Abstract
Behavioral data were collected from several hundred mice and rats using a variety of experimental models of brain injury. The use of consistent protocols allowed compilation of these data, facilitating analyses of animal behaviors across experimental models, species, and gender. Spatial learning and sensorimotor/coordination data are presented, suggesting that, in general, rats performed better than mice both in the water maze and on the rotarod. Compared with females, males performed slightly better in the water maze and slightly worse on the rotarod. However, gender by species interactions accounted for both of these differences. Male rats performed better in the water maze than female rats, male mice, and female mice, which did not differ. Male mice performed worse on the rotarod than female mice, male rats, and female rats, which performed similarly. Furthermore, animals with subcortical injury were impaired in the water maze, but performed better than animals with cortical injuries. However, only animals with cortical injuries were impaired on the rotarod. Additional covariates, such as edema and lesion size, may further clarify these phenotypes. Overall, we provide evidence that abbreviated test batteries can be specifically designed to test deficits, depending on the species, gender, and model.
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References
Ajao DO, Pop V, Kamper JE et al (2012) Traumatic brain injury in young rats leads to progressive behavioral deficits coincident with altered tissue properties in adulthood. J Neurotrauma 29(11):2060–2074
Ashwal S, Ghosh N, Turenius CI, Dulcich M, Denham CM, Tone B, Hartman R, Snyder EY, Obenaus A (2014) Reparative effects of neural stem cells in neonatal rats with hypoxic ischemic injury are not influenced by host gender. Pediatr Res 75(5):603–611
Bertolizio G, Bissonnette B, Mason L, Ashwal S, Hartman R, Marcantonio S, Obenaus A (2011) Effects of hemodilution after traumatic brain injury in juvenile rats. Paediatr Anaesth. doi:10.1111/j.1460-9592.2011.03695.x
Chen W, Hartman RE, Ayer R, Marcantonio S, Kamper J, Tang J, Zhang JH (2009) Matrix metalloproteinases inhibition provides neuroprotection against hypoxia-ischemia in the developing brain. J Neurochem 111(3):726–736
Chen W, Ma Q, Suzuki H, Hartman RE, Tang J, Zhang JH (2011) Osteopontin reduced hypoxia-ischemia neonatal brain injury by suppression of apoptosis in a rat pup model. Stroke 42(3):764–769
Crawley J (2008) What’s wrong with my mouse – chapter 13. B chapter 1–20. Wiley-Liss, New York
Defelipe J (2011) The evolution of the brain, the human nature of cortical circuits, and intellectual creativity. Front Neuroanat 5:29
Donovan V, Bianchi A, Hartman R, Bhanu B, Carson MJ, Obenaus A (2012) Computational analysis reveals increased blood deposition following repeated mild traumatic brain injury. NeuroImage Clin 1(1):18–28
Fukuda AM, Adami A, Pop V, Bellone JA, Coats JS, Hartman RE, Ashwal S, Obenaus A, Badaut J (2013) Posttraumatic reduction of edema with aquaporin-4 RNA interference improves acute and chronic functional recovery. J Cereb Blood Flow Metab 33(0923559):1621–1632
Hartman RE, Lee JM, Zipfel GJ, Wozniak DF (2005) Characterizing learning deficits and hippocampal neuron loss following transient global cerebral ischemia in rats. Brain Res 1043(1–2):48–56
Hartman RE, Lekic T, Rojas H, Tang J, Zhang JH (2009) Assessing functional outcomes following intracerebral hemorrhage in rats. Brain Res 1280:148–157
Hartman RE, Rojas HA, Lekic T, Ayer R, Lee S, Jadhav V, Titova E, Tang J, Zhang JH (2008) Long-term effects of melatonin after intracerebral hemorrhage in rats. Acta Neurochir (Wien) 105:99–100
Hartman RE, Rojas H, Tang J, Zhang JH (2008) Long-term behavioral characterization of a rat model of intracerebral hemorrhage. Acta Neurochir (Wien) 105:125–126
Huang L, Coats JS, Mohd-Yusof A et al (2013) Tissue vulnerability is increased following repetitive mild traumatic brain injury in the rat. Brain Res 1499:109–120
Jonasson Z (2005) Meta-analysis of sex differences in rodent models of learning and memory: a review of behavioral and biological data. Neurosci Biobehav Rev 28:811–825
