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Generation of a Vaccine against Group B Streptococcal Infection on the Basis of a Cold-Adapted Influenza A Virus

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Abstract

The aim of this study was to develop a cold-adapted influenza A (H7N9) strain-based vector vaccine expressing epitopes of conserved lipoprotein of Streptococcus agalactiae. Recombinant hemagglutinin (HA) genes of live attenuated influenza vaccine (LAIV) strain A/Anhui/1/2013(H7N9) with insertions of group B Streptococcus (GBS) surface antigen ScaAB gene parts of different length in-frame between signal peptide and HA1 were constructed by means of reverse genetics. Viral vector vaccine H7-ScaAB-85 expressing the shortest (85 amino acids) polypeptide from lipoprotein ScaAB linked to HA via a flexible linker was genetically stable, replicated in embryonated chicken eggs and MDCK cells at the same level as cold-adapted influenza A (H7N9) strain. An increase of ScaAB polypeptide insertion length up to 141 amino acids resulted in a decrease of the viral replication level in both susceptible systems and intense mutagenesis of hybrid HA-gene of recombinant influenza A virus in the gene region encoding foreign bacterial lipoprotein. These findings suggest that H7-ScaAB-85 is a promising vaccine candidate, which should be further assessed using a mouse model.

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Funding

This study was supported by a Grant of the President of the Russian Federation for State Support of Leading Scientific Schools, project no. NSh-9646.2016.7.

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Authors and Affiliations

  1. Institute of Experimental Medicine, 197376, St. Petersburg, Russia

    T. A. Smolonogina, I. N. Isakova-Sivak, T. S. Kotomina, A. S. Evsina, E. A. Stepanova, P. I. Prokopenko, G. F. Leontieva, A. N. Suvorov & L. G. Rudenko

Authors
  1. T. A. Smolonogina
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  2. I. N. Isakova-Sivak
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  3. T. S. Kotomina
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  4. A. S. Evsina
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  6. P. I. Prokopenko
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  7. G. F. Leontieva
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Correspondence to T. A. Smolonogina.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by M. Novikova

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Smolonogina, T.A., Isakova-Sivak, I.N., Kotomina, T.S. et al. Generation of a Vaccine against Group B Streptococcal Infection on the Basis of a Cold-Adapted Influenza A Virus. Mol. Genet. Microbiol. Virol. 34, 25–34 (2019). https://doi.org/10.3103/S0891416819010087

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