Introduction
Several bariatric surgical techniques originally designed to promote weight loss offer a variable but impressive rate of cure for type 2 diabetes mellitus (T2DM). In 80% of patients who undergo Roux-en-Y gastric bypass surgery (RYGB), T2DM resolves or improves significantly.
1,
2 RYGB is the most common bariatric surgical technique used in the USA and seems to provide better weight loss and higher rates of resolution of T2DM than purely restrictive techniques such as laparoscopic gastric banding.
3‐
5
The proposed mechanisms to account for why RYGB offers this remarkable rate of resolution of T2DM have been under extensive scrutiny in both animals and humans.
1,
6‐
12 At the center of the debate is the relative contribution of greater and sustained weight loss, or to an altered pattern of gut and pancreatic hormone secretion, frequently called the “incretin effect”, or other factors.
13,
14 RYGB creates an anatomical rearrangement that delivers a partially digested food bolus directly into the second portion of the small bowel while avoiding contact with a large portion of the stomach and the duodenum.
15‐
17 This, in turn, results in altered glucose kinetics
18 and altered secretion of many gut and pancreatic hormones known to affect glucose metabolism
6,
19 and has additional effects on gastric emptying
20 and on neurohormonal gut–brain signaling that regulates energy homeostasis and hunger–satiety mechanisms.
10,
20,
21 However, to date, there have been few detailed and controlled metabolic studies of the interplay and independent effects of RYGB on the many factors that affect insulin resistance and glucose metabolism, such as beta cell function, the associated changes on gut and pancreatic hormone levels, the magnitude and rate of weight loss, energy balance, changes in body composition, and other factors. Information from such studies might help clinicians and patients in choosing among available surgical treatments for morbid obesity and guide the search for novel surgical procedures to treat obesity-associated T2DM in patients with lower body mass indices (BMI). Therefore, the goal of this study was to delineate short-term changes in total body glucose disposal, gut and pancreatic hormone secretion, and body composition, while controlling for energy balance and delineate the same changes after more substantial weight loss had occurred 6 months after RYGB.
Patients and Methods
Morbidly obese patients, selected to undergo gastric bypass surgery (RYGB), were recruited at the University of California, San Francisco’s (UCSF) Bariatric Surgery Program. They met the National Institute of Health and UCSF Bariatric Surgery Program criteria for bariatric surgery: age 21 to 65 years old, BMI either >40 or >35 kg/m2 with high-risk comorbidities, documented desire to undergo bariatric surgery, well informed and motivated, acceptable operative risks, evaluated and cleared for the procedure by a certified dietitian and a psychiatrist or psychologist, documented repeated failure of nonsurgical supervised weight loss programs, documented BMI >35 kg/m2 for more than 5 years, and ability and willingness to provide informed consent. Exclusion criteria included previous weight loss surgery; a previous esophageal, gastric, pancreatic, adrenal, small bowel, large bowel, thyroid, or central nervous system operation; diagnosis of thyroid, liver, pancreatic, adrenal, hypothalamic, pituitary, ovarian, and chronic renal disease; diagnosis of T2DM; use of insulin or any oral medications for T2DM; or unwillingness or inability to give informed consent. This project was approved by the UCSF Committee of Human Research and the UCSF Clinical and Translational Science Institute Clinical Research Center (CRC) Advisory Committee.
Twenty-two patients were allocated to two groups: One underwent immediate laparoscopic RYGB surgery followed by standardized calorie restriction (RYGB, N = 12), and the other underwent caloric restriction only (diet, N = 10). Allocation to either group was determined by randomization in the initial 17 patients studied and then by CRC and surgery date availability in the last five patients.
All participants underwent the same baseline metabolic evaluation (visit 1, V1). Subjects were admitted to the CRC at 7:00 p.m. on study day 0 for an initial complete medical history and physical examination. On that day, they began an “ad libitum” diet up to a maximum intake of 25 kcal/kg/24 h. Each morning upon arising, they were weighed on a calibrated scale after voiding.
