Skip to main content

Advertisement

SpringerLink
Log in

Control of plasma 1,25-(OH)2-vitamin D concentrations by calcium and phosphorus in the rat: Effects of hypophysectomy

  • Published:
Calcified Tissue International Aims and scope Submit manuscript

Summary

The mechanism by which dietary phosphate deprivation elevates plasma 1,25-(OH)2-D levels is not known. To evaluate the role of the pituitary in regulating plasma 1,25-(OH)2-D concentrations, the responses of plasma 1,25-(OH)2-D to dietary phosphate deprivation and, separately, to dietary calcium deprivation were evaluated in intact and hypophysectomized male rats. Among intact and hypophysectomized rats eating normal diets, plasma 1,25-(OH)2-D levels averaged 228±76 and 148±62 pmol/1, respectively (P<0.01). During dietary phosphate deprivation, plasma 1,25-(OH)2-D levels rose to 1160±260 in intact rats and fell to 90±26 pmol/l in hypophysectomized rats (P<0.001). By contrast, during dietary calcium deprivation, plasma 1,25-(OH)2-D levels rose in both intact and hypophysectomized animals to 856±107 and 742±279 pmol/l, respectively (NS). In response to dietary phosphate deprivation, serum calcium concentrations rose as 1,25-(OH)2-D concentrations rose in intact rats but remained at control levels in hypophysectomized rats. These results support the hypothesis that a pituitary hormone acting either directly or indirectly on the kidney mediates the increase in plasma 1,25-(OH)2-D during dietary phosphate deprivation. The hypercalcemia that occurs in rats during dietary phosphate deprivation appears to depend on the elevation of plasma 1,25-(OH)2-D.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Hughes, M. R., Haussler, M. R., Wergedal, J., Baