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01.08.2014 | Clinical Article - Brain Tumors

Molecular stereotactic biopsy technique improves diagnostic accuracy and enables personalized treatment strategies in glioma patients

verfasst von: Sabina Eigenbrod, Raimund Trabold, David Brucker, Christian Erös, Rupert Egensperger, Christian La Fougere, Werner Göbel, Adrian Rühm, Hans A. Kretzschmar, Jörg C. Tonn, Jochen Herms, Armin Giese, Friedrich W. Kreth

Erschienen in: Acta Neurochirurgica | Ausgabe 8/2014

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Abstract

Background

In gliomas molecular biomarkers are increasingly gaining diagnostic, prognostic and predictive significance. Determination of biomarker status after biopsy is important as not all patients are eligible for open tumor resection. We developed and validated prospectively (6/10–12/11) a protocol allowing for both reliable determination of multiple biomarkers and representative histological diagnoses from small-sized biopsies.

Methods

All molecular stereotactic biopsies were performed according to a detailed workflow. The selection of specimens best suited for molecular analyses was intra-operatively guided by the attending neuropathologist. Postoperative screening was done by methylation specific PCR using two distinct cryopreserved specimens to test for reproducibility of the findings and to rule out contamination. The DNA of a single best-suited specimen (1 mm³) was subjected to detailed molecular analysis (MGMT promoter methylation, IDH1/2 mutational status, LOH 1p and/or 19q).

Results

159 consecutively enrolled untreated gliomas were analyzed (94 glioblastomas, 2 gliosarcomas, 24 anaplastic astrocytomas, 10 oligo-tumors grade II/III, 20 grade II astrocytomas and 9 pilocytic astrocytomas). Transient morbidity was 2 %. Overall, the drop-out rate due to tissue contamination was 0.4 %. Median time from biopsy to histological and molecular genetic analyses was 3 and 5 days, respectively. Distributions of the respective biomarker status for tumor subgroups were consistent with the literature. The final histological diagnosis was changed/modified in 5/159 patients according to molecular findings. Treatment after molecular biopsy was highly personalized.

Conclusions

Molecular stereotactic biopsy is feasible and safe, can be implemented in daily clinical practice, improves diagnostic precision and enables personalized treatment.

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Titel: Molecular stereotactic biopsy technique improves diagnostic accuracy and enables personalized treatment strategies in glioma patients
verfasst von: Sabina Eigenbrod
Raimund Trabold
David Brucker
Christian Erös
Rupert Egensperger
Christian La Fougere
Werner Göbel
Adrian Rühm
Hans A. Kretzschmar
Jörg C. Tonn
Jochen Herms
Armin Giese
Friedrich W. Kreth
Publikationsdatum: 01.08.2014
Verlag: Springer Vienna
Erschienen in: Acta Neurochirurgica / Ausgabe 8/2014
Print ISSN: 0001-6268
Elektronische ISSN: 0942-0940
DOI: https://doi.org/10.1007/s00701-014-2073-1

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