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Role of MAPK in mechanical force-induced up-regulation of type I collagen and osteopontin in human gingival fibroblasts

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Abstract

In addition to periodontal ligament, the gingival plays an important role in alveolar bone remodeling induced by physiological and mechanical stimuli. However, there are few reports showing the cellular responses of human gingival fibroblasts (HGF) to a mechanical force. This study examined the effects of centrifugal force on the proliferation of the bone tissue components, such as type I collagen (COL I), osteopontin (OPN), and osteonectin (ONN) in the HGF. The roles of extracellular signal-regulated kinase (ERK), c-Jun-N-terminal kinase (JNK), and p-38 kinase were also investigated. Centrifugal force induced cell cycle arrest in the G1 phase without any cytotoxic effects and increased the levels of COL I and OPN expression in the cells but had no effect on ONN. The force-induced up-regulation of COL I was found to be mediated by both the ERK-c-Fos-COL I and JNK-c-Jun-COL I pathways, while that of OPN was mediated only by the ERK-mediated pathway. Our present findings suggest that centrifugal force up-regulates COL I and OPN expression in HGF, where both ERK and JNK play indispensable roles.

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Acknowledgment

This work was supported by Korea Research Foundation Grant endowed to RCBM in Chonbuk National University (KRF-2006-005-J03102).

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Authors and Affiliations

  1. School of Dentistry and Institute of Oral Biosciences, Chonbuk National University, Jeonju, 561-756, South Korea

    Young-Mi Jeon, Sung-Ho Kook, Soon-Sun Park, Jong-Ghee Kim & Jeong-Chae Lee

  2. Division of Biological Sciences, Chonbuk National University, Jeonju, 561-756, South Korea

    Sung-Ho Kook

  3. Department of Bioactive Materials and Research Center of Bioactive Materials, Chonbuk National University, Jeonju, 561-756, South Korea

    Young-Ok Son, Eun Mi Kim & Jeong-Chae Lee

  4. Graduate Center for Toxicology, University of Kentucky, Lexington, KY, 40536-0001, USA

    Young-Ok Son

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  1. Young-Mi Jeon
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  2. Sung-Ho Kook
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Correspondence to Jeong-Chae Lee.

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Young-Mi Jeon and Sung-Ho Kook contributed equally to this work.

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Jeon, YM., Kook, SH., Son, YO. et al. Role of MAPK in mechanical force-induced up-regulation of type I collagen and osteopontin in human gingival fibroblasts. Mol Cell Biochem 320, 45–52 (2009). https://doi.org/10.1007/s11010-008-9897-z

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  • DOI: https://doi.org/10.1007/s11010-008-9897-z

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