Abstract
The prognosis of renal cell carcinoma (RCC) varies dependent on histologic tumor subtypes. However, differentiation between RCC types may sometimes be difficult on histologic grounds alone. Furthermore, the prognostic value of histologic parameters for the individual prognosis is limited. Additional information on the molecular level seems necessary to obtain more certainty in diagnostic and prognostic evaluation. By investigating genetic alterations in different RCC subtypes, we sought to obtain a genotype-phenotype correlation. Eighty-two clear-cell, 53 papillary, 23 chromophobe RCCs and 26 renal oncocytomas were investigated. Comparative genomic hybridization (CGH) was performed on DNA from paraffin-embedded tissue samples. DNA was isolated from tumor areas by microdissection and amplified by degenerated oligonucleotide primed polymerase chain reaction (DOP-PCR). CGH was performed according to standard protocols. We were able to detect specific alterations in each RCC subtype: clear cell RCC showed -3p, +5/5q, -8p, -9, -14, -18; papillary (chromophilic) RCC gains of chromosomes 7, 17, 16, 3, 12; chromophobe RCC loss of chromosomes 1, 2, 6, 10, 13, 17, 21; renal oncocytomas loss of chromosomes 1/1p and 14. Furthermore, for clear cell RCC, it was possible to define alterations which are associated with metastatic disease: loss of 9, 10, 14. Our results demonstrate that each RCC subtype is characterized by distinct genetic alterations. The definition of genetic alterations seems helpful for a tumor typing especially when morphology is equivocal. Therefore, genetic analyses represent a powerful diagnostic and prognostic tool for RCC
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Reference
Guinan P, Sobin LH, Algaba F, Badellino F, Karneyama S, MacLennan G, Novick A (1997) TNM staging of renal cell carcinoma: Workgroup No. 3. Union International Contre le Cancer (UICC) and the American Joint Committee on Cancer (AJCC) Cancer 80:992–933
Storkel, S (1999) [Epithelial tumors of the kidney. Pathological subtyping and cytogenetic correlation]. Urologe A 38:425–432
Chudoba IHT, Senger G, Claussen U, Haas OA (1997) Comparative genomic hybridization using DOP-PCR amplified DNA from a small number of nuclei. Cs Pediat 52:519–521
Kovacs G (1994) The value of molecular genetic analysis in the diagnosis and prognosis of renal cell tumours. World J Urol 12:64–68
van den Berg E, van der Hout AH, Oosterhuis JW, Storkel S, Dijkhuizen T, Dam A, Zweers HM, Mensink HJ, Buys CH, de Jong B (1993) Cytogenetic analysis of epithelial renal-cell tumors: relationship with a new histopathological classification. Int J Cancer 55:223–227
Velickovic M, Delahunt B, Grebe SK (1999) Loss of heterozygosity at 3pl4.2 in clear cell renal cell carcinoma is an early event and is highly localized to the FHIT gene locus. Cancer Res 59:1323–1326
Bernues M, CasadevallC, Miro R, Caballin MR, Gelabert A, Ejarque MJ, Chechile G, Egozcue J (1998) Analysis of 3p allelic losses in renal cell carcinomas: comparison with cytogenetic results. Cancer Genet Cytogenet 107:121–124
Kattar MM, Grignon DJ, Wallis T, Haas GP, Sakr WA, Pontes JE, Visscher DW (1997) Clinicopathologic and interphase cytogenetic analysis of papillary (chromophilic) renal cell carcinoma. Mod Pathol 10:1143–1150
Kovacs G, Fuzesi L, Emanual A, Kung HF (1991) Cytogenetics of papillary renal cell tumors. Genes Chromosomes Cancer 3:249–255
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2003 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Junker, K., Weirich, G., Amin, M.B., Moravek, P., Hindermann, W., Schubert, J. (2003). Genetic Subtyping of Renal Cell Carcinoma by Comparative Genomic Hybridization. In: Allgayer, H., Heiss, M.M., Schildberg, F.W. (eds) Molecular Staging of Cancer. Recent Results in Cancer Research, vol 162. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59349-9_15
Download citation
DOI: https://doi.org/10.1007/978-3-642-59349-9_15
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-63945-6
Online ISBN: 978-3-642-59349-9
eBook Packages: Springer Book Archive