Kamper JE, Pop V, Fukuda AM, Ajao DO, Hartman RE, Badaut J (2013) Juvenile traumatic brain injury evolves into a chronic brain disorder: behavioral and histological changes over 6months. Exp Neurol 250:8–19
Lekic T, Hartman RE, Rojas H, Manaenko A, Chen W, Ayer R, Tang J, Zhang JH (2010) Protective effect of melatonin upon neuropathology, striatal function, and memory ability after intracerebral hemorrhage in rats. J Neurotrauma 27(3):627–637
Lekic T, Manaenko A, Rolland W, Krafft PR, Peters R, Hartman RE, Altay O, Tang J, Zhang JH (2012) Rodent neonatal germinal matrix hemorrhage mimics the human brain injury, neurological consequences, and post-hemorrhagic hydrocephalus. Exp Neurol 236(1):69–78
Lekic T, Manaenko A, Rolland W, Virbel K, Hartman RE, Tang J, Zhang J (2011) Neuroprotection by melatonin after germinal matrix hemorrhage in neonatal rats. Acta Neurochir (Wien) 111:201–206
Lindner MD, Schallert T (1988) Aging and atropine effects on spatial navigation in the Morris water task. Behav Neurosci 102:621–634
Manaenko A, Lekic T, Barnhart M, Hartman R, Zhang JH (2014) Inhibition of transforming growth factor-β attenuates brain injury and neurological deficits in a rat model of germinal matrix hemorrhage. Stroke 45:828–834
Obenaus A, Dilmac N, Tone B, Tian HR, Hartman R, Digicaylioglu M, Snyder EY, Ashwal S (2011) Long-term magnetic resonance imaging of stem cells in neonatal ischemic injury. Ann Neurol 69:282–291
Pop V, Sorensen DW, Kamper JE, Ajao DO, Murphy MP, Head E, Hartman RE, Badaut J (2013) Early brain injury alters the blood–brain barrier phenotype in parallel with β-amyloid and cognitive changes in adulthood. J Cereb Blood Flow Metab 33(2):205–214
Pop V, Sorensen DW, Kamper JE, Ajao DO, Paul Murphy M, Head E, Hartman RE, Badaut J, Murphy PM (2013) Early brain injury alters the blood – brain barrier phenotype in parallel with β-amyloid and cognitive changes in adulthood. J Cereb Blood Flow Metab 33(2):205–214
Recker R, Adami A, Tone B, Tian HR, Lalas S, Hartman RE, Obenaus A, Ashwal S (2009) Rodent neonatal bilateral carotid artery occlusion with hypoxia mimics human hypoxic-ischemic injury. J Cereb Blood Flow Metab 29:1305–1316
Rolland WB, Lekic T, Krafft PR, Hasegawa Y, Altay O, Hartman R, Ostrowski R, Manaenko A, Tang J, Zhang JH (2013) Fingolimod reduces cerebral lymphocyte infiltration in experimental models of rodent intracerebral hemorrhage. Exp Neurol 241:45–55
Royle SJ, Collins FC, Rupniak HT, Barnes JC, Anderson R (1999) Behavioural analysis and susceptibility to CNS injury of four inbred strains of mice. Brain Res 816:337–349
Snyder JS, Choe JS, Clifford MA, Jeurling SI, Hurley P, Brown A, Kamhi JF, Cameron HA (2009) Adult-born hippocampal neurons are more numerous, faster maturing, and more involved in behavior in rats than in mice. J Neurosci 29:14484–14495
Voyer D, Voyer S, Bryden MP (1995) Magnitude of sex differences in spatial abilities: a meta-analysis and consideration of critical variables. Psychol Bull 117:250–270
Whishaw IQ (1996) An endpoint, descriptive, and kinematic comparison of skilled reaching in mice (Mus musculus) with rats (Rattus norvegicus). Behav Brain Res 78:101–111
Whishaw IQ, Metz GAS, Kolb B, Pellis SM (2001) Accelerated nervous system development contributes to behavioral efficiency in the laboratory mouse: a behavioral review and theoretical proposal. Dev Psychobiol 39:151–170
Whishaw IQ, Tomie JA (1997) Of mice and mazes: similarities between mice and rats on dry land but not water mazes. Physiol Behav 60:1191–1197
Acknowledgments
We would like to thank our collaborators at Loma Linda University, including the laboratories of Drs. John Zhang, Andre Obenaus, Jiping Tang, Stephen Ashwal, and Jerome Badaut.
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The authors declare that they have no conflict of interest.
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Hartman, R.E., Thorndyke, E.C. (2016). Patterns of Behavioral Deficits in Rodents Following Brain Injury Across Species, Gender, and Experimental Model. In: Applegate, R., Chen, G., Feng, H., Zhang, J. (eds) Brain Edema XVI. Acta Neurochirurgica Supplement, vol 121. Springer, Cham. https://doi.org/10.1007/978-3-319-18497-5_12
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DOI: https://doi.org/10.1007/978-3-319-18497-5_12
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