Statistical Analysis
Data are summarized as mean and standard deviation unless otherwise stated. The unadjusted association of proportions and the distribution of continuous variables between groups and the association of each variable with outcomes were determined by two-sided t test and chi-square tests. Area under the curve (AUC) was calculated using the trapezoidal rule. Linear associations were measured using the Pearson’s correlation coefficient. Statistical significance was considered to be p < 0.05. SPSS, version 13.0.1 (SPSS Inc, Chicago, IL, USA), was used for all statistical analyses.
Discussion
Operations such as RYGB that bypass the duodenum and/or stomach, thus allowing for early delivery of the food bolus to the small intestine and preventing food bolus contact with the duodenum, offer a unique opportunity for identifying weight loss independent mechanisms for resolution of diabetes.
9 Consequently, a variety of novel surgical and endoscopic gastrointestinal procedures are under investigation for use as therapeutic options in treating T2DM.
27‐
31 Critical unanswered questions remain as to whether or to what degree the altered patterns of gut and pancreatic hormone secretion known to occur with bypass operations bolster beta cell function and ultimately improve peripheral glucose disposal and promote resolution of T2DM independent of weight loss.
9 The fact remains, however, that bariatric operations that result in greater weight loss are still associated with greater rates of T2DM improvement or cure.
1 In a recent systematic review and meta-analysis, Buchwald and colleagues
1 showed that excess weight loss and diabetes resolution in the first 12 months after surgery were the highest for patients undergoing biliopancreatic diversion/duodenal switch (BPD/DS; 64% excess weight loss, 95% diabetes resolution) followed by RYGB (60% excess weight loss, 80% diabetes resolution) and the least for banding procedures (46% excess weight loss, 57% diabetes resolution). However, BPD/DS and RYGB may impact and alter mechanisms other than weight loss that play an independent role in improving insulin resistance and allow for this high rate of resolution of T2DM. The intricate interplay among insulin sensitivity/resistance, glucose metabolism, and insulin secretion is affected by many factors: beta cell function; quality, distribution, and total fat mass; energy balance and the magnitude of calorie restriction; hepatic glucose and insulin metabolism and kinetics; quality and quantity of nutrient intake and absorption; associated diseases and stressors such as sleep apnea, liver fat, and adipocytokines; and gut and pancreatic hormone secretion and metabolism, among others.
9,
32
In this study, we sought to delineate short-term changes in peripheral glucose disposal, fasting measures of glucose and insulin, gut and pancreatic hormone secretion in response to a meal challenge, and body composition, while controlling for energy balance, and to evaluate these same changes after more substantial weight loss had occurred 6 months after RYGB. We confirmed previous studies in showing that, after RYGB, the pattern of gut and pancreatic hormone secretion following a meal is altered compared to controls
10,
11,
30,
33 and that RYGB patients have early improvements in fasting glucose, fasting insulin, and, thus, in calculated HOMA-IR. Notably, the magnitude of improvement in fasting glucose homeostasis was similar in the group that underwent diet alone, indicating that weight loss, rather than the surgical procedure, results in these early changes. We also found that soon after RYGB and before massive weight loss occurs, peripheral glucose disposal (measured by euglycemic–hyperinsulinemic clamp) was not improved. Taken together, these results suggest that in the nondiabetic morbidly obese with severe insulin resistance, the short-term effects of gastric bypass surgery can improve glucose homeostasis under fasting conditions before substantial weight loss occurs but are not sufficient to improve peripheral glucose disposal during hyperinsulinemia. Other investigators, using a variety of techniques to study glucose metabolism, have documented improvements in insulin resistance from 1 week to 1 month after BPD/DS
34 and also RYGB with weight loss varying from 9 to 15 kg.
34‐
37 In contrast to our study, these studies were done in diabetic subjects and most were performed without a diet-only control group. Nevertheless, these differences support the notion that diabetic patients may respond differently to these procedures than nondiabetics.
The altered pattern of gut hormone secretion may result in amelioration of glucose disposal independently of weight loss by many mechanisms. For example, GLP-1 reduces elevated fasting and postprandial blood glucose levels in diabetic humans; leads to glucose-dependent insulin secretion, induction of beta-cell proliferation and expansion of the beta-cell mass, and enhanced resistance to beta-cell apoptosis; inhibits gastric emptying and acid secretion; and reduces food ingestion and glucagon secretion, among other functions.
