Background
Research has suggested links between a mother’s immune system during pregnancy and fetal growth and brain development [
1,
2]. Central nervous system infections in pregnancy, such as herpes simplex virus and rubella, have been associated with intrauterine growth restriction, prematurity, and long-term neurodevelopmental deficits or disability [
3]. Maternal prenatal bacterial infections have been linked with lower mean IQ scores at age 7 [
4], and viral infections have shown associations with decreased IQ scores at age 7 with strong effect sizes [
5] and learning disabilities [
6]. In addition to reported effects on children’s cognition [
7] and socioemotional difficulties [
8], maternal infections have also been linked to a variety of psychiatric conditions such as bipolar disorder and schizophrenia [
9,
10]. These studies demonstrate associations between maternal infections and general difficulties in cognition, behavior, and psychopathology.
The occurrence of viral maternal infection is proposed to influence the pathogenesis of infant central nervous system abnormalities via placental transfer-mediated vertical transmission [
11]. This transmission may further disrupt the fetus’ organ and brain tissue development, negatively affecting the infant’s immune system, resulting in further pathology of a compromised immune system and increased risk of childhood inflammation [
2]. Childhood low-grade inflammation then activates a systemic immune response associated with negative effects on brain development and function, with research showing small to moderate effect sizes on general intelligence [
12]. These processes reflect multiple potential mechanisms of harm acting upon the child when maternal infection is present during pregnancy.
Even though trimester-specific associations have been reported for maternal infections and fetal neurodevelopment [
5], evidence is still lacking on whether cognitive outcomes depend on the specific timing of maternal infection exposure during gestation. The idea of fetal neurodevelopment being stage-dependent is based on the fact that brain myelination only begins during the second trimester of pregnancy with further growth reinforcements in the third trimester, followed by postnatal growth [
10,
13]. Therefore, inflammation such as infections during specific gestation periods may differentially affect the fetal growth process. However, only a limited number of studies have found that maternal inflammation during later gestational periods negatively affects a child’s cognitive-developmental infrastructure [
14] and functional connections such as working memory [
15].
Current research shows converging evidence between animal and human studies, with a review of large cohort studies suggesting a causal relationship between prenatal maternal inflammation and child outcomes [
16], particularly with effects on the fetal brain development [
17]. Examining the links between prenatal infection at different stages of gestation and cognitive outcomes is, however, subject to significant methodological challenges. In particular, it is not possible to conduct randomized experiments in humans for obvious ethical reasons. This makes it difficult to attribute causality to a factor such as prenatal infection. On the other hand, animal studies in which experimental manipulations are possible have been criticized for potential problems with generalization to humans [
18]. As such, the field must rely on triangulation across these different evidence sources with complementary strengths and weaknesses [
19‐
21].
Despite the fact that human studies must rely on observational evidence, they can provide valuable predictive information about which children may be most at risk of poorer cognitive outcomes. For example, identifying an association between prenatal infection at a particular stage of pregnancy and poorer cognitive ability can help identify children who may benefit from a higher level of monitoring and intervention early in life, such as screening for learning difficulties or prioritization for early-life preventive interventions. This study seeks to utilize observed evidence to provide predictive evidence of mother-fetal associations, despite limitations in examining specific underlying processes or mechanisms.
The aim of this study is to use a cohort sample to investigate whether the occurrence of maternal infections in each trimester of pregnancy is associated with children’s verbal, performance, and total IQ scores.
