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20.05.2020 | Original Contribution

Synergetic responses of intestinal microbiota and epithelium to dietary inulin supplementation in pigs

verfasst von: Jun He, Hongmei Xie, Daiwen Chen, Bing Yu, Zhiqing Huang, Xiangbing Mao, Ping Zheng, Yuheng Luo, Jie Yu, Junqiu Luo, Hui Yan

Erschienen in: European Journal of Nutrition | Ausgabe 2/2021

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Abstract

Purpose

Inulin is a soluble dietary fiber that has been implicated in regulating the intestinal health. Here, we describe a synergetic response of intestinal microbiota and epithelial functions to increased intake of inulin in a porcine model.

Methods

Twenty growing-pigs were randomly allocated to two groups (n = 10) and fed with a basal diet (BD) or BD containing 0.5% inulin (INU) for 21 days.

Results

We show that INU supplementation not only elevated villus height and the abundance of zonula occludens-1 (ZO-1), but also increased acetate and butyrate concentrations in cecum (P < 0.05). Moreover, INU decreased IL-6 and TNFα secretion, and reduced intestinal epithelial cell apoptosis in ileum and cecum (P < 0.05). Interestingly, we observed an elevated 16S rRNA gene copies in cecum after INU ingestion (P < 0.05). INU had no influence on overall diversity, but acutely altered the abundance of specific bacteria. INU decreased the abundance of phylum Proteobacteria in ileum, but increased the phylum Bacteroidetes in the ileum and cecum (P < 0.05). INU significantly elevated the Lactobacillus spp. and Bacteroides spp. in the ileum and cecum, respectively. Importantly, INU elevated the expression levels of GPR43, GLP-2, and ZO-1, but decreased the expression levels of histone deacetylase 1 (HDAC1) and TNFα in the ileum and cecum mucosa (P < 0.05). Moreover, INU also elevated the expression levels of GPR109A and angiopoietin-4 (ANG-4) in the cecum mucosa (P < 0.05).

Conclusions

This study indicated how the intestinal microbiome and epithelium adapt to inulin ingestion, and furthered our understanding of the mechanisms behind the dietary fiber-modulated intestinal microbiota and health.

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Titel: Synergetic responses of intestinal microbiota and epithelium to dietary inulin supplementation in pigs
verfasst von: Jun He
Hongmei Xie
Daiwen Chen
Bing Yu
Zhiqing Huang
Xiangbing Mao
Ping Zheng
Yuheng Luo
Jie Yu
Junqiu Luo
Hui Yan
Publikationsdatum: 20.05.2020
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Nutrition / Ausgabe 2/2021
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-020-02284-3

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Synergetic responses of intestinal microbiota and epithelium to dietary inulin supplementation in pigs