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Knee Surgery, Sports Traumatology, Arthroscopy

Erschienen in:

01.05.2015 | Experimental Study

PVA-chitosan composite hydrogel versus alginate beads as a potential mesenchymal stem cell carrier for the treatment of focal cartilage defects

verfasst von: Havva Dashtdar, Malliga Raman Murali, Azlina Amir Abbas, Abdulrazzaq Mahmod Suhaeb, Lakshmi Selvaratnam, Liang Xin Tay, Tunku Kamarul

Erschienen in: Knee Surgery, Sports Traumatology, Arthroscopy | Ausgabe 5/2015

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Abstract

Purpose

To investigate whether mesenchymal stem cells (MSCs) seeded in novel polyvinyl alcohol (PVA)-chitosan composite hydrogel can provide comparable or even further improve cartilage repair outcomes as compared to previously established alginate-transplanted models.

Methods

Medial femoral condyle defect was created in both knees of twenty-four mature New Zealand white rabbits, and the animals were divided into four groups containing six animals each. After 3 weeks, the right knees were transplanted with PVA-chitosan-MSC, PVA-chitosan scaffold alone, alginate-MSC construct or alginate alone. The left knee was kept as untreated control. Animals were killed at the end of 6 months after transplantation, and the cartilage repair was assessed through Brittberg morphological score, histological grading by O’Driscoll score and quantitative glycosaminoglycan analysis.

Results

Morphological and histological analyses showed significant (p < 0.05) tissue repair when treated with PVA-chitosan-MSC or alginate MSC as compared to the scaffold only and untreated control. In addition, safranin O staining and the glycosaminoglycan (GAG) content were significantly higher (p < 0.05) in MSC treatment groups than in scaffold-only or untreated control group. No significant difference was observed between the PVA-chitosan-MSC- and alginate-MSC-treated groups.

Conclusion

PVA-chitosan hydrogel seeded with mesenchymal stem cells provides comparable treatment outcomes to that of previously established alginate-MSC construct implantation. This study supports the potential use of PVA-chitosan hydrogel seeded with MSCs for clinical use in cartilage repair such as traumatic injuries.

Ab-Rahim S, Selvaratnam L, Raghavendran HR, Kamarul T (2013) Chondrocyte-alginate constructs with or without TGF-β1 produces superior extracellular matrix expression than monolayer cultures. Mol Cell Biochem 376:11–20CrossRefPubMed

Ahearne M, Kelly DJ (2013) A comparison of fibrin, agarose and gellan gum hydrogels as carriers of stem cells and growth factor delivery microspheres for cartilage regeneration. Biomed Mater 8:035004CrossRefPubMed

Caplan AI, Dennis JE (2006) Mesenchymal stem cells as trophic mediators. J Cell Biochem 98:1076–1084CrossRefPubMed

Charlton DC, Peterson MG, Spiller K, Lowman A, Torzilli PA, Maher SA (2008) Semi-degradable scaffold for articular cartilage replacement. Tissue Eng Part A 14:207–213CrossRefPubMedCentralPubMed

Christensen BB, Foldager CB, Hansen OM, Kristiansen AA, Le DQ, Nielsen AD, Nygaard JV, Bünger CE, Lind M (2012) A novel nano-structured porous polycaprolactone scaffold improves hyaline cartilage repair in a rabbit model compared to a collagen type I/III scaffold: in vitro and in vivo studies. Knee Surg Sports Traumatol Arthrosc 20:1192–1204CrossRefPubMed

Chuang WY, Young TH, Yao CH, Chiu WY (2008) Properties of the poly(vinyl alcohol)/chitosan blend and its effect on the culture of fibroblast in vitro. Biomaterials 20:1479–1487CrossRef

Chung C, Burdick JA (2008) Engineering cartilage tissue. Adv Drug Deliv Rev 60:243–262CrossRefPubMedCentralPubMed

Dashtdar H, Rothan HA, Tay T, Ahmad RE, Ali R, Tay LX, Chong PP, Kamarul T (2011) A preliminary study comparing the use of allogenic chondrogenic pre-differentiated and undifferentiated mesenchymal stem cells for the repair of full thickness articular cartilage defects in rabbits. J Orthop Res 29:1336–1342CrossRefPubMed

Festing MFW (1997) Variation and its implications for the design of experiments in toxicological research. Comp Haematol Int. 7:202–207CrossRef

10.

