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Basic Research in Cardiology

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01.09.2012 | Original Contribution

Adipocytes modulate the electrophysiology of atrial myocytes: implications in obesity-induced atrial fibrillation

verfasst von: Yung-Kuo Lin, Yao-Chang Chen, Jenn-Han Chen, Shih-Ann Chen, Yi-Jen Chen

Erschienen in: Basic Research in Cardiology | Ausgabe 5/2012

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Abstract

Obesity is an important risk factor for atrial fibrillation (AF). Increased epicardial adipose tissue in obesity can enhance inflammation and plays an important role in the pathophysiology of AF. However, it is not clear whether epicardial adipocytes directly modulate the electrophysiological characteristics of atrial myocytes. Whole-cell patch clamp was used to record the action potentials (APs) and ionic currents in isolated rabbit left atrium (LA) myocytes incubated with and without (control) isolated adipocytes from epicardial, retrosternal, or abdominal adipose tissues, or adipocytes-conditioned supernatant for 2–4 h. Compared to control LA myocytes (n = 22), LA myocytes incubated with epicardial (n = 17), retrosternal (n = 18), or abdominal adipocytes (n = 22) had longer (80 ± 3, 109 ± 6, 109 ± 6, and 110 ± 7 ms, p < 0.001) 90 % AP durations (APD₉₀). LA myocytes incubated with epicardial adipocytes had a more-positive resting membrane potential (RMP) than control LA myocytes (−57 ± 1 mV vs. −63.4 ± 1.4 mV, p < 0.05). However, LA myocytes (n = 32) incubated with supernatant had longer APD₉₀ (93 ± 3 ms, p < 0.05), but similar RMP values (−62 ± 2 mV, p > 0.05) in comparison to control myocytes. Epicardial adipocyte-incubated LA myocytes had larger late sodium currents, L-type calcium currents, and transient outward potassium currents, but smaller delayed rectifier potassium and inward rectifier potassium currents than control LA myocytes. Moreover, isoproterenol (10 nM) induced a higher incidence (67 vs. 22 %, p < 0.05) of triggered beats in adipocytes-incubated LA myocytes (n = 12) than in control LA myocytes (n = 9). In conclusion, adipocytes can directly modulate the electrophysiological properties and ion currents, causing higher arrhythmogenesis in LA myocytes.

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Titel: Adipocytes modulate the electrophysiology of atrial myocytes: implications in obesity-induced atrial fibrillation
verfasst von: Yung-Kuo Lin
Yao-Chang Chen
Jenn-Han Chen
Shih-Ann Chen
Yi-Jen Chen
Publikationsdatum: 01.09.2012
Verlag: Springer-Verlag
Erschienen in: Basic Research in Cardiology / Ausgabe 5/2012
Print ISSN: 0300-8428
Elektronische ISSN: 1435-1803
DOI: https://doi.org/10.1007/s00395-012-0293-1

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Adipocytes modulate the electrophysiology of atrial myocytes: implications in obesity-induced atrial fibrillation

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