Abstract
We studied the healing of tibial double osteotomies in rats during clodronate administration 10 mg/kg daily subcutaneously for 6 weeks. Clodronate treatment did not inhibit the endochondral bone formation in callus or in the epiphyseal plate. The differentiation of osteoblasts into mature osteocytes was disturbed, and the bone structure around the osteocytes was not normal. In the clodronatetreated group, the amount of newly formed bone matrix seemed increased, but mineralization of the bone matrix was delayed. The bundle bone trabeculae in callus were not so spatially well organized as in the controls. No delay in the union of the bone segment by new bone was seen during clodronate administration.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bauer HCF, Törngvist H, Nilsson OS (1986) Effects of biphosphonates on the incorporation of calcium-45 and 3H-proline in orthopic and in demineralized matrix-induced bone in rats. Bone 7:129
Chappard D, Petitjean M, Alexandre C, Vico L, Minaire P, Riffat G (1991) Cortical osteoclasts are less sensitive to etidronate than trabecular osteoclasts. J Bone Joint Res 6:673
Flanagan AM, Chambers TJ (1989) Dichloromethylenebiphosphonate (Cl2MBP) inhibits bone resorption through injury to osteoclasts that resorb Cl2MBP-coated bone. Bone Miner 6:33
Fornasserier VL (1977) Osteoid: an ultrastructural study. Hum Pathol 8:243
Giannini S, D'Angelo A, Malvasi L, Castrignano R, Tronca R, Liberto L, Nobile M, Crepaldi G (1993) Effects of one-year cyclical treatment with clodronate on posttraumatic bone loss. Bone 14:137
Jung A, Bornand J, Mermillod B, Edouard C, Meunier PJ (1984) Inhibition by diphosphonates of bone resorption induced by the Walker tumor of the rat. Cancer Res 44:3307
Kanis JA, McCloskey EV, Sirtori P, Khan S, Fern D, Eyres K, Aaron J, Beneton MN (1993) Rationale for the use of clodronate in osteoporosis. Osteopor Int 3 (Suppl 2):23–28
Lepore L, Pennesi M, Barbi E, Pozzi R (1991) Treatment and prevention of osteoporosis in juvenile chronic arthritis with disodium clodronate. Clin Exp Rheumatol 9 (Suppl 6):33
Miller SC, Jee WSS (1979) The effect of dichloromethylene diphosphonate, a pyrophosphate analog, on bone and bone cell structure in the growing rat. Anat Rec 193:439
Nyman MT, Paavolainen P, Lindholm TS (1993) Clodronate increases the calcium content in fracture callus: an experimental study in rats. Arch Orthop Trauma Surg 112:228
Nyman MT, Paavolainen P, Holmströnm T, Penttinen R, Verkasalo M (1993) Clodronate reduces plate osteopenia in the rabbit. Acta Orthop Scand 64:50
Raina V (1972) Normal osteoid tissue. J Clin Pathol 25:229
Schenk R, Merz WA, Muhlbauer R, Russell RGG, Fleisch H (1973) Effect of ethane- 1-hydroxy-1,1-diphosphonate (EHDP) and dichloromethylene diphosphonate (CI2MDP) on the calcification and resorption of cartilage and bone in the tibial epiphysis and metaphysis of rats. Calcif Tissue Res 11:190
Vaes G (1988) Cellular biology and biomechanical mechanism of bone resorption. A review of recent developments on the formation, activation and mode of action of osteoclasts. Clin Orthop 231:239
Vanderwiel CJ (1980) An ultrastructural study of the components which make up the resting surface of bone. Metab Bone Dis 2 (Suppl):109
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nyman, M.T., Gao, T., Lindholm, T.C. et al. Healing of a tibial double osteotomy is modified by clodronate administration. Arch Orthop Trauma Surg 115, 111–114 (1996). https://doi.org/10.1007/BF00573453
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00573453