Summary
The addition of lactalblumin hydrolysate and yeast extract to Eagle's medium enhanced the growth ofTheileria annulata infected lymphocytes and made establishment of cultures with comparatively small numbers of infected cells possible. No adverse effect on growth was shown when ovine or equine serum was substituted for bovine serum in the medium.
Résumé
L'addition d'hydrolysat de lactalblumine et d'extrait de levure (L au milieu de Eagle augmente la croissance deTheileria annulata infectant le lymphocytes et permet la réalisation de cultures à partir d'un faible nombre de cellules infectées. Aucun effet contraire sur le développement ne s'est manifesté lorsque le sérum de mouton ou de cheval dans le milieu de culture, était remplacé par du sérum bovin.
Resumen
La adición de lactalbúmina hidrolizada y extracto de levadura al medio de Eagle, aumentó de linfocitos infectados conTheileria annulata, haciendo posible el establecimento de cultivos con un número pequeño de células infectadas. No se observó efecto adverso en el crecimiento celular, cuando el suero ovino o equino en el medio fue sustituído por suero bovino.
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Hooshmand-Rad, P. The growth ofTheileria annulata infected cells in suspension culture. Trop Anim Health Prod 7, 23–28 (1975). https://doi.org/10.1007/BF02383238
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DOI: https://doi.org/10.1007/BF02383238