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Guidelines to Compose an Ideal Bacteriophage Cocktail

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Bacteriophage Therapy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1693))

Abstract

Correctly designed bacteriophage therapeutics are the cornerstone for a successful outcome of bacteriophage therapy. Here we overview strategies on how to choose bacteriophages and their bacterial hosts at different steps of a bacteriophage cocktail development in order to comply with all quality and safety requirements based on the already existing essentially empirical experience in bacteriophage therapy and current accomplishments in modern biomedical sciences. A modification of the classic Appelmans’ method (1922) to assess stability of bacteriophage activity in liquid media is presented in order to improve the overall performance of therapeutic bacteriophages individually and collectively in the cocktail.

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Author information

Authors and Affiliations

  1. Laboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, Bruynstraat 1, Brussels, 1120, Belgium

    Maia Merabishvili, Jean-Paul Pirnay & Daniel De Vos

Authors
  1. Maia Merabishvili
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  2. Jean-Paul Pirnay
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  3. Daniel De Vos
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Corresponding author

Correspondence to Maia Merabishvili .

Editor information

Editors and Affiliations

  1. LIBRO-Laboratory of Research in Biofilms Rosário Oliveira, CEB – Centre of Biological Engineering, University of Minho, Braga, Portugal

    Joana Azeredo

  2. CEB – Centre of Biological Engineering, University of Minho, Braga, Portugal

    Sanna Sillankorva

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Merabishvili, M., Pirnay, JP., De Vos, D. (2018). Guidelines to Compose an Ideal Bacteriophage Cocktail. In: Azeredo, J., Sillankorva, S. (eds) Bacteriophage Therapy. Methods in Molecular Biology, vol 1693. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7395-8_9

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  • DOI: https://doi.org/10.1007/978-1-4939-7395-8_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7394-1

  • Online ISBN: 978-1-4939-7395-8

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