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Laser Sources for Confocal Microscopy

  • Chapter
Handbook Of Biological Confocal Microscopy

Abstract

Laser assisted confocal microscopy has made a lot of progress over the past few years. Laser systems have become more modular and compact. There is an ever-increasing number of available laser excitation lines as well as an improvement in user friendliness and ease of use. At the same time, expansion of Web resources has provided easy access to a wealth of information. Our goal is both to aid the experienced and novice microscopist in quickly locating and sorting through the relevant laser information and to provide a means of avoiding common problems and pitfalls in the use of laser excitation in the various fluorescence techniques such as fluorescence correlation spectroscopy (FCS), fluorescence lifetime imaging microscopy (FLIM), fluorescence loss in photobleaching (FLIP), fluorescence recovery after photobleaching (FRAP), optical coherence tomography (OCT), second harmonic generation (SHG), single molecule detection (SMD), and single particle tracking (SPT). In this chapter we describe the characteristic properties of a number of lasers commonly used in fluorescence microscopy.

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Authors and Affiliations

  1. Department of Physics, Laboratory for Fluorescence Dynamics, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA

    Enrico Gratton

  2. Department of Cell Physiology, Microfluorimetry Section, Biomedical Institute, Hasselt University and Trans National University Limburg, and Institute for Materials Research IMO/IMOMEC, Diepenbeek, Belgium

    Martin J. vandeVen

Authors
  1. Enrico Gratton
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  2. Martin J. vandeVen
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Editors and Affiliations

  1. Department of Zoology, University of Wisconsin, Madison, WI, 53706, USA

    James B. Pawley

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Gratton, E., vandeVen, M.J. (2006). Laser Sources for Confocal Microscopy. In: Pawley, J. (eds) Handbook Of Biological Confocal Microscopy. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-45524-2_5

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