Abstract
Vascular endothelial cell activation by cytokines and other pro-inflammatory mediators is an initial event in atherosclerosis and in other vascular diseases. Simvastatin, a HMG-CoA reductase inhibitor, suppressed both tumor necrosis factor (TNF)-α-and angiotensin (Ang) II-induced monocyte adhesion to endothelial cells (an initial step in vascular inflammation) and reactive oxygen species (ROS) production. Diphenyleneiodonium and apocynin, both NADPH oxidase inhibitors, also suppressed TNF-α-induced ROS and monocyte-endothelial cell adhesion, demonstrating that TNF-α-induced monocyte adhesion is mediated through ROS produced by NADPH oxidase activation. Furthermore, exogenously applied mevalonate or geranylgeranylpyrophosphate in combination with simvastatin completely prevented the inhibitory effects of simvastatin on ROS generation and monocyte-endothelial cell adhesion by TNF-α and Ang II. These results suggest that monocyte adhesion to endothelial cells induced by TNF-α or Ang II is mediated via the geranylgeranyl isoprenoid-dependent generation of ROS, and that this is inhibited by simvastatin.
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Alexander, R. W., Hypertension and pathogenesis of atherosclerosis. Oxidative stress and the mediation of arterial inflammatory response: a new perspective. Hypertension, 25, 155–161 (1995).
Akgur, F. M., Brown, M. F., Zibari, G. B., McDonald, J. C., Epstein, C. J., Ross, C. R., and Granger, D. N., Role of superoxide in hemorrhagic shock-induced P-selectin expression. Am. J. Physiol. Heart Circ. Physiol., 279, H791–H797 (2000).
Cai, H. and Harrison, D. G.., Endothelial dysfunction in cardiovascular diseases: the role of oxidant stress. Circ. Res., 87,840–844 (2000).
Capers, Q. 4th., Alexander, R. W., Lou, P., De Leon, H., Wilcox, J. N., Ishizaka, N., Howard, A. B., and Taylor, W. R., Monocyte chemoattractant protein-1 expression in aortic tissues of hypertensive rats. Hypertension, 30, 1397–1402 (1997).
Carvalho, D., Savage, C. O., Black, C. M., and Pearson, J.D., IgG antiendothelial cell autoantibodies from scleroderma patients induce leukocyte adhesion to human ascular endothelial cells in vitro. Induction of adhesion molecule expression and involvement of endothelium-derived cytokines. J. Clin. Invest., 97, 111–119 (1996).
Chen, X. L., Tummala, P. E., Olbrych, M. T., Alexander, R. W., and Medford, R. M., Angiotensin II induces monocyte chemoattractant protein-1 gene expression in rat vascular smooth muscle cells. Circ. Res., 83, 952–959 (1998).
Chang, L. T., Sun, C. K., Chiang, C. H., Wu, C. J., Chua, S., and Yip, H. K., Impact of simvastatin and losartan on antiinflammatory effect: in vitro study. J. Cardiovasc. Pharmacol., 49,20–26 (2007).
Collins, T., Endothelial nuclear factor-êB and the initiation of the atherosclerotic lesion. Lab. Invest., 68, 499–508 (1993).
Cooper, D., Stokes, K. Y., Tailor, A., and Granger, D. N., Oxidative stress promotes blood cell-endothelial cell interactions in the microcirculation. Cardiovasc. Toxicol., 2, 165–180 (2002).
Cybulsky, M. I. and Gimbrone, M. A. Jr., Endothelial expression of a mononuclear leukocyte adhesion molecule during atherogenesis. Science, 251, 788–791 (1991).
DiDonato, J. A., Hayakawa, M., Rothwarf, D. M., Zandi, E., and Karin, M., A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappa B. Nature, 388,548–554 (1997).
Dzau, V. J., Theodore Cooper lecture: tissue angiotensin and pathobiology of vascular disease: a unifying hypothesis. Hypertension, 37, 1047–1052 (2001).
El Oualid, F., Cohen, L. H., van der Marel, G. A., and Overhand, M., Inhibitors of protein: geranylgeranyl transferases. Curr. Med. Chem., 13, 2385–2427 (2006).
Fiers, W., Beyaert, R., Declercq, W., and Vandenabeele, P., More than one way to die: apoptosis, necrosis and reactive oxygen damage. Oncogene, 18, 7719–7730 (1999).
Goossens, V., Stangé, G., Moens, K., Pipeleers, D., and Grooten, J., Regulation of tumor necrosis factor-induced, mitochondriaand reactive oxygen species-dependent cell death by the electron flux through the electron transport chain complex I. Antioxid. Redox Signal., 1, 285–295 (1999).
Grisham, M. B., Granger, D. N., and Lefer, D. J., Modulation of leukocyte-endothelial interactions by reactive metabolites of oxygen and nitrogen: relevance to ischemic heart disease. Free Radic. Biol. Med., 25, 404–433 (1998).
Granger, D. N., Benoit, J. N., Suzuki, M., and Grisham, M. B., Leukocyte adherence to venular endothelium during ischemiareperfusion. Am. J. Physiol., 257, G683–G688 (1989).
Harris, P. and Ralph, P., Human leukemic models of myelomonocytic development: a review of the HL-60 and U937 cell lines. J. Leukoc. Biol., 37, 407–422 (1985).
Harrison, D. G., Cai, H., Landmesser, U., and Griendling, K. K., Interactions of angiotensin II with NAD(P)H oxidase, oxidant stress and cardiovascular disease. J. Renin Angiotensin Aldosterone Syst., 4, 51–61 (2003).
Kaikita, K., Hayasaki, T., Okuma, T., Kuziel, W. A., Ogawa, H., and Takeya, M., Targeted deletion of CC chemokine receptor 2 attenuates left ventricular remodeling after experimental myocardial infarction. Am. J. Pathol., 165, 439–447 (2004).
Kaneyuki, U., Ueda, S., Yamagishi, S., Kato, S., Fujimura, T., Shibata, R., Hayashida, A., Yoshimura, J., Kojiro, M., Oshima, K., and Okuda, S., Pitavastatin inhibits lysophosphatidic acid-induced proliferation and monocyte chemoattractant protein-1 expression in aortic smooth muscle cells by suppressing Rac-1-mediated reactive oxygen species generation. Vascul. Pharmacol., 46, 286–292 (2007).
Kiliszek, M., Maczewski, M., Styczynski, G., Duda, M., Opolski, G., and Beresewicz, A., Low-density lipoprotein reduction by simvastatin is accompanied by angiotensin II type 1 receptor downregulation, reduced oxidative stress, and improved endothelial function in patients with stable coronary artery disease. Coron Artery Dis., 18, 201–209 (2007).
Kim, Y. S., Morgan, M. J., Choksi, S., and Liu, Z.G., TNF-induced activation of the Nox1 NADPH oxidase and its role in the induction of necrotic cell death. Mol. Cell, 26, 675–687(2007).
Lazzarino, D. A., de Diego, M., Hirschman, S. Z., Zhang, K. Y., Shaikh, S., Musi, E., Liaw, L., and Alexander, R. J., IL-8 and MCP-1 secretion is enhanced by the peptide-nucleic acid immunomodulator, Product R, in U937 cells and primary human monocytes. Cytokine, 14, 234–239 (2001).
Li, J. M., Fan, L. M., Christie, M. R., and Shah, A. M., Acute tumor necrosis factor alpha signaling via NADPH oxidase in microvascular endothelial cells: role of p47phox phosphorylation and binding to TRAF4. Mol. Cell Biol., 25, 2320–2330(2005).
Libby, P., Inflammation in atherosclerosis. Nature, 420, 868–874(2002).
Lusis, A. J., Atherosclerosis, Nature, 407, 233–241 (2000).
Magder, S., Neculcea, J., Neculcea, V., and Sladek, R., Lipopolysaccharide and TNF-alpha produce very similar changes in gene expression in human endothelial cells. J. Vasc. Res., 43, 447–461 (2006).
Matthews, N., Neale, M. L., Jackson, S. K., and Stark, J. M., Tumour cell killing by tumour necrosis factor: inhibition by anaerobic conditions, free-radical scavengers and inhibitors of arachidonate metabolism. Immunology, 62, 153–155 (1987).
McPhillips, K., Janssen, W. J., Ghosh, M., Byrne, A., Gardai, S., Remigio, L., Bratton, D. L., Kang, J. L., and Henson, P., TNF-alpha inhibits macrophage clearance of apoptotic cells via cytosolic phospholipase A2 and oxidant-dependent mechanisms. J. Immunol., 178, 8117–8126 (2007).
Mervaala, E. M., Muller, D. N., Park, J. K., Schmidt, F., Lohn, M., Breu, V., Dragun, D., Ganten, D., Haller, H., and Luft, F. C., Monocyte infiltration and adhesion molecules in a rat model of high human renin hypertension. Hypertension, 33, 389–395 (1999).
Miller, A. M., McPhaden, A. R., Preston, A., Wadsworth, R. M., and Wainwright, C. L., TNF alpha increases the inflammatory response to vascular balloon injury without accelerating neointimal formation. Atherosclerosis, 179, 51–59 (2005)
Neish, A. S., Williams, A. J., Palmer, H. J., Whitley, M. Z., and Collins, T., Functional analysis of the human vascular cell adhesion molecule 1 promoter. J. Exp. Med., 176, 1583–1593(1992).
Osterud, B. and Bjorklid, E., Role of monocytes in atherogenesis. Physiol. Rev., 83, 1069–1112 (2003).
Rezaie-Majd, A., Prager, G. W., Bucek, R. A., Schernthaner, G. H., Maca, T., Kress, H. G., Valent, P., Binder, B. R., Minar, E., and Baghestanian, M., Simvastatin reduces the expression of adhesion molecules in circulating monocytes from hypercholesterolemic patients. Arterioscler. Thromb. Vasc. Biol., 23, 397–403 (2003).
Rollins, B. J., Monocyte chemoattractant protein 1: a potential regulator of monocyte recruitment in inflammatory disease. Mol. Med. Today, 2, 198–204 (1996).
Ross, R., Atherosclerosis-an inflammatory disease. New Engl. J. Med., 340, 115–126 (1999).
Satoh, K., Ichihara, K., Landon, E. J., Inagami, T., and Tang, H., 3-Hydroxy-3-methylglutaryl-CoA reductase inhibitors block calcium-dependent tyrosine kinase Pyk2 activation by angiotensin II in vascular endothelial cells. involvement of geranylgeranylation of small G protein Rap1. J. Biol. Chem., 276, 15761–15767 (2001).
Sawa, Y., Sugimoto, Y., Ueki, T., Ishikawa, H., Sato, A., Nagato, T., and Yoshida, S., Effects of TNF-alpha on leukocyte adhesion molecule expressions in cultured human lymphatic endothelium. J. Histochem. Cytochem., 55, 721–733 (2007).
Schulze-Osthoff, K., Beyaert, R., Vandevoorde, V., Haegeman, G., and Fiers, W., Depletion of the mitochondrial electron transport abrogates the cytotoxic and gene-inductive effects of TNF. EMBO J., 12, 3095–3104 (1993).
Sica, A., Wang, J. M., Colotta, F., Dejana, E., Mantovani, A., Oppenheim, J. J., Larsen, C. G., Zachariae, C. O., and Matsushima, K., Monocyte chemotactic and activating factor gene expression induced in endothelial cells by IL-1 and tumor necrosis factor. J. Immunol., 144, 3034–3038 (1990).
Stokes, K. Y., Clanton, E. C., Russell, J. M., Ross, C. R., and Granger, D. N., NAD(P)H oxidase-derived superoxide mediates hypercholesterolemia-induced leukocyte-endothelial cell adhesion. Circ. Res., 88, 499–505 (2001).
Takeya, M., Yoshimura, T., Leonald, E. J., and Takahashi, K., Detection of monocyte chemoattractant protein-1 in human atherosclerotic lesions by an anti-monocyte chemoattractant protein-1 monoclonal antibody. Hum. Pathol., 24, 534–539(1993).
