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Tumor Targeting by a Carbohydrate Ligand-Mimicking Peptide

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Glycosyltransferases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1022))

Abstract

Annexin A1 (Anxa1) is a highly specific surface marker of tumor vasculature. We used peptide-displaying phage technology to identify a carbohydrate ligand-mimicking 7-mer peptide, IFLLWQR (IF7), which can target Anxa1 in tumor vasculature. Here, we describe the binding activity of carbohydrate to Anxa1, Anxa1 to heparan sulfates, and the therapeutic potential of IF7 conjugated with anticancer drugs in tumor targeting.

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Acknowledgments

This study was supported by a grant-in-aid for Scientific Research 23791737 from the Japan Society for the Promotion of Science (SH) and NIH grant P01CA071932 (MNF), DoD Breast Cancer Research IDEA grant DAMD17-02-1-0311 (MNF), and a Susan Komen Breast Cancer Research grant BCTR0504175 (MNF).

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Authors and Affiliations

  1. Department of Urology, Hirosaki University School of Medicine, Hirosaki, Aomori, Japan

    Shingo Hatakeyama, Yuki Tobisawa & Chikara Ohyama

  2. Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka, Japan

    Toshiaki K. Shibata & Kazuhiro Sugihara

  3. Tumor Microenvironment Program, Cancer Center, Sanford-Burnham Medical Research Institute, La Jolla, CA, USA

    Michiko N. Fukuda

Authors
  1. Shingo Hatakeyama
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  2. Toshiaki K. Shibata
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  3. Yuki Tobisawa
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  4. Chikara Ohyama
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  5. Kazuhiro Sugihara
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  6. Michiko N. Fukuda
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Editor information

Editors and Affiliations

  1. Department of Medicine, Queen's University, Kingston, Ontario, Canada

    Inka Brockhausen

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Hatakeyama, S., Shibata, T.K., Tobisawa, Y., Ohyama, C., Sugihara, K., Fukuda, M.N. (2013). Tumor Targeting by a Carbohydrate Ligand-Mimicking Peptide. In: Brockhausen, I. (eds) Glycosyltransferases. Methods in Molecular Biology, vol 1022. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-465-4_28

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  • DOI: https://doi.org/10.1007/978-1-62703-465-4_28

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-464-7

  • Online ISBN: 978-1-62703-465-4

  • eBook Packages: Springer Protocols

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