5.14 Conclusions
Both the microscopic and the gross anatomy of the fat mass should be appreciated by the liposuction surgeon. With this appreciation a better result can be expected, especially in understanding the caveat to not injure the dermis during a procedure.
The physiology depends on an appreciation for colloid osmotic pressure, which is significantly reduced by Klein’s tumescent solution. This wash-down of interstitial protein would enhance flow of the solution into the vascular space if it were not for the effect of epinephrine. Nevertheless using a massive amount of tumescent solution can overwhelm the right side of heart and cause congestive heart failure.
The fat mass is dynamic. The fact that adipocytes can differentiate and dedifferentiate is exciting in their application to redifferentiation into other cell types. Stem cells from lipoaspirate make more sense than bone marrow or embryonic sources.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
American Academy of Cosmetic Surgery. Liposuction Guidelines. 2003 http://www.cosmeticsurgery.org/Guidelines/ 2003_Liposuction_Guidelines.pdf.
Fawcett OW. A Textbook of Histology. New York: Chapman and Hall, 1994.
Kessel RG. Basic Medical Histology. New York: Oxford University Press, 1998.
Klein JA. Tumescent technique for regional anesthesia permits lidocaine doses of 35 mg/kg for liposuction: Peak plasma concentrations are diminished and delayed 12 hours. J Dermatol Surg Oncol. 1990;16:248–263.
Lalikos JF, Li YQ, Roth TP, et al. Biochemical assessment of cellular damage after adipocyte harvest. J Surg Res. 1997;70:95–100.
Weber KT, Swamynathan SK, Guntaka RV; et al. Angiotensis II and extracellular matrix homeostasis. Int J Biochem Cell Biol. 1999, 31:395–403.
Guyton AC. Capillary dynamics and exchange of fluid between the blood and the interstitial fluid. Text Book of Medical Physiology. 7th ed. Philadelphia: WB Saunders; 1986. p 348.
Kaminski MV, Haase T. Albumin and colloid pressure: Implications for fluid resuscitation. Crit Care Clin. 1992;9:311–322.
Pitman, GH. Liposuction & Aesthetic Surgery. Quality Medical Publishing, Inc., St. Louis, MO 1993.
Halvorsen YC, Wilkison WO, Briggs MR. Human adipocyte proteomics-a complementary way of looking at fat. Pharmacogenomics 2000; p 180.
Michaud EJ, Mynatt RL, Miltenberger RJ et al. Role of the agouti gene in obesity. J Endocrinol. 1997;155(2):207–209.
Zhang Y, Proenca R, Maffei M, Barone M, Leopold L, Friedman M. Positional cloning of the mouse obese gene and its human homologue. Nature. 1994;372:425–432.
Fried SK, Russell CD, Grauso NL, Brolin RE. Lipoprotein lipase regulation by insulin and glucocorticoid in subcutaneous and omental adipose tissue of obese women and men. J Clin Invest. 1993;92(5):2191–2198.
Kern PA, Svoboda ME, Eckel RH, Van Wyk JJ: Insulin-like growth factor action and production in adipocytes and endothelia1 cells from human adipose tissue. Diabetes 1989;38(6):710–717.
Boney CM, Moats-Staats BM, Stiles AD, D’Ercole AJ. Expression of insulin-like growth factor-I (IGF-1) and IGF-binding proteins during adipogenesis. Endocrinology1994; 135(5):1863–1868.
Cianflone K. Acylation stimulating protein and the adipocyte. J Endocrinol 1997;155(2):203–206.
Hotamisligil GS, Shargill NS, Spiegelman BM. Adipose expression of tumor necrosis factor-a1pha: direct role in obesity-linked resistance. Science 1993;259(5091):87–91.
Gottschling-Zeller H, Birgel M, Scriba D, Blum WF, Hauner H. Depot-specific release of leptin from subcutaneous and omental adipocytes in suspension culture effect of tumor necrosis factor-alpha and transforming growth factor-beta1. Eur J Endocrinol 1999;141(4):436–442.
Karlsson C, Lindell K, Ottosson M, Sjostrom L, Carlsson B, Carlsson LM: Human adipose tissue expresses angiotensinogen and enzymes required for its conversion to angiotensin II. J. Clin Endocrtnol Metab 1998;83(11):3925–3929.
