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01.03.2005 | Original Article

Establishment and characterization of the permanent human cell line C3842 derived from a secondary chondrosarcoma in Ollier’s disease

verfasst von: Thomas Kalinski, Sabine Krueger, Antje-Friederike Pelz, Peter Wieacker, Roland Hartig, Martin Röpke, Regine Schneider-Stock, Frank Dombrowski, Albert Roessner

Erschienen in: Virchows Archiv | Ausgabe 3/2005

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Abstract

The permanent human cell line C3842 was established from a secondary chondrosarcoma in a typical case of Ollier’s disease. In the present study, we analyzed the morphological, cytogenetic and molecular biological characteristics of the cultured cells in comparison with the original tumor and investigated the invasion properties of the tumor model using functional imaging of proteolysis, matrigel assay and chick chorioallantoic membrane assay. C3842 cells exhibit the typical features of malignant cartilage tumor cells in vitro, including the expression of collagen types II, IX, XI and aggrecan. The proteolytic ability of C3842 cells is attributed to the expression of several proteases, such as cathepsin B, urokinase plasminogen activator and matrix-metalloproteinase-2, which enable the cells to degrade collagen type I and to permeate matrigel matrix. In accordance with the biological features in vivo, C3842 cells are not able to invade through the epithelium of the chick chorioallantoic membrane. In conclusion, the cell line C3842 provides the first model of a secondary chondrosarcoma in Ollier’s disease in vitro, which is characterized by distinct features of such malignant cartilage tumors.

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Titel: Establishment and characterization of the permanent human cell line C3842 derived from a secondary chondrosarcoma in Ollier’s disease
verfasst von: Thomas Kalinski
Sabine Krueger
Antje-Friederike Pelz
Peter Wieacker
Roland Hartig
Martin Röpke
Regine Schneider-Stock
Frank Dombrowski
Albert Roessner
Publikationsdatum: 01.03.2005
Verlag: Springer-Verlag
Erschienen in: Virchows Archiv / Ausgabe 3/2005
Print ISSN: 0945-6317
Elektronische ISSN: 1432-2307
DOI: https://doi.org/10.1007/s00428-004-1194-y

Die Highlights vom Kongress des American College of Cardiology 2024

Springer Medizin

Establishment and characterization of the permanent human cell line C3842 derived from a secondary chondrosarcoma in Ollier’s disease

Abstract

Neu im Fachgebiet Pathologie

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Die Highlights vom Kongress des American College of Cardiology 2024

Springer Medizin

Abstract

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