Thurberg, B. L. and Collins, T., The nuclear factor-kappa B/inhibitor of kappa B autoregulatory system and atherosclerosis. Curr. Opin. Lipidol., 9, 387–396 (1998).
Usui, M., Egashira, K., Tomita, H., Koyanagi, M., Katoh, M., Shimokawa, H., Takeya, M., Yoshimura, T., Matsushima, K., and Takeshita, A., Important role of local angiotensin II activity mediated via type 1 receptor in the pathogenesis of cardiovascular inflammatory changes induced by chronic blockade of nitric oxide synthesis in rats. Circulation, 101,305–310 (2000).
Vanden Berghe, T., Declercq, W., and Vandenabeele, P., NADPH oxidases: new players in TNF-induced necrotic cell death. Mol. Cell., 26, 769–771 (2007).
Veillard, N. R., Braunersreuther, V., Arnaud, C., Burger, F., Pelli, G., Steffens, S., and Mach, F., Simvastatin modulates chemokine and chemokine receptor expression by geranylgeranyl isoprenoid pathway in human endothelial cells and macrophages. Atherosclerosis, 188, 51–58 (2006).
Warnholtz, A., Nickenig, G., Schulz, E., Macharzina, R., Bräsen, J. H., Skatchkov, M., Heitzer, T., Stasch, J. P., Griendling, K. K., Harrison, D. G., Böhm, M., Meinertz, T., and Münzel, T., Increased NADH-oxidase-mediated superoxide production in the early stages of atherosclerosis: evidence for involvement of the renin-angiotensin system. Circulation, 99, 2027–2033(1999).
Weiss, D., Sorescu, D., and Taylor, W. R., Angiotensin II and atherosclerosis. Am. J. Cardiol., 87, 25C–32C (2001).
Woo, J. T., Nakagawa, H., Krecic, A. M., Nagai, K., Hamilton, A. D., Sebti, S. M., and Stern, P. H., Inhibitory effects of mevastatin and a geranylgeranyl transferase I inhibitor (GGTI-2166) on mononuclear osteoclast formation induced by receptor activator of NF kappa B ligand (RANKL) or tumor necrosis factor-alpha (TNF-alpha). Biochem. Pharmacol., 69,87–95 (2005).
Xu, Y. C., Wu, R. F., Gu, Y., Yang, Y. S., Yang, M. C., Nwariaku, F. E., and Terada, L. S., Involvement of TRAF4 in oxidative activation of c-Jun N-terminal kinase. J. Biol. Chem., 277,28051–28057 (2002).
Yang, Y. Y., Hu, C. J., Chang, S. M., Tai, T. Y., and Leu, S. J., Aspirin inhibits monocyte chemoattractant protein-1 and interleukin-8 expression in TNF-alpha stimulated human umbilical vein endothelial cells. Atherosclerosis, 174, 207–213 (2004).
Yoshimoto, K., Nishizawa, S., Koshino, H., Sato, Y., Teramae, N., and Maeda, M., Assignment of hydrogen-bond structure in a ligand-nucleobase complex inside duplex DNA: combined use of quantum chemical calculations and 15N NMR experiments. Nucleic Acids Symp. Ser (Oxf)., 49, 255–256 (2005).
Zhang, H., Schmeisser, A., Garlichs, C. D., Plotze, K., Damme, U., Mugge, A., and Daniel, W. G., Angiotensin II-induced superoxide anion generation in human vascular endothelial cells: role of membrane-bound NADH-/NADPH-oxidases. Cardiovasc. Res., 44, 215–222 (1999).
Zimmerman, R. J., Chan, A., and Leadon, S. A., Oxidative damage in murine tumor cells treated in vitro by recombinant human tumor necrosis factor. Cancer Res., 49, 1644–1648 (1989).
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Park, SY., Lee, JS., Ko, Y.J. et al. Inhibitory effect of simvastatin on the TNF-α- and angiotensin II-induced monocyte adhesion to endothelial cells is mediated through the suppression of geranylgeranyl isoprenoid-dependent ROS generation. Arch. Pharm. Res. 31, 195–204 (2008). https://doi.org/10.1007/s12272-001-1141-2
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DOI: https://doi.org/10.1007/s12272-001-1141-2