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Diabetologia

Erschienen in:

01.08.2005 | Article

Wheat protein-induced proinflammatory T helper 1 bias in mesenteric lymph nodes of young diabetes-prone rats

verfasst von: H. Chakir, D. E. Lefebvre, H. Wang, E. Caraher, F. W. Scott

Erschienen in: Diabetologia | Ausgabe 8/2005

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Abstract

Aims/hypothesis

Type 1 diabetes is the result of an inflammatory T helper 1 (Th1) lymphocyte-mediated beta cell destructive process. The majority of diabetes-prone BioBreeding (BBdp) rats fed wheat protein-based diets, such as NTP-2000, develop type 1 diabetes and display a mild coeliac-like enteropathy. Mesenteric lymph nodes (MLNs), which drain the gut, are the major inductive site where dietary antigens are recognised in the gut-associated lymphoid tissue (GALT). We hypothesised that this compartment could be a site of abnormal wheat protein-induced Th1 cell activation.

Methods

MLN cells were isolated from BBdp and BB control (BBc) rats that were fed NTP-2000 or a hydrolysed casein (HC)-based diet at ages that pre-date classic insulitis. The inflammatory status, phenotype and proliferation of these cells in response to wheat protein were determined.

Results

The expression ratio of T-bet : Gata3, master transcription factors for Th1 and Th2 cytokines, was increased in the MLN from NTP-2000-fed BBdp rats compared with that from BBc rats, mainly due to decreased Gata3 expression. CD3⁺CD4⁺IFN-γ⁺ T cells were more prevalent in the MLN of wheat-fed BBdp rats, but remained at control levels in BBdp rats fed a diabetes-retardant HC diet. BBdp MLN cells proliferated in response to wheat protein antigens in a specific, dose-dependent manner, and >93% of cells were CD3⁺CD4⁺ T cells. This proliferation was associated with a low proportion of CD4⁺CD25⁺ T cells and a high proportion of dendritic cells in the MLN of BBdp rats.

Conclusions/interpretation

Before insulitis is established, the MLNs of wheat-fed BBdp rats contain an unusually high proportion of Th1 cells that proliferate specifically in response to wheat protein antigens.

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Titel: Wheat protein-induced proinflammatory T helper 1 bias in mesenteric lymph nodes of young diabetes-prone rats
verfasst von: H. Chakir
D. E. Lefebvre
H. Wang
E. Caraher
F. W. Scott
Publikationsdatum: 01.08.2005
Verlag: Springer-Verlag
Erschienen in: Diabetologia / Ausgabe 8/2005
Print ISSN: 0012-186X
Elektronische ISSN: 1432-0428
DOI: https://doi.org/10.1007/s00125-005-1842-z

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