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European Journal of Nutrition

Erschienen in:

17.08.2020 | Original Contribution

Tissue-specific effect of colitis on protein synthesis in mice: impact of the dietary protein content

verfasst von: Sandra Vidal-Lletjós, Nadezda V. Khodorova, Maria Piscuc, Claire Gaudichon, François Blachier, Annaïg Lan

Erschienen in: European Journal of Nutrition | Ausgabe 3/2021

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Abstract

Purpose

Inflammatory bowel diseases are associated with an increase in the whole-body protein turnover, thus possibly requiring an additional supply of dietary proteins. Our aim was to evaluate whether increasing dietary protein content could alleviate protein metabolism alterations in the injured splanchnic and peripheral tissues during colitis and spontaneous mucosal healing.

Methods

Mice with acute chemically induced colitis received either a normal protein (P14, 14% as energy), a moderately (P30, 30%) and a very high-protein (P53, 55%) diets. At different times after the challenge, protein synthesis rate was determined in tissues using a flooding dose of ¹³C valine.

Results

Colon, liver and spleen protein synthesis rates were significantly increased after colitis induction, while being decreased in the caecum, kidneys and muscle. Contrastingly to the two other diets, P30 diet consumption allowed faster recovery of the animals, and this coincided with a rapid resaturation of the initial protein synthesis in the colon. In the other tissues studied, the high-protein diets show different effects depending on the dietary protein content consumed and on the examined tissues, with a general trend of P53 in lowering anabolism rates.

Conclusion

This study highlights the severe impact of acute colonic inflammation on protein metabolism in different organs. In addition, dietary protein content modulated the recovery of the initial protein synthesis rate in the various tissues following colitis induction. P30 diet consumption notably showed a better ability to alleviate protein metabolism perturbations induced by colitis, that may explain its documented beneficial effect on colon mucosal healing.

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Titel: Tissue-specific effect of colitis on protein synthesis in mice: impact of the dietary protein content
verfasst von: Sandra Vidal-Lletjós
Nadezda V. Khodorova
Maria Piscuc
Claire Gaudichon
François Blachier
Annaïg Lan
Publikationsdatum: 17.08.2020
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Nutrition / Ausgabe 3/2021
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-020-02365-3

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Tissue-specific effect of colitis on protein synthesis in mice: impact of the dietary protein content