Abstract
The assessment of developmental toxicology data is a critical aspect of hazard evaluation for pharmaceuticals and environmental chemicals. Skeletal examination is an essential part of prenatal developmental toxicity studies of chemicals as well as pesticides and comprises evaluation of both cartilaginous and ossified skeletal components. Various techniques are published in the literature to process and double-stain skeletons of common laboratory animals which are all based on staining of the cartilage with Alcian Blue and staining of ossified bones with Alizarin Red S along with maceration (clearing) of the surrounding soft tissue. The staining of the cartilage allows the examination to assess ossified structures and their underlying cartilage in a single step.
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References
Kimmel CA, Trammell C (1981) A rapid procedure for routine double staining of cartilage and bone in fetal and adult animals. Stain Technol 56:271–273
Beltrame D, Giavini E (1990) Morphological abnormalities in experimental teratology: need for a standardization of current terminology. Cong Anom 30(3):187–195
Chahoud I, Buschmann J, Clark R et al (1999) Classification terms in developmental toxicology: need for harmonisation. Reprod Toxicol 13:77–82
Solecki R, Bergmann B, Bürgin H et al (2003) Harmonization of rat fetal external and visceral terminology and classification. Report of the fourth workshop on the terminology in developmental toxicology. Reprod Toxicol 17:625–637
Wise D, Beck S, Beltrame D et al (1997) Terminology of developmental abnormalities in common laboratory mammals (version 1). Teratology 55:249–292
Makris SL, Solomon HM, Clark R et al (2009) Terminology of developmental abnormalities in common laboratory mammals (version 2). Birth Defects Res B Dev Reprod Toxicol 86:227–327
Acknowledgments
Thanks to E. Hoffmann, E. Lisador, and I. Kemmer for expert technical assistance.
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Schneider, S. (2013). Skeletal Examination by Double Staining for Ossified Bone and Cartilaginous Tissue. In: Barrow, P. (eds) Teratogenicity Testing. Methods in Molecular Biology, vol 947. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-131-8_17
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DOI: https://doi.org/10.1007/978-1-62703-131-8_17
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-130-1
Online ISBN: 978-1-62703-131-8
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