Results
Table 1
Descriptive statistics for predictors and outcomes
Maternal Prenatal Infections
| | | |
Any time during pregnancy (
n
= 6,849)
| | | |
No | 3107 | 45.4 | |
Yes | 3742 | 54.6 | |
1st trimester (
n
= 6,889)
| | | |
No | 5288 | 76.8 | |
Yes | 1601 | 23.2 | |
2nd trimester (n = 6,860) | | | |
No | 4468 | 65.1 | |
Yes | 2392 | 34.9 | |
3rd trimester (n = 7,091) | | | |
No | 5588 | 78.8 | |
Yes | 1503 | 21.2 | |
Child scores |
Mean
|
SD
|
Range
|
GMDS (Mean age = 1.5 years) | | | |
Hearing /Speech (Verbal) | 100 | 16.1 | 62–114 |
Performance | 113 | 12.7 | 64–135 |
Average | 108 | 8.8 | 65–114 |
WPPSI-RUK (Mean age = 4.1 years) |
Verbal Scale | 100 | 13.6 | 54–152 |
Performance Scale | 108 | 14.6 | 55–151 |
Fullscale | 104 | 14.2 | 52–113 |
WISC-III (Mean age = 8.6 years)
|
Verbal Scale | 107 | 16.7 | 46–155 |
Performance Scale | 100 | 17.1 | 46–151 |
Total Scale | 104 | 16.5 | 45–151 |
Table 2
Confounders and covariates (n = 7,410)
Confounders
| | |
|
Mean (SD)
|
Range
|
Maternal age at childbirth (years)
| 29.1 (1.23) | 15–44 |
|
N
|
%
|
Maternal education
| | |
(≥ A’ levels) | 3068 | 41.3 |
(< A’ levels) | 4111 | 55.5 |
Missing | 231 | 3.2 |
Deprivation index
| | |
Highest quintile | 1965 | 26.5 |
60–80% | 1162 | 15.7 |
40–60% | 1054 | 14.2 |
20–40% | 885 | 11.9 |
Lowest quintile | 765 | 10.3 |
Missing | 1579 | 21.3 |
Covariates
| | |
Child Gender
| | |
Male | 3740 | 50.4 |
Female | 3670 | 49.6 |
Child Gestation
| | |
≥ 37 weeks | 6982 | 94.2 |
< 37 weeks | 428 | 5.8 |
Child Birthweight
| | |
≥ 2500 g | 6970 | 94.0 |
< 2500 g | 349 | 4.7 |
Missing | 91 | 1.3 |
Maternal psychiatric history
| | |
No | 5955 | 80.4 |
Yes | 829 | 11.2 |
Missing | 626 | 8.4 |
Maternal smoking (any point in pregnancy)
| | |
No | 5073 | 68.5 |
Yes | 1488 | 20.1 |
Missing | 849 | 11.4 |
Table 3
Hierarchical regression models on trimester-based infections in pregnancy and cognitive outcomes (n = 7,410)
| | |
B values (
SE
)
|
Model 1
a
|
GMDS
| Hearing & Speech (verbal) | − 1.55 (.985) | − 0.429 (1.20) | − 1.32 (1.15) | − 0.940 (1.31) |
| | Performance | − 0.099 (.778) | 0.029 (.943) | − 0.191 (.901) | − 0.177 (1.03) |
| | Average | − 0.854 (.543) | − 0.647 (.655) | − 0.623 (.626) | − 0.297 (.717) |
|
WPPSI-RUK
| Verbal | − 1.45 (.887) | − 0.547 (1.07) | − 0.051 (1.02) | − 2.51 (1.16) * |
| | Performance | − 0.438 (.951) | 0.647 (1.15) | 0.430 (1.10) | − 0.810 (1.25) |
| | Fullscale | − 1.07 (.923) | 0.037 (1.11) | − 0.061 (1.07) | − 1.95 (1.21) |
|
WISC-III
| Verbal | − 0.287 (.418) | 0.945 (.509) | − 0.120 (.482) | − 1.48 (.562) ** |
| | Performance | − 0.378 (.427) | 0.449 (.517) | − 0.062 (.491) | − 1.49 (.571) ** |
| | Total | − 0.368 (.412) | 0.796 (.501) | − 0.116 (.475) | − 1.64 (.553) ** |
Model 2
b
|
GMDS
| Hearing & Speech (verbal) | − 0.150 (.148) | − 1.09 (1.38) | − 1.54 (1.33) | − 0.915 (1.55) |
| | Performance | − 0.228 (.885) | − 0.169 (1.06) | 0.078 (1.02) | − 0.606 (1.19) |
| | Average | − 0.637 (.605) | − 0.913 (.729) | − 0.313 (.706) | − 0.530 (.820) |
|
WPPSI-RUK
| Verbal | − 1.37 (.960) | − 1.14 (1.15) | − 1.42 (1.10) | − 1.75 (1.26) |
| | Performance | − 0.508 (1.04) | 0.163 (1.25) | − 0.116 (1.20) | − 0.490 (1.38) |
| | Fullscale | − 1.04 (.993) | − 0.614 (1.19) | − 0.871 (1.14) | − 1.30 (1.31) |
|
WISC-III
| Verbal | − 0.063 (.431) | 0.317 (.523) | − 0.262 (.498) | − 0.859 (.584) |
| | Performance | − 0.192 (.457) | 0.151 (.552) | 0.228 (.524) | − 1.65 (.615) ** |
| | Total | − 0.139 (.425) | 0.289 (.515) | − 0.070 (.489) | − 1.34 (.574) * |
Model 3
c
|
GMDS
| Hearing & Speech (verbal) | − 1.71 (1.22) | − 1.21 (1.44) | − 0.349 (1.39) | − 1.61 (1.64) |
| | Performance | − 0.132 (.952) | − 0.077 (1.11) | 0.396 (1.08) | − 0.315 (1.27) |
| | Average | − 0.612 (.642) | − 0.791 (.756) | 0.286 (.734) | − 0.565 (.862) |
|
WPPSI-RUK
| Verbal | − 1.08 (1.05) | 0.852 (1.22) | − 0.983 (1.17) | − 1.64 (1.36) |
| | Performance | − 0.167 (1.13) | 0.682 (1.32) | − 0.028 (1.27) | − 0.448 (1.47) |
| | Fullscale | − 0.692 (1.08) | − 0.150 (1.26) | − 0.540 (1.21) | − 1.24 (1.40) |
|
WISC-III
| Verbal | 0.304 (.467) | 0.415 (.557) | − 0.036 (.531) | − 0.603 (.623) |
| | Performance | 0.172 (.493) | 0.259 (.587) | 0.496 (.559) | − 1.41 (.655) * |
| | Total | 0.255 (.459) | 0.412 (.548) | 0.188 (.522) | − 1.34 (.574) |
Descriptive statistics showed that the occurrence of maternal prenatal infections at any time during pregnancy was 54.6%, with most infections occurring during the 2nd trimester (34.9%) (Table
1). Mean developmental quotient and intelligence quotient scores were similar to the general population (GMDS = 108, WPPSI-RUK = 104, WISC-III = 104) (Table
1). Table
2 shows further descriptive statistics for maternal variables and children’s birth outcomes.
