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Journal of Clinical Immunology

Erschienen in:

01.11.2009

Specific Immunotherapy to Birch Allergen Does not Enhance Suppression of Th2 Cells by CD4⁺CD25⁺ Regulatory T Cells During Pollen Season

verfasst von: Hanna Grindebacke, Pia Larsson, Kajsa Wing, Sabina Rak, Anna Rudin

Erschienen in: Journal of Clinical Immunology | Ausgabe 6/2009

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Abstract

Introduction

The aim of this study was to investigate the suppressive capacity of CD25⁺ regulatory T cells on birch allergen-induced T-cell responses during the first birch pollen season after initiation of specific immunotherapy (SIT).

Methods

CD25^pos and CD25^neg T cells were purified from blood of birch-allergic SIT patients and birch-allergic controls, stimulated with birch pollen extract, and analyzed for T-cell proliferation and production of interferon gamma (IFN-γ), interleukin (IL)-5 and IL-10.

Results

We show that allergen-induced proliferation and IFN-γ production were suppressed equally well by CD25^pos T cells from SIT patients and controls, while the IL-5 production was not suppressed by either of the groups. IL-10 levels were higher in SIT patients relative to controls only when CD25^neg and CD25^pos were cultured together. Furthermore, neither FOXP3 levels nor proportions of CD25^high T cells were enhanced in SIT patients compared to allergic controls.

Discussion

These results suggest that the Th2-suppressive capacity of allergen-stimulated CD25^pos Treg in vitro is not improved by SIT in spite of increased IL-10 production from T cells.

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Titel: Specific Immunotherapy to Birch Allergen Does not Enhance Suppression of Th2 Cells by CD4+CD25+ Regulatory T Cells During Pollen Season
verfasst von: Hanna Grindebacke
Pia Larsson
Kajsa Wing
Sabina Rak
Anna Rudin
Publikationsdatum: 01.11.2009
Verlag: Springer US
Erschienen in: Journal of Clinical Immunology / Ausgabe 6/2009
Print ISSN: 0271-9142
Elektronische ISSN: 1573-2592
DOI: https://doi.org/10.1007/s10875-009-9312-x

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Abstract

Introduction

Methods

Results

Discussion

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