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Germinated Brown Rice Extract Inhibits Adipogenesis Through the Down-regulation of Adipogenic Genes in 3T3-L1 Adipocytes

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Abstract

The aim of this study was to examine the anti-adipogenic effect of germinated brown rice methanol extract (GBR) in 3T3-L1 adipocytes. The GBR inhibited adipocyte differentiation was measured by Oil Red O staining and glycerol-3-phosphate dehydrogenase (GPDH) activity in a dose-dependent manner without initiating any cytotoxicity. The mRNA levels of adipogenic transcription factors such as CCAAT/enhancer binding protein (C/EBPα), proliferator-activated receptorγ (PPARγ), and sterol regulatory element-binding protein-1c (SREBP-1c), and adipogenic genes, such as fatty acid synthase (FAS), adipocyte fatty acid-binding protein (aP2), and lipoprotein lipase (LPL), were significantly down-regulated by treatment with GBR when compared to that of untreated control cells. Moreover, tumor necrosis factor-α (TNF-α) and interlukin-6 (IL-6) mRNA expressions were attenuated by GBR in mature adipocytes. These data suggest that GBR exhibits an anti-adipogenic effect through the suppression of adipogenesis in 3T3-L1 adipocytes.

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Abbreviations

aP2:

Adipocyte fatty acid-binding protein

BR:

Brown rice

C/EBPα:

CCAAT/enhancer binding protein

FAS:

Fatty acid synthase

GBR:

Germinated brown rice

GPDH:

Glycerol-3-phosphate dehydrogenase

IL-6:

Interlukin-6

PPARγ:

Proliferator-activated receptorγ

SREBP-1c:

Sterol regulatory element-binding protein-1c

ATGL:

Adipose triglyceride lipase

HSL:

Hormone sensitive lipase

LPL:

Lipoprotein lipase

TNF-α:

Tumor necrosis factor-α

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Acknowledgments

This work was supported by the Grant of the Korean Research Foundation in the Korean Ministry of Education, Science and Technology (code 2008–0060892).

Conflict of Interest

The authors have no conflict of interest.

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Authors and Affiliations

  1. Department of Medical Nutrition, Kyung Hee University, Yongin, 446-701, Korea

    Jin-Nyoung Ho

  2. Department of Food and Biotechnology, Korea University, Sejong, 339-700, Korea

    Mi-Eun Son, Won-Chul Lim & Hong-Yon Cho

  3. Department of Food Technology, Korea University, Seoul, 136-713, Korea

    Seung-Taik Lim

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Correspondence to Hong-Yon Cho.

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Ho, JN., Son, ME., Lim, WC. et al. Germinated Brown Rice Extract Inhibits Adipogenesis Through the Down-regulation of Adipogenic Genes in 3T3-L1 Adipocytes. Plant Foods Hum Nutr 68, 274–278 (2013). https://doi.org/10.1007/s11130-013-0366-9

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