Unadjusted models found no links between infections occurring in the first or second trimesters and children’s outcomes. Significant associations were found between third trimester infections and WPPSI-RUK verbal scores (β= − 0.076,
p = .031) at age 4, and WISC-III verbal IQ scores (β= − 0.036,
p = .008), performance IQ scores (β= − 0.036,
p = .009), and total IQ scores (β= − 0.040,
p = .003) at age 8 (Table
3). These results showed that an infection during the third trimester of pregnancy was associated with a 2.5 IQ point decrease for WPPSI-RUK verbal scores at age 4, 1.5 IQ point decrease in WISC-III verbal scores, 1.5 IQ point decrease for WISC-III performance scores, and 1.6 IQ point decrease for WISC-III total scores at age 8. No significant associations were found between prenatal infections in all three trimesters and any of the children’s cognitive outcomes (Model 1).
Significant associations were attenuated after the first adjustment for confounders maternal age, maternal education, deprivation, and infections in the other two trimesters (Model 2). Associations were no longer found for WPPSI-RUK verbal IQ scores (β= − 0.053,
p = .166) at 4 years and WISC-III verbal IQ scores (β= − 0.021,
p = .141) at 8 years. Significant associations remained between third-trimester infections and WISC-III performance IQ scores (β= − 0.039,
p = .007) and WISC-III total IQ scores (β= − 0.033,
p = .020) at 8 years (Table
3). These results showed that an infection during the third trimester of pregnancy was associated with a 1.7 IQ point decrease in WISC-III performance scores, 1.3 IQ point decrease for WISC-III total scores at age 8.
Fully adjusted models (Model 3) with confounders (maternal age, maternal education, deprivation indices, other two trimesters) and additional covariates (maternal smoking, maternal psychiatric history, gestation, birth weight, and child sex) showed that infections in the third trimester were associated with lower WISC-III performance scores at 8 years (β= − 0.033,
p = .032) (Table
3). These results represent a 1.4 IQ point decrease in WISC-III performance IQ scores.
Sensitivity analysis
A sensitivity analysis of the study models found consistent associations between infections occurring at the third trimester and outcomes for WISC-III verbal IQ scores (β= -1.33, p = .022), performance IQ scores (β= -1.19, p = .028), and total IQ scores (β= -1.39, p = .009) at age 8 in the unadjusted model. While no significant associations were shown between occurrence of infections at any trimester and cognitive outcome scores for the partially and fully adjusted models, lower maternal education (p < .05) and highest deprivation (p < .05) were generally seen to have associations with lowered cognitive scores across all trimesters.
Discussion
A prospective birth cohort was used to examine associations between maternal infections occurring at each trimester of pregnancy and children’s developmental and intelligence scores at 18 months, 4 years, and age 8. Results from unadjusted models provided evidence for maternal infections during the third trimester being associated with verbal IQ scores at age 4, and verbal, performance, and total IQ scores at age 8. The associations between third-trimester infections and cognitive outcomes were seen to be attenuated after adjustment for confounders, with significant associations seen only for performance and total IQ scores at age 8 after the first adjustment. Further adjustment with additional covariates left only significant associations for performance IQ scores at age 8. A similar prevalence of maternal infections during pregnancy was shown in this study as compared to other cohort samples [
3,
18].
