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01.09.2006 | ORIGINAL CONTRIBUTION

Lupin kernel fiber consumption modifies fecal microbiota in healthy men as determined by rRNA gene fluorescent in situ hybridization

verfasst von: Stuart C. Smith, PhD, Rachel Choy, BSc Hons, Stuart K. Johnson, PhD, Ramon S. Hall, PhD, Alida C.M. Wildeboer-Veloo, MSc, Gjalt W. Welling, PhD

Erschienen in: European Journal of Nutrition | Ausgabe 6/2006

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Abstract

Background

Changes in the composition of gastrointestinal microbiota by dietary interventions using pro- and prebiotics provide opportunity for improving health and preventing disease. However, the capacity of lupin kernel fiber (LKFibre), a novel legume-derived food ingredient, to act as a prebiotic and modulate the colonic microbiota in humans needed investigation.

Aim of the study

The present study aimed to determine the effect of LKFibre on human intestinal microbiota by quantitative fluorescent in situ hybridization (FISH) analysis.

Design

A total of 18 free-living healthy males between the ages of 24 and 64 years consumed a control diet and a LKFibre diet (containing an additional 17–30 g/day fiber beyond that of the control—incorporated into daily food items) for 28 days with a 28-day washout period in a single-blind, randomized, crossover dietary intervention design.

Methods

Fecal samples were collected for 3 days towards the end of each diet and microbial populations analyzed by FISH analysis using 16S rRNA gene-based oligonucleotide probes targeting total and predominant microbial populations.

Results

Significantly higher levels of Bifidobacterium spp. (P = 0.001) and significantly lower levels of the clostridia group of C. ramosum, C. spiroforme and C. cocleatum (P = 0.039) were observed on the LKFibre diet compared with the control. No significant differences between the LKFibre and the control diet were observed for total bacteria, Lactobacillus spp., the Eubacterium spp., the C. histolyticum/C. lituseburense group and the Bacteroides–Prevotella group.

Conclusions

Ingestion of LKFibre stimulated colonic bifidobacteria growth, which suggests that this dietary fiber may be considered as a prebiotic and may beneficially contribute to colon health.

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Titel: Lupin kernel fiber consumption modifies fecal microbiota in healthy men as determined by rRNA gene fluorescent in situ hybridization
verfasst von: Stuart C. Smith, PhD
Rachel Choy, BSc Hons
Stuart K. Johnson, PhD
Ramon S. Hall, PhD
Alida C.M. Wildeboer-Veloo, MSc
Gjalt W. Welling, PhD
Publikationsdatum: 01.09.2006
Verlag: Steinkopff-Verlag
Erschienen in: European Journal of Nutrition / Ausgabe 6/2006
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-006-0603-1

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Lupin kernel fiber consumption modifies fecal microbiota in healthy men as determined by rRNA gene fluorescent in situ hybridization