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Aptamer-Based Enzyme Capture Assay for Measurement of Plasma Thrombin Levels

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Nucleic Acid Aptamers

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1380))

Abstract

The quantification of circulating thrombin is a valuable tool to accurately assess the activity of the blood coagulation system. Here, we describe the combined application of the thrombin-specific reversible active-site inhibitor argatroban and the DNA-aptamer HD1-22 for conduction of an enzyme capture assay for reliable measurement of plasma thrombin levels.

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Author information

Authors and Affiliations

  1. Institute for Experimental Haematology and Transfusion Medicine, University of Bonn Medical Centre, Bonn, Germany

    Jens Müller, Tobias Becher & Bernd Pötzsch

  2. Life & Medical Sciences Institute (LIMES), University of Bonn, Bonn, Germany

    Günter Mayer

Authors
  1. Jens Müller
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  2. Tobias Becher
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  3. Günter Mayer
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  4. Bernd Pötzsch
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Corresponding author

Correspondence to Jens Müller .

Editor information

Editors and Affiliations

  1. University of Bonn, Bonn, Germany

    Günter Mayer

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© 2016 Springer Science+Business Media New York

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Müller, J., Becher, T., Mayer, G., Pötzsch, B. (2016). Aptamer-Based Enzyme Capture Assay for Measurement of Plasma Thrombin Levels. In: Mayer, G. (eds) Nucleic Acid Aptamers. Methods in Molecular Biology, vol 1380. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3197-2_15

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  • DOI: https://doi.org/10.1007/978-1-4939-3197-2_15

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3196-5

  • Online ISBN: 978-1-4939-3197-2

  • eBook Packages: Springer Protocols

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