Children’s cognitive outcomes appear to depend on critical periods in gestation, with evidence pointing to effects of infection limited to later gestation. Animal models have provided evidence on potential mechanisms, showing that inflammatory cytokines produced in response to infection may be linked to fetal brain development changes. Mice treated with analogues of bacterial or viral infections at different gestational periods showed different neurodevelopmental profiles of hippocampal reelin and GAD67 cell number expression in the hippocampal dorsal or ventral stratum oriens of offspring [
18]. Studies investigating infection-associated immunological events on the fetus showed early maternal inflammation to be linked with negative effects on the development of a fetus’ dopaminergic system at multiple levels such as cell distribution and connectivity [
38]. In contrast, later brain insults during pregnancy have been linked to deficits in cell organization and maturation of synapses which occur over an extended period, affecting cognitive function [
14].
While animal models provide a possible explanation as to how brain neurochemistry and structure can potentially be affected by different times of infection exposure, evidence from human studies, especially looking at specific trimester effects and cognitive outcomes, is lacking. Further, while some genetic patterns have been found in maternal intellectual ability and child IQ scores [
39], no firm conclusions can be drawn that suggests genetics fully accounts for children’s cognitive abilities. Instead, there is current growing emphasis on using the gene-environment interaction to examine the role of maternal inflammation and biological pathways that lead to fetal brain development and cognition [
40,
41].
Literature on human studies has been mixed, showing varying results on vulnerability to inflammation occurring in different gestational periods, especially on a child’s cognitive development [
10,
13,
15]. The significant associations between third trimester infection and cognitive outcomes at age 8 identified in this study suggest a possible latent effect of maternal systemic inflammation which may possibly influence cognitive development. This could be due to an interaction with unaccounted environmental factors after birth, such as lowered socioeconomic status, which could confound child development as it is also considered an inflammatory process during childhood [
42]. The link with maternal infections during later gestation shows that despite having fetal brain infrastructure in place, environmental factors such as deprivation could still affect brain structure or functional connections after birth.
This suggests that infections in late pregnancy may influence the postnatal processes of cognitive development. With differences shown in results using the developmental (GMDS) and intelligence quotients (WPPSI-RUK, WISC-III), it could be possible that effects of prenatal maternal infection are not evident in early development, with no identification of potential developmental delay as reflected in GMDS. However, results from formal IQ assessments such as the WPPSI-RUK and WISC-III could possibly be showing effects that emerge only under increased cognitive load, with cognitive IQ assessments created to assess advanced skills such as abstract reasoning or working memory.
While this study could not directly infer causality, study results are still consistent with previous research on maternal-fetal associations, where clinical evidence shows how presence of maternal inflammation during pregnancy using elevated cytokine profiles contribute to prenatal risk programming [
43,
44]. Other studies using these same maternal cytokines found negative influences on prenatal central nervous system development [
45], with animal models showing further evidence for negative neurobehavioral outcomes [
46]. In sum, research supports how prenatal programming consists of multiple variables such as occurrence and regulation of inflammation, genetic susceptibility, and environmental stressors; all of which interact in a complex manner that is still not yet fully understood in humans [
16], which could explain why study results were mixed. Nevertheless, this study contributes further evidence through the specific scope of how maternal infections during different trimesters of pregnancy could possibly affect childhood developmental and intelligence quotient outcomes.
Strengths and limitations
Main study strengths were the use of a large high-quality longitudinal design and a well-characterized sample. While cohort studies such as the ALSPAC tend to have considerable attrition due to reasons such as loss to follow-up related to socioeconomic status, it has been shown that differences in estimates when comparing full or restricted cohorts tended to be small [
47]. Similar infection rates during pregnancy and study attrition allowed for comparability with other cohort studies also looking at mother-child associations. Study limitations include a reliance on mother self-reported infection data; that the records of infections were not classified by severity; and that causes of infections (e.g. bacterial/viral/fungal) were not recorded and could not be stratified by pathogen type for analysis. In addition, while the study has included a list of confounders and covariates, not all factors such as parental attitudes, styles or other variables related to the quality of care of the child, or genetic factors could be accounted for in this study. In addition, this version of GMDS was created to identify developmental delays and therefore should be interpreted with caution as to whether it is a true assessment of cognition at 18 months. Despite not being able to account for these above factors, study results still showed predictive associations for the cognitive measures assessing IQ, which was consistent with research from both animal and human studies focusing on maternal-fetal interactions. Finally, assessments from the dataset were only measured up to age 8, so lasting effects of cognitive development could not be analyzed beyond those years.
Acknowledgements
We are extremely grateful to all the families who took part in this study, the midwives for their help in recruiting them, and the whole ALSPAC team, which includes interviewers, computer and laboratory technicians, clerical workers, research scientists, volunteers, managers, receptionists and nurse. We further thank the European Union’s Horizon 2020 research and innovation program for their generous support.
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