20,
38 We and others demonstrated that RYGB is associated with an increased release of postprandial GLP-1.
13,
20,
33,
39 While the more rapid delivery of glucose and other nutrients to the proximal intestine may partially explain the rapid and robust insulin and GLP-1 responses following RYGB, the magnitude of the exaggeration of the GLP-1 response suggests that other unique features with RYGB also contribute to this increase. In another example, GIP, which is released from the duodenum and proximal small bowel K-cells in response to glucose and fat ingestion, augments glucose-stimulated insulin secretion in healthy humans but almost completely loses its insulinotropic effect in patients with T2DM.
20,
33 Others have shown a blunted recovery of GIP levels in obese diabetic patients, but not in obese nondiabetics, after RYGB.
17,
33,
40 Patients in our current study, although not classified as diabetics, had markedly impaired glucose disposal, and the recovery of GIP secretion in diet-only subjects after minimal weight loss and maintenance of low levels of GIP in the RYGB group were similar to those observed in diabetics in other studies.
17,
33,
40
A unique strength of our study was the use of the hyperinsulinemic–euglycemic clamp and repeated testing under controlled conditions. The clamp technique is widely accepted as the reference standard for directly determining insulin sensitivity and peripheral glucose disposal in humans
41 and offers, in the research setting, significant advantages over the commonly used technique for assessing insulin sensitivity, such as HOMA-IR. The hyperinsulinemic–euglycemic clamp leads to a steady-state condition in which the glucose infusion rate during the clamp must be equal to the glucose disposal rate. Thus, the clamp estimates insulin sensitivity/resistance in humans and directly measures peripheral glucose disposal at a given level of insulinemia under steady-state conditions.
22,
41 In addition, the glucose clamp has excellent test characteristics. Peripheral glucose uptake typically has a coefficient of variation of 0.10 and a discriminant ratio of 6 (a measurement of both reproducibility and the ability to distinguish individual results).
42 On the other hand, HOMA-IR, which has been used in many other studies of the metabolic effects of bariatric surgery, is a simple surrogate index for insulin resistance that is derived from blood insulin and glucose concentrations under fasting conditions and reflects mostly hepatic insulin sensitivity. The main limitations of the glucose clamp approach are that it is time-consuming, labor-intensive, expensive, and requires an experienced operator to manage the technical difficulties, but these were overcome by the use of CRC resources and the experienced group of endocrinologists. Moreover, because glucose and insulin are administered parenterally during the clamp, thus bypassing the gut and splanchnic metabolism, this technique measured peripheral glucose uptake independent of any potential influence of altered nutrient delivery to the small intestine or changes in incretin secretion following RYGB.
Evidence from our study and others leaves little doubt that calorie restriction alone can improve fasting glucose and insulin levels and is an important factor leading to the rapid changes observed after RYGB. However, as detailed above, RYGB provides mechanisms that are independent of weight loss and impact and/or bolster the ability of morbidly obese patients to experience a more rapid amelioration of glucose metabolism. However, fasting glucose and insulin measurements, which are usually obtained as a surrogate for insulin sensitivity in most studies performed to date and were the measures that improved similarly at 14 days after RYGB or calorie restriction only, are mostly a reflection of hepatic insulin sensitivity. The M value obtained during the clamp study provides the best estimate of peripheral glucose homeostasis, and as we demonstrate, M values improved only after significant weight loss and correlated with the magnitude of weight loss.
Limitations of our study include small differences between RYGB and diet groups in gender distribution, weight loss, percentage of weight loss as fat, average daily energy intake, and possible differences in diet absorption that, although not statistically significant, when combined may have impacted the results. Nevertheless, these differences in changes would have led to improvement in glucose metabolism in the RYGB group. On the other hand, the surgical insult and trauma of a laparoscopic operation for RYGB may have had a negative impact, in the 14 day analyses, on glucose homeostasis and disposal. Lastly, we may have not been able to identify a differential and earlier improvement in peripheral glucose disposal in the RYGB group because we studied only nondiabetic participants and studied them only at three time points. Although our participants were profoundly insulin resistant, they may have had effective counter-regulatory mechanisms that successfully forestalled progression to diabetes, and thus, we may have observed a different result had we studied patients with diabetes.
