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22.09.2015 | Original Article

Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells

verfasst von: Manal Farea, Adam Husein, Ahmad Sukari Halim, Zurairah Berahim, Asma Abdullah Nurul, Khairani Idah Mokhtar, Kasmawati Mokhtar

Erschienen in: Clinical Oral Investigations | Ausgabe 6/2016

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Abstract

Objectives

The purpose of this study was to evaluate the synergistic effect of epithelial rests of Malassez cells (ERM) and transforming growth factor-β₁ (TGF-β₁) on proliferation, cementogenic and osteogenic differentiation of stem cells derived from human exfoliated deciduous teeth (SHED).

Materials and methods

SHED were co-cultured with ERM with/without TGF-β₁. Then, SHED proliferation, morphological appearance, alkaline phosphatase (ALP) activity, mineralization behaviour and gene/protein expression of cemento/osteoblastic phenotype were evaluated.

Results

TGF-β₁ enhanced SHED proliferation when either cultured alone or co-cultured with ERM. ERM induced the cementoblastic differentiation of SHED which was significantly accelerated when treated with TGF-β₁. This activity was demonstrated by high ALP activity, strong mineral deposition and upregulation of cementum/bone-related gene and protein expressions (i.e. ALP, collagen type I, bone sialoprotein, osteocalcin and cementum attachment protein).

Conclusions

ERM were able to induce SHED differentiation along the cemento/osteoblastic lineage that was triggered in the presence of TGF-β₁.

Clinical relevance

The cemento/osteoblastic differentiation capability of SHED possesses a therapeutic potential in endodontic and periodontal tissue engineering.

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Titel: Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells
verfasst von: Manal Farea
Adam Husein
Ahmad Sukari Halim
Zurairah Berahim
Asma Abdullah Nurul
Khairani Idah Mokhtar
Kasmawati Mokhtar
Publikationsdatum: 22.09.2015
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 6/2016
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-015-1601-6

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Cementoblastic lineage formation in the cross-talk between stem cells of human exfoliated deciduous teeth and epithelial rests of Malassez cells