Figueroa D, Espinosa M, Calvo R, Scheu M, Vaisman A, Gallegos M, Conget P (2013) Anterior cruciate ligament regeneration using mesenchymal stem cells and collagen type I scaffold in a rabbit model. Knee Surg Sports Traumatol Arthrosc 1–7

11.

Francis NL, Hunger PM, Donius AE, Riblett BW, Zavaliangos A, Wegst UG, Wheatley MA (2013) An ice-templated, linearly aligned chitosan-alginate scaffold for neural tissue engineering. J Biomed Mater Res A. doi:10.1002/jbm.a.34668 PubMed

12.

Gao L, McBeath R, Chen CS (2010) Stem cell shape regulates a chondrogenic versus myogenic fate through Rac1 and N-cadherin. Stem Cells 28:564–572PubMedCentralPubMed

13.

Gille J, Kunow J, Boisch L, Behrens P, Bos I, Hoffmann C, Koller W, Russlier M, Kurz B (2010) Cell-laden and cell-free matrix-induced chondrogenesis versus microfracture for the treatment of articular cartilage defects a histological and biomechanical study in sheep. Cartilage 1:29–42CrossRef

14.

Gomoll AH, Madry H, Knutsen G, van Dijk N, Seil R, Brittberg M, Kon E (2010) The subchondral bone in articular cartilage repair: current problems in the surgical management. Knee Surg Sports Traumatol Arthrosc 18:434–447CrossRefPubMedCentralPubMed

15.

Gomoll AH, Filardo G, de Girolamo L, Espregueira-Mendes J, Marcacci M, Rodkey WG, Steadman JR, Zaffagnini S, Kon E (2012) Surgical treatment for early osteoarthritis. Part I: cartilage repair procedures. Knee Surg Sports Traumatol Arthrosc 20:450–466CrossRefPubMed

16.

Mansur HS, Costa HS (2008) Nanostructured poly(vinyl alcohol)/bioactive glass and poly(vinyl alcohol)/chitosan/bioactive glass hybrid scaffolds for biomedical applications. Chem Eng J 137:72–83CrossRef

17.

Hong HJ, Lee JS, Choi JW, Min BH, Lee HB, Kim CH (2012) Transplantation of autologous chondrocytes seeded on a fibrin/hyaluronan composite gel into tracheal cartilage defects in rabbits: preliminary results. Artif Organs 36:998–1006CrossRefPubMed

18.

Hunziker EB (2002) Articular cartilage repair: basic science and clinical progress. A review of the current status and prospects. Osteoarthr Cartil 10:432–463CrossRefPubMed

19.

Iyer SS, Rojas M (2008) Anti-inflammatory effects of mesenchymal stem cells: novel concept for future therapies. Expert Opin Biol Ther 8:569–581CrossRefPubMed

20.

Jang KM, Lee JH, Park CM, Song HR, Wang JH (2013) Xenotransplantation of human mesenchymal stem cells for repair of osteochondral defects in rabbits using osteochondral biphasic composite constructs. Knee Surg Sports Traumatol Arthrosc 1–11 doi:10.1007/s00167-013-2426-y

21.

Ku Y, Shim IK, Lee JY, Park YJ, Rhee SH, Nam SH, Park JB, Chung CP, Lee SJ (2009) Chitosan/poly(L-lactic acid) multilayered membrane for guided tissue regeneration. J Biomed Mater Res A 90:766–772CrossRefPubMed

22.

