlag
Erschienen in: Virchows Archiv / Ausgabe 3/2005
Print ISSN: 0945-6317
Elektronische ISSN: 1432-2307
DOI: https://doi.org/10.1007/s00428-005-1233-3

Die Highlights vom Kongress des American College of Cardiology 2024

Springer Medizin

Inter-laboratory validation of PCR-based detection of Mycobacterium tuberculosis in formalin-fixed, paraffin-embedded tissues

Abstract

Neu im Fachgebiet Pathologie

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Die Highlights vom Kongress des American College of Cardiology 2024

Springer Medizin

Abstract

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