With these limitations in view, we conclude that in nondiabetic morbidly obese subjects under similar caloric restriction and weight loss, peripheral glucose disposal is not improved early after RYGB or calorie restriction. Improvement in peripheral glucose disposal following RYGB was observed only after substantial weight loss had occurred and correlated with the magnitude of weight lost. These findings suggest that weight loss is a critical component for complete restoration of glucose homeostasis in the morbidly obese with insulin resistance.
Acknowledgments
This publication was supported by Grant Number KL2 RR024130 from the National Center for Research Resources (NCRR), a component of the NIH and NIH Roadmap for Medical Research (GMC), and by NIH/NCRR UCSF-CTSI Grant Number UL1 RR024131. We are also grateful for the assistance of the SFGH-CRC nursing, dietary, and laboratory staff, especially Laurie Herraiz, RD, Veronica Monti, RD, and Viva Tai, MPH, RD; James Graham, PhD, of the University of California Davis, who coordinated the hormone analyses; and Eric Vittinghoff, PhD, for assistance with the statistical analyses.
Discussant
Dr. Bruce M. Wolfe (Portland, OR): Dr. Campos and colleagues demonstrated that 2 weeks postgastric bypass, GLP-1 and insulin secretion were enhanced, but peripheral insulin resistance as reflected by glucose uptake did not decrease until substantial weight loss occurred at 6 months postoperatively. I have the following questions: First, you indicated that all subjects demonstrated insulin resistance prior to operations. Would you comment on the importance of this finding?
Second, HOMA, a measure of insulin resistance calculated from fasting glucose and insulin decreased at 2 weeks, but the clamp derived M score of glucose uptake and peripheral insulin resistance did not. Is HOMA a useful parameter in these studies or will further studies require the extensive and burdensome clamp studies?
Finally, are you confident that the insulin infusion was effective in shutting down hepatic gluconeogenesis allowing you to draw conclusions regarding peripheral glucose uptake?
Closing Discussant
Dr. Guilherme M. Campos (San Francisco, CA): Thank you, Dr. Wolfe. Your questions highlight the need to clearly understand the definition of diabetes and prediabetes and that diabetes is a spectrum of a disease. What is interesting in our patient population of morbidly obese individuals is that none had a diagnosis of diabetes. All their fasting glucose measures were below 105 and with normal hemoglobin A1c, thus fitting the current criteria for nondiabetics. However, when we studied and challenged them using the hyperinsulinemic–euglycemic clamp, they uniformly had peripheral insulin resistance and poor peripheral glucose disposal. How to incorporate and apply these tests in other studies? The clamp technique is widely accepted as the reference standard for directly determining insulin sensitivity and peripheral glucose disposal or uptake in humans and offers, in the research setting, significant advantages over the commonly used technique for assessing insulin sensitivity, such as fasting measurements like HOMA-IR. However, it cannot be used in large epidemiological studies as it is indeed labor-intensive and expensive. Thus, surrogate indices of insulin resistance, such as HOMA-IR, are an acceptable alternative, but as shown in our study, it will not identify all patients with impaired glucose metabolism.
Lastly, was the amount of insulin chosen during the clamp effective in suppressing hepatic gluconeogenesis?—the answer is we do not know. We would have to have radiolabeled glucose tracer studies to study hepatic glucose production to see if the effect was indeed enough to suppress hepatic gluconeogenesis. But that does not affect our results and conclusions as we use the same amount of intravenous insulin in all three evaluations using standard dosing per square meter. So, even if hepatic gluconeogenesis was not suppressed, the observed values and changes are still valid and reliable.
Discussant
Dr. Nils Lambrecht (Los Angeles, CA): Do you have any data on gastric sleeve surgery because the physical removal of a large portion of the gastric oxyntic mucosa including most ghrelin containing endocrine cells may play a big role in changes in food intake and dietary behavior?
Closing Discussant
Dr. Guilherme M. Campos (San Francisco, CA): No, we do not have data on gastric sleeve.