Zechner R, Moser R, Newman TC, Fried SK, Breslow JL: Apolipoprotein E gene expression in mouse 3T3-L1 adipocytes and human adipose tissue and its regulation by differentiation and lipid content. J Biol Chem 1991;266(16):110583–10588.
Gautheier B, Robb M, McPherson R: Cholesteryl ester transfer protein gene expression during differentiation of human preadipocytes to adipocytes in primary culture. Atherosclerosis 1999;142(2):301–307.
Crandall DL, Quinet EM, Morgan GA, Busler DE, McHendry-Rinde B, Kral JG. Synthesis and secretion of plasminogen activator inhibitor-1 by human preadipocytes. J. Clin Endocrinol Metab 1999;84(9):3222–3227.
Samad F. Yamamoto K, Loskutoff DJ. Distribution and regulation of plasminogen activator inhibitor-l in murine adipose tissue in vivo. Induction by tumor necrosis factor-alpha and lipopolysaccharide. J Clin Invest 1996;97(1):37–46.
Ouchi N, Kihara S, Arita Y et al. Novel modulator for endothelial adhesion molecules: adipocyte-derived plasma protein adiponectin. Circulation 1999;100(25):2473–2476.
Cook KS, Min HY, Johnson D et al: Adipsin: a circulating serine protease homolog secreted by adipose tissue and sciatic nerve. Science 1987;237(4813):402–405.
Scherer PE, Williams S, Fogliano M, Baldini G, Lodish HF. A novel serum protein similar to C1q, produced exclusively in adipocytes. J Biol Chem 1995;270(45):26746–26749.
Peake PW, O’Grady S, Pussell BA, Charlesworth JA. Detection and quantification of the control proteins of the alternative pathway of complement 3T3-L1 adipocytes. Eur J Clin Invest 1997;27(11):922–927.
Claffey KP,. Wilkison WO, Spiefelman BM. Vascular endothelial growth factor. Regulation by cell differentiation and activated second messenger pathways. J Biol Chem 1992;267(23):16317–16322.
Fried SK, Bunkin DA, Greenburg AS. Omental and subcutaneous adipose tissues of obese subjects release interleukin-6: Depot difference and regulation by glucocorticoid. J. Clin Endocrinol. Metab 1998;83(3):847–850.
Smas CM, Sul HS. Pref-l, a protein containing EGF-like repeats, inhibits adipocyte differentiation. Cell 1993;73(4):725–734.
Levine JA, Jensen MD, Eberhardt NL, O’Brien T. Adipocyte macrophage colony-stimulating factor is a mediator of adipose tissue growth. J Clin Invest 1998;101(8):1557–1564.
Kaminski, M.V and Lopez de Vaughan R. The Key to Successful Longevity: Book One Nutrition. July 2004.
Sugihara H, Yonemitsu N, Miyabara S, Yum K. Primary cultures of unilocular fat cells: characteristics of growth factors. Int J Obes 1994;31: 42–9.
Cheng AYM, Deitel M, Roncar DAK. The biochemistry and molecular biology of human adipocyte reversion. Int J Obes 1994;18(Suppl. 2):112.
Van RLR, Roncari AK. Complete differentiation of adipocyte precursors. Cell Tissue Res 1978;195:317–39.
Van RLR, Bayliss CE, Roncari DAK. Cytological and enzymological characterization of adult human adipocyte precursors in culture. J Clin Invest 1976;58:699–704.
Hauner H. Prevention of adipose tissue growth. Int J Obes 1994;18(Suppl. 2):147.
Zuk PA, Ph.D., Zhu M, Mizuno H, Huang J B.S., Futrell W, Katz AJ, et al. Multilineage Cells from Human Adipose Tissue: Implications for Cell-Based Therapies. Tissue Eng 2001;7:211–24.
Lott KE, Awad HA, Gimble JM, Guilak F. Clonal Analysis of Multipotent Differentiation of Human Adipose-Derived Adult Stem Cells. Duke Med News 2004 Mar.8 News Release Available from: URL: http://www.dukemednews. org/news/article.php?id=7452.
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 2006 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Kaminski, M.V., Lopez de Vaughan, R.M. (2006). The Anatomy and Physiology Metabolism/Nutrition of Subcutaneous Fat. In: Liposuction. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-28043-X_5
Download citation
DOI: https://doi.org/10.1007/3-540-28043-X_5
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-28042-2
Online ISBN: 978-3-540-28043-9
eBook Packages: MedicineMedicine (R0)