Background
Methods
Study patients
Blood sampling
Serum ACE activity measurement using spectrophotometric assay
Serum lysozyme activity measurement using radial immunodiffusion
Statistical analysis
Results
Demographics of the patient and control groups
Groups | n | Min age | Max age | Mean age | SD | 95 % CI for Mean Lower Bound | 95 % CI for Mean Upper Bound |
---|---|---|---|---|---|---|---|
AS | 76 | 15 | 74 | 41.868 | 12.536 | 37.003 | 44.733 |
BD | 72 | 9 | 75 | 38.277 | 14.681 | 34.827 | 41.727 |
P. Sarcoidosis | 31 | 10 | 72 | 41.000 | 18.954 | 34.047 | 47.952 |
TB | 68 | 21 | 86 | 52.735 | 15.525 | 48.977 | 56.493 |
P. Syphilis | 11 | 22 | 67 | 44.272 | 14.553 | 34.495 | 54.050 |
Control | 22 | 12 | 68 | 33.090 | 15.377 | 26.272 | 39.908 |
Total | 280 | 9 | 86 | 42.892 | 16.013 | 41.009 | 44.776 |
Groups | Female | Male | n |
---|---|---|---|
AS | 35 (46.1 %) | 41 (53.9 %) | 76 |
BD | 43 (59.7 %) | 29 (40.3 %) | 72 |
P. Sarcoidosis | 13 (41.9 %) | 18 (58.1 %) | 31 |
TB | 36 (52.9 %) | 32 (47.1 %) | 68 |
P. Syphilis | 4 (36.4 %) | 7 (63.6 %) | 11 |
Control | 8 (36.4 %) | 14 (63.6 %) | 22 |
Total | 139 (49.6) | 141 (50.4) | 280 |
Serum ACE and lysozyme activities of the patient and control groups
Groups | Mean value | SD | 95 % CI Lower bound | 95 % CI Upper bound |
---|---|---|---|---|
Serum ACE Level (U/L) | ||||
AS | 29.363 | 2.012 | 25.403 | 33.324 |
BD | 31.486 | 2.091 | 27.369 | 35.603 |
P. Sarcoidosis | 58.270 | 3.151 | 52.067 | 64.472 |
TB | 32.504 | 2.254 | 28.066 | 36.942 |
P. Syphilis | 30.449 | 5.285 | 20.044 | 40.854 |
Control | 21.254 | 3.800 | 13.772 | 28.736 |
Serum Lysozyme Level (mg/L) | ||||
AS | 14.124 | 0.718 | 12.710 | 15.538 |
BD | 14.371 | 0.747 | 12.901 | 15.841 |
P. Sarcoidosis | 20.765 | 1.125 | 18.550 | 22.979 |
TB | 14.987 | 0.805 | 13.402 | 16.571 |
P. Syphilis | 13.980 | 1.887 | 10.265 | 17.695 |
Control | 13.196 | 1.357 | 10.525 | 15.868 |
Pairwise comparisons of the mean differences of serum ACE levels between groups
Pairwise Comparison | Mean Difference | Standard Deviation | Sigb
| 95 % CI Lower Bound | 95 % CI Upper Bound |
---|---|---|---|---|---|
AS - BD | −2.123 | 2.894 | 0.689 | −10.691 | 6.446 |
AS - P. Sarcoidosis | −28.907 | 3.735 | 0.0001 | −39.969 | −17.845 |
AS - TB | −3.141 | 3.038 | 1.000 | −12.137 | 5.856 |
AS - P. Syphilis | −1.086 | 5.656 | 0.996 | −17.836 | 15.665 |
AS - Control | 8.110 | 4.288 | 0.895 | −4.590 | 20.809 |
BD - AS | 2.123 | 2.894 | 0.689 | −6.446 | 10.691 |
BD – P. Sarcoidosis | −26.784 | 3.770 | 0.0001 | −37.948 | −15.620 |
BD - TB | −1.018 | 3.149 | 1.000 | −10.343 | 8.306 |
BD - P. Syphilis | 1.037 | 5.689 | 0.817 | −15.812 | 17.885 |
BD - Control | 10.232 | 4.285 | 0.264 | −2.458 | 22.922 |
P. Sarcoidosis - AS | 28.907 | 3.735 | 0.0001 | 17.845 | 39.969 |
P. Sarcoidosis - BD | 26.784 | 3.770 | 0.0001 | 15.620 | 37.948 |
P. Sarcoidosis - TB | 25.766 | 3.898 | 0.0001 | 14.222 | 37.310 |
P. Sarcoidosis–P. Syphilis | 27.821 | 6.155 | 0.0001 | 9.593 | 46.048 |
