Abstract
The blood-brain barrier (BBB) was studied in rats with electrolyte-induced demyelination (EID), an experimental model for central pontine myelinolysis. Intravenously injected peroxidase was extravasated at 3 h post hypertonic saline injection (PHS) into regions frequently involved in EID. Increased pinocytotic activity and focal interendothelial gaps were seen at 3 h PHS and less frequently at 48 h PHS. Measurement of total cerebral water content revealed an increase during the hyponatremic phase. This was followed by a marked increase at 3 h PHS with continued increment at 48 h PHS. Intracellular edema with accumulation of fluid within neurites and astrocytic processes was noted during the hyponatremic phase, whereas extracellular edema developed after hypertonic saline injection. The implications of disrupted BBB and its role in the pathogenesis of EID are discussed.
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References
Adams JH (1961) Central pontine myelinolysis. In: Jacob H (ed) Proceedings of the Fourth International Congress of Neuropathology, Munich, W. Germany, vol 3. Thieme, Stuttgart, pp 303–308
Adams RD, Victor M, Mancall EL (1959) Central pontine myelinolysis: a hitherto unpublished disease occurring in alcoholic and malnourished patients. Arch Neurol Psychiatry 81: 154–172
Aleu F, Terry R (1963) Central pontine myelinolysis: a report of two cases. Arch Pathol 76: 140–146
Bass NH (1968) Pathogenesis of myelin lesions in experimental cyanide encephalopathy. Neurology 18: 167–177
Beggs JL, Waggener JD (1976) Transendothelial vesicular transport of protein following compression injury to the spinal cord. Lab Invest 34: 428–439
Berry K, Olszewski J (1963) Central pontine myelinolysis. Neurology 13: 531–537
Bodenheimer TS, Brightman MW (1968) A blood-brain barrier to peroxidase in capillaries surrounded by perivascular spaces. Am J Anat 122: 249–239
Brightman MW, Klatzo I, Olsson Y, Reese TS (1970) The blood-brain barrier to proteins under normal and pathological conditions. J Neurol Sci 10: 215–239
Chason JL, Landers JW, Gonzales JE (1964) Central pontine myelinolysis. J Neurol Neurosurg Psychiatry 27: 317–325
Dodge PR, Crawford JD, Probst JH (1960) Studies in experimental water intoxication. Arch Neurol 3: 513–529
Dorovini-Zis K, Bowman PD, Betz AL, Goldstein GW (1987) Hyperosmotic urea reversibly opens the tight junctions between brain capillary endothelial cells in cell culture. J Neuropathol Exp Neurol 46: 130–140
Feder N, Reese TS, Brightman MW (1969) Microperoxidase, a new tracer of low molecular weight. A study of the interstitial compartments of the mouse brain (abstract). J Cell Biol 43: 35a-36a
Finlayson MH, Snider S, Oliva LA, Gault MH (1973) Cerebral and pontine myelinolysis: two cases with fluid and electrolyte imbalance and hypotension. J Neurol Sci 18: 399–409
Fishman RA (1974) Cell volume, pumps, and neurological function: brain's adaptation to osmotic stress. Res Publ Assoc Res Nerv Ment Dis 53: 159–171
Hansson HA, Johansson BB (1980) Induction of pinocytosis in cerebral vessels by acute hypertension and by hyperosmolar solutions. J Neurosci Res 5: 183–190
Holliday MA, Kalayci MN, Harrah J (1968) Factors that limit brain volume changes in response to acute and sustained hyper- und hyponatremia. J Clin Invest 47: 1916–1928
Klavins JV (1963) Central pontine myelinolysis. J Neuropathol Exp Neurol 22: 307–317
Kleinschmidt-DeMasters BK, Norenberg MD (1982) Neuropathologic observations in electrolyte-induced myelinolysis in the rat. J Neuropathol Exp Neurol 41: 67–80
Messert B, Orrison WW, Hawkins MJ, Quagleri CE (1979) Central pontine myelinolysis: considerations on etiology, diagnosis and treatment. Neurology 29: 147–160
Petito CK, Pulsinelli WA, Jacobson G, Plum F (1982) Edema and vascular permeability in cerebral ischemia: comparison between ischemic and neuronal damage and infarction. J Neuropathol Exp Neurol 41: 423–436
Powers JM, McKeever PE (1976) Central pontine myelinolysis: an ultrastructural and elemental study. J Neurol Sci 29: 65–81
Rapoport SI, Hori M, Klatzo I (1971) Testing of a hypothesis for osmotic opening of the blood-brain barrier. Am J Physiol 223: 323–331
Reese TS, Karnovsky MJ (1976) Fine structural localization of the blood-brain barrier to exogenous peroxidase. J Cell Biol 34: 207–217
Rojiani AM, Prineas JW, Cho E-S (1987) Protective effect of steroids in electrolyte induced demyelination. J Neuropathol Exp Neurol 46: 495–505
Sima A, Bradvik B (1976) Central pontine myelinolysis. A case report. Acta Pathol Microbiol Scand [A] 84: 73–78
Sotos JF, Dodge PR, Meera P, Talbot NB (1960) Studies in experimental hypertonicity: pathogenesis of the clinical syndrome, biochemical abnormalities and cause of death. Pediatrics 26: 925–938
Tomlinson BE, Pierides AM, Bradley WG (1976) Central pontine myelinolysis: two cases with associated electrolyte disturbance. Q J Med 45: 373–386
Westergaard E (1975) Enhanced vesicular transport of exogenous peroxidase across cerebral vessels induced by serotonin. Acta Neuropathol (Berl) 32: 27–42
Westergaard E (1977) The blood-brain barrier to horseradish peroxidase under normal and experimental conditions. Acta Neuropathol (Berl) 39: 181–187
Wright DG, Laureno R, Victor M (1979) Pontine and extrapontine myelinolysis. Brain 102: 361–385
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Rojiani, A.M., Prineas, J.W. & Cho, ES. Electrolyte-induced demyelination in rats. Acta Neuropathol 88, 287–292 (1994). https://doi.org/10.1007/BF00310371
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DOI: https://doi.org/10.1007/BF00310371