Overview
- Editors:
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Nicole I. Nieden
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Dept. of Cell Biology & Neuroscience, University of California at Riverside, Riverside, USA
- Contains detailed, expert protocols specific to ESCs and stem cell differentiation
- Gives special care to address possible methodological pitfalls and provides trouble-shooting guides in the form of Notes sections
- Features contributions from renowned researchers in the field of stem cell self-renewal and the pioneers in skeletogenesis from ESCs
- Includes supplementary material: sn.pub/extras
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Table of contents (21 protocols)
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- Michael D. O’Connor, Melanie D. Kardel, Connie J. Eaves
Pages 67-80
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- Helen Spencer, Maria Keramari, Christopher M. Ward
Pages 81-94
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- Parinya Noisa, Alai Urrutikoetxea-Uriguen, Wei Cui
Pages 95-106
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- Stephanie N. Rushing, Anthony W. Herren, Deborah K. Lieu, Ronald A. Li
Pages 107-120
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- Ana Fernandes-Platzgummer, Maria Margarida Diogo, Cláudia Lobato da Silva, Joaquim M. S. Cabral
Pages 121-134
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- Susanne Trettner, Alexander Seeliger, Nicole I. zur Nieden
Pages 135-149
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- Dawn P. Spelke, Daniel Ortmann, Ali Khademhosseini, Lino Ferreira, Jeffrey M. Karp
Pages 151-162
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- Andre Choo, Sai Kiang Lim
Pages 175-182
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- Emmanuel N. Olivier, Eric E. Bouhassira
Pages 183-193
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- Beatrice Kuske, Vuk Savkovic, Nicole I. zur Nieden
Pages 195-215
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- Núria Marí-Buyé, Carlos E. Semino
Pages 217-237
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- Motokazu Tsuneto, Toshiyuki Yamane, Shin-Ichi Hayashi
Pages 239-253
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- Lesley A. Davis, Anke Dienelt, Nicole I. zur Nieden
Pages 255-272
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- Nobuyuki Udagawa, Teruhito Yamashita, Yasuhiro Kobayashi, Naoyuki Takahashi
Pages 273-284
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- Boyangzi Li, Haiying Liu, Zhenqing Zhang, Hope E. Stansfield, Jonathan S. Dordick, Robert J. Linhardt
Pages 285-300
About this book
Embryonic stem cells (ESCs) offer an unlimited self-renewing capacity, as opposed to the limits of adult stem cells; therefore, ESCs represent an almost bottomless resource for regenerative medicine and tissue engineering approaches. In Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols, accomplished investigators provide detailed descriptions on how to expand ESCs from the most commonly used species ex vivo, i.e. mouse and human, in static culture as well as in controllable bioreactor processes. The thorough and timely volume summarizes the methods that may be used to differentiate these cells along the desired lineage of choice, be it osteoblasts, osteoclasts, or chondrocytes, and consequentially also offers analysis tools for the characterization of resulting cells and evaluation of differentiation effectiveness. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.
Dependable and cutting-edge, Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols supplies the tools necessary to allow researchers to carry out critical research needed in order to bring this burgeoning and vitally important field closer to the clinic and to ensure the widespread application of a successful strategy.
Reviews
From the reviews:
“Provides step-by-step protocols for the differentiation of ESCs along the musculoskeletal lineage. … This book is written for clinicians and basic science researchers interested in development of the musculoskeletal system and the promise of stem cells for treating patients with debilitating osteodegenerative diseases. … The authors provide excellent minireviews and great easy-to-follow research protocols. … The illustrations are educational and motivating. I recommend this book for research scientists interested in new approaches for treating patients with osteodegenerative diseases.” (Bruce A. Fenderson, Doody’s Review Service, June, 2011)
Editors and Affiliations
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Dept. of Cell Biology & Neuroscience, University of California at Riverside, Riverside, USA
Nicole I. Nieden