Abstract
Microglia are the inflammatory cells of the brain and are activated in neuropathological conditions. To study the biology of microglia, these cells can be isolated from the brain and analyzed in terms of pro- and anti-inflammatory cytokine production, involvement of intracellular signaling pathways upon inflammatory stimuli, phagocytosis, and several more biological aspects to understand their role in the brain. In this book chapter, I will discuss microglial cells and describe how these cells can be cultured from a postnatal mouse and rat brain.
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References
Ransohoff RM, Brown MA (2012) Innate immunity in the central nervous system. J Clin Invest 122(4):1164–1171
Lambertsen KL et al (2011) Differences in origin of reactive microglia in bone marrow chimeric mouse and rat after transient global ischemia. J Neuropathol Exp Neurol 70(6):481–494
Giulian D, Baker TJ (1985) Peptides released by ameboid microglia regulate astroglial proliferation. J Cell Biol 101(6):2411–2415
Kalla R et al (2003) Loss of microglial ramification in microglia-astrocyte cocultures: involvement of adenylate cyclase, calcium, phosphatase, and Gi-protein systems. Glia 41(1):50–63
Tanaka J et al (1999) Morphological differentiation of microglial cells in culture: involvement of insoluble factors derived from astrocytes. Neurosci Res 34(4):207–215
Lambertsen KL et al (2009) Microglia protect neurons against ischemia by synthesis of tumor necrosis factor. J Neurosci 29(5):1319–1330
Giulian D et al (1995) Cell surface morphology identifies microglia as a distinct class of mononuclear phagocyte. J Neurosci 15(11):7712–7726
Tanaka J, Maeda N (1996) Microglial ramification requires nondiffusible factors derived from astrocytes. Exp Neurol 137(2):367–375
Giulian D, Ingeman JE (1988) Colony-stimulating factors as promoters of ameboid microglia. J Neurosci 8(12):4707–4717
Gehrmann J (1996) Microglia: a sensor to threats in the nervous system? Res Virol 147(2–3):79–88
Fujita H et al (1996) Effects of GM-CSF and ordinary supplements on the ramification of microglia in culture: a morphometrical study. Glia 18(4):269–281
Aloisi F et al (2000) Functional maturation of adult mouse resting microglia into an APC is promoted by granulocyte-macrophage colony-stimulating factor and interaction with Th1 cells. J Immunol 164(4):1705–1712
Lee SC et al (1993) Macrophage colony-stimulating factor in human fetal astrocytes and microglia. Differential regulation by cytokines and lipopolysaccharide, and modulation of class II MHC on microglia. J Immunol 150(2):594–604
Ponomarev ED et al (2005) Development of a culture system that supports adult microglial cell proliferation and maintenance in the resting state. J Immunol Methods 300(1–2):32–46
Flanary BE, Streit WJ (2004) Progressive telomere shortening occurs in cultured rat microglia, but not astrocytes. Glia 45(1):75–88
Floden AM, Combs CK (2007) Microglia repetitively isolated from in vitro mixed glial cultures retain their initial phenotype. J Neurosci Methods 164(2):218–224
Acknowledgement
This work has been supported by grants provided by the Swedish Research Council grant no. 2012–2229 by the Gyllenstierna Krapperup, the Royal Physiographic Society, Swedish stroke association, Crafoord, A.E. Berger, Wiberg, Bergvall, G & J Kock foundations.
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Deierborg, T. (2013). Preparation of Primary Microglia Cultures from Postnatal Mouse and Rat Brains. In: Joseph, B., Venero, J. (eds) Microglia. Methods in Molecular Biology, vol 1041. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-520-0_4
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DOI: https://doi.org/10.1007/978-1-62703-520-0_4
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-519-4
Online ISBN: 978-1-62703-520-0
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