Lao L, Tan H, Wang Y, Gao C (2008) Chitosan modified poly(L-lactide) microspheres as cell microcarriers for cartilage tissue engineering. Colloids Surf B Biointerfaces 66:218–225CrossRefPubMed

23.

Lee SY, Pereira BP, Yusof N, Selvaratnam L, Yu Z, Abbas AA, Kamarul T (2009) Unconfined compression properties of a porous poly(vinyl alcohol)-chitosan-based hydrogel after hydration. Acta Biomater 5:1919–1925CrossRefPubMed

24.

Liao H, Qi R, Shen M, Cao X, Guo R, Zhang Y, Shi X (2011) Improved cellular response on multiwalled carbon nanotube-incorporated electrospun polyvinyl alcohol/chitosan nanofibrous scaffolds. Colloids Surf B Biointerfaces 84:528–535CrossRefPubMed

25.

Lin LC, Chang SJ, Lin CY, Lin YT, Chuang CW, Yao CH, Kuo SM (2012) Repair of chondral defects with allogenous chondrocyte-seeded hyaluronan/collagen II microspheres in a rabbit model. Artif Organs 36:E102–E109CrossRefPubMed

26.

Loken S, Jakobsen RB, Arøen A, Heir S, Shahdadfar A, Brinchmann JE, Engebretsen L, Reinholt FP (2008) Bone marrow mesenchymal stem cells in a hyaluronan scaffold for treatment of an osteochondral defect in a rabbit model. Knee Surg Sports Traumatol Arthrosc 16:896–903CrossRefPubMed

27.

Mathews S, Bhonde R, Gupta PK, Totey S (2011) A novel tripolymer coating demonstrating the synergistic effect of chitosan, collagen type 1 and hyaluronic acid on osteogenic differentiation of human bone marrow derived mesenchymal stem cells. Biochem Biophys Res Commun 414:270–276CrossRefPubMed

28.

Mawad D, Martens PJ, Odell RA, Poole-Warren LA (2007) The effect of redox polymerisation on degradation and cell responses to poly (vinyl alcohol) hydrogels. Biomaterials 28:947–955CrossRefPubMed

29.

Medina JG, Steinke JW, Das S (2012) A chitosan-based sinus sealant for reduction of adhesion formation in rabbit and sheep models. Otolaryngol Head Neck Surg 147:357–363CrossRefPubMedCentralPubMed

30.

Morais DS, Rodrigues MA, Silva TI, Lopes MA, Santos M, Santos JD, Botelho CM (2013) Development and characterization of novel alginate-based hydrogels as vehicles for bone substitutes. Carbohydr Polym 95:134–142CrossRefPubMed

31.

Nacer Khodja A, Mahlous M, Tahtat D, Benamer S, Larbi Youcef S, Chader H, Mouhoub L, Sedgelmaci M, Ammi N, Mansouri MB, Mameri S (2013) Evaluation of healing activity of PVA/chitosan hydrogels on deep second degree burn: pharmacological and toxicological tests. Burns 39:98–104CrossRefPubMed

32.

Oh JY, Kim MK, Shin MS, Lee HJ, Ko JH, Wee WR, Lee JH (2008) The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury. Stem Cells 26:1047–1055CrossRefPubMed

33.

Park H, Choi B, Hu J, Lee M (2013) Injectable chitosan hyaluronic acid hydrogels for cartilage tissue engineering. Acta Biomater 9:4779–4786CrossRefPubMed

34.

Peterson L, Minas T, Brittberg M, Nilsson A, Sjögren-Jansson E, Lindahl A (2000) Two- to 9-year outcome after autologous chondrocyte transplantation of the knee. Clin Orthop Relat Res 374:212–234CrossRefPubMed

35.