P. Sarcoidosis–Control | 37.016 | 4.918 | 0.0001 | 22.453 | 51.580 |
TB - AS | 3.141 | 3.038 | 1.000 | −5.856 | 12.137 |
TB - BD | 1.018 | 3.149 | 1.000 | −8.306 | 10.343 |
TB - P. Sarcoidosis | −25.766 | 3.898 | 0.0001 | −37.310 | 14.222 |
TB - P. Syphilis | 2.055 | 5.734 | 0.504 | −14.925 | 19.095 |
TB - Control | 11.250 | 4.528 | 0.204 | −2.159 | 24.660 |
P. Syphilis - AS | 1.086 | 5.656 | 0.996 | −6.124 | 5.837 |
P. Syphilis - BD | −1.037 | 5.689 | 0.817 | −17.885 | 15.812 |
P. Syphilis–P. Sarcoidosis | −27.821 | 6.155 | 1.000 | −19.035 | 14.925 |
P. Syphilis - Control | 9.195 | 6.520 | 1.000 | −10.113 | 28.504 |
Control - AS | −8.110 | 4.288 | 0.895 | −20.809 | 4.590 |
Control - BD | −10.232 | 4.285 | 0.264 | −22.922 | 2.458 |
Control –P. Sarcoidosis | −37.016 | 4.918 | 0.0001 | −51.580 | −22.453 |
Control - TB | −11.250 | 4.528 | 0.204 | 24.660 | 2.159 |
Control - P. Syphilis | −9.195 | 6.520 | 1.000 | −28.504 | 10.113 |
Pairwise comparisons of the mean differences of serum lysozyme levels between groups
Pairwise Comparison | Mean Difference | Standard Deviation | Sig.b | 95 % CI Lower Bound | 95 % CI Upper Bound |
---|---|---|---|---|---|
AS - BD | −0.247 | 1.033 | 1.000 | −3.306 | 2.812 |
AS - P. Sarcoidosis | −6.641 | 1.334 | 0.0001 | −10.590 | −2.691 |
AS - TB | −0.863 | 1.085 | 1.000 | −4.075 | 2.349 |
AS - P. Syphilis | 0.144 | 2.020 | 1.000 | −5.837 | 6.124 |
AS - Control | 0.928 | 1.531 | 1.000 | −3.607 | 5.462 |
BD - AS | 0.247 | 1.033 | 1.000 | −2.812 | 3.306 |
BD – P. Sarcoidosis | −6.394 | 1.346 | 0.0001 | −10.380 | −2.408 |
BD - TB | −0.616 | 1.124 | 1.000 | −3.945 | 2.713 |
BD - P. Syphilis | 0.391 | 2.031 | 1.000 | −5.625 | 6.407 |
BD - Control | 1.175 | 1.530 | 1.000 | −3.356 | 5.705 |
P. Sarcoidosis - AS | 6.641 | 1.334 | 0.0001 | 2.691 | 10.590 |
P. Sarcoidosis - BD | 6.394 | 1.346 | 0.0001 | 2.408 | 10.380 |
P. Sarcoidosis - TB | 5.778 | 1.392 | 0.001 | 1.656 | 9.900 |
P. Sarcoidosis–P. Syphilis | 6.785 | 2.198 | 0.033 | 0.277 | 13.293 |
P. Sarcoidosis – Control | 7.568 | 1.756 | 0.0001 | 2.369 | 12.768 |
TB - AS | 0.863 | 1.085 | 1.000 | −2.349 | 4.075 |
TB - BD | 0.616 | 1.124 | 1.000 | −2.713 | 3.945 |
TB - P. Sarcoidosis | −5.778 | 1.392 | 0.001 | −9.900 | −1.656 |
TB - P. Syphilis | 1.007 | 2.047 | 1.000 | −5.056 | 7.069 |
TB - Control | 1.790 | 1.617 | 1.000 | −2.997 | 6.578 |
P. Syphilis - AS | −0.144 | 2.020 | 1.000 | −6.124 | 5.837 |
P. Syphilis - BD | −0.391 | 2.031 | 1.000 | −6.407 | 5.625 |
P. Syphilis – P. Sarcoidosis | −6.785 | 2.198 | 0.033 | −13.293 | −0.277 |
P. Syphilis - TB | −1.007 | 2.047 | 1.000 | −7.069 | 5.056 |
P. Syphilis - Control | 0.784 | 2.328 | 1.000 | −6.110 | 7.678 |
Control - AS | −0.928 | 1.531 | 1.000 | −5.462 | 3.607 |
Control - BD | −1.175 | 1.530 | 1.000 | −5.705 | 3.356 |
Control – P. Sarcoidosis | −7.568 | 1.756 | 0.0001 | −12.768 | −2.369 |
Control - TB | −1.790 | 1.617 | 1.000 | −6.578 | 2.997 |
Control - P. Syphilis | −0.784 | 2.328 | 1.000 | −7.678 | 6.110 |