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28.09.2015 | Original Paper

Monocyte–platelet aggregates and CD11b expression as markers for thrombogenicity in atrial fibrillation

verfasst von: Christian Pfluecke, Daniel Tarnowski, Lina Plichta, Katharina Berndt, Paul Schumacher, Stefan Ulbrich, Mathias Forkmann, Marian Christoph, David M. Poitz, Carsten Wunderlich, Ruth H. Strasser, Karim Ibrahim

Erschienen in: Clinical Research in Cardiology | Ausgabe 4/2016

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Abstract

Background

A strong interdependence is known between atrial fibrillation (AF), inflammation and thrombogenesis. Monocyte–platelet aggregates (MPAs) are sensitive markers of platelets and monocyte activation. It is not known whether MPAs are associated with thrombogenicity in AF. Therefore, we examined differences in the content of MPAs and CD11b expression in patients with AF in dependence of the presence of atrial thrombus formation.

Methods

107 patients with symptomatic AF underwent transesophageal echocardiography (TEE) before planned cardioversion or pulmonary vein isolation. Flow-cytometric quantification analysis was done on the day of performed TEE to determine the content of MPAs and the expression of CD11b on monocytes and granulocytes.

Results

Compared to patients without thrombus (n = 80) those with an echocardiographic proven left atrium (LA) thrombus (n = 27) showed an increased extent of the risk factors age, diabetes and heart failure. The content of MPAs (147 ± 12 vs. 311 ± 29 cells/µl, p < 0.001) as well as the CD11b expression on monocytes (p < 0.05) and granulocytes (p < 0.05) were strongly associated with the existence of a LA thrombus. The content of MPAs and the CD11b expression remained independent predictors for LA thrombus after adjustment in logistic regression analysis and negatively correlated with left atrial appendage flow velocity. MPAs above 170 cells/µl (OR 34.2, p = 0.01) had a sensitivity of 96 % and a specificity of 73 % for predicting LA-thrombus.

Conclusions

The content of MPAs and the CD11b expression on monocytes and granulocytes are increased in AF-patients with proven thrombus formation. They seem to be appropriate biomarkers for stratification of thromboembolic risk in patients with AF.

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Titel: Monocyte–platelet aggregates and CD11b expression as markers for thrombogenicity in atrial fibrillation
verfasst von: Christian Pfluecke
Daniel Tarnowski
Lina Plichta
Katharina Berndt
Paul Schumacher
Stefan Ulbrich
Mathias Forkmann
Marian Christoph
David M. Poitz
Carsten Wunderlich
Ruth H. Strasser
Karim Ibrahim
Publikationsdatum: 28.09.2015
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Research in Cardiology / Ausgabe 4/2016
Print ISSN: 1861-0684
Elektronische ISSN: 1861-0692
DOI: https://doi.org/10.1007/s00392-015-0922-4

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Springer Medizin

Monocyte–platelet aggregates and CD11b expression as markers for thrombogenicity in atrial fibrillation

Abstract

Background

Methods

Results

Conclusions

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Springer Medizin

Abstract

Background

Methods

Results

Conclusions

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