Popa E, Reis R, Gomes M (2012) Chondrogenic phenotype of different cells encapsulated in κ-carrageenan hydrogels for cartilage regeneration strategies. Biotechnol Appl Biochem 59:132–141CrossRefPubMed

36.

Qi Y, Du Y, Li W, Dai X, Zhao T, Yan W (2012) Cartilage repair using mesenchymal stem cell (MSC) sheet and MSCs-loaded bilayer PLGA scaffold in a rabbit model. Knee Surg Sports Traumatol Arthrosc. doi:10.1007/s00167-012-2256-3

37.

Raghunath J, Salacinski HJ, Sales KM, Butler PE, Seifalian AM (2005) Advancing cartilage tissue engineering: the application of stem cell technology. Curr Opin Biotechnol 16:503–509CrossRefPubMed

38.

Saris DB, Vanlauwe J, Victor J, Haspl M, Bohnsack M, Fortems Y, Vandekerckhove B, Almqvist KF, Claes T, Handelberg F, Lagae K, van der Bauwhede J, Vandenneucker H, Yang KG, Jelic M, Verdonk R, Veulemans N, Bellemans J, Luyten FP (2008) Characterized chondrocyte implantation results in better structural repair when treating symptomatic cartilage defects of the knee in a randomized controlled trial versus microfracture. Am J Sports Med 36:235–246CrossRefPubMed

39.

Schagemann JC, Erggelet C, Chung HW, Lahm A, Kurz H, Mrosek EH (2009) Cell-laden and cell-free biopolymer hydrogel for the treatment of osteochondral defects in a sheep model. Tissue Eng Part A 15:75–82CrossRefPubMed

40.

Seo SS, Kim CW, Jung DW (2011) Management of focal chondral lesion in the knee joint. Knee Surg Relat Res 23:185–196CrossRefPubMedCentralPubMed

41.

Sung JH, Hwang MR, Kim JO, Lee JH, Kim YI, Kim JH, Chang SW, Jin SG, Kim JA, Lyoo WS, Han SS, Ku SK, Yong CS, Choi HG (2010) Gel characterisation and in vivo evaluation of minocycline-loaded wound dressing with enhanced wound healing using polyvinyl alcohol and chitosan. Int J Pharm 392:232–240CrossRefPubMed

42.

Venkatesan J, Pallela R, Bhatnagar I, Kim SK (2012) Chitosan-amylopectin/hydroxyapatite and chitosan-chondroitin sulphate/hydroxyapatite composite scaffolds for bone tissue engineering. Int J Biol Macromo l51: 1033–1042

43.

Wu Y, Huang S, Enhe J, Ma K, Yang S, Sun T, Fu X (2013) Bone marrow-derived mesenchymal stem cell attenuates skin fibrosis development in mice. Int Wound J. doi:10.1111/iwj.12034

44.

Zhao Q, Wang S, Tian J, Wang L, Dong S, Xia T, Wu Z (2013) Combination of bone marrow concentrate and PGA scaffolds enhance bone marrow stimulation in rabbit articular cartilage repair. J Mater Sci Mater Med 24:793–801CrossRefPubMed

Titel: PVA-chitosan composite hydrogel versus alginate beads as a potential mesenchymal stem cell carrier for the treatment of focal cartilage defects
verfasst von: Havva Dashtdar
Malliga Raman Murali
Azlina Amir Abbas
Abdulrazzaq Mahmod Suhaeb
Lakshmi Selvaratnam
Liang Xin Tay
Tunku Kamarul
Publikationsdatum: 01.05.2015
Verlag: Springer Berlin Heidelberg
Erschienen in: Knee Surgery, Sports Traumatology, Arthroscopy / Ausgabe 5/2015
Print ISSN: 0942-2056
Elektronische ISSN: 1433-7347
DOI: https://doi.org/10.1007/s00167-013-2723-5

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PVA-chitosan composite hydrogel versus alginate beads as a potential mesenchymal stem cell carrier for the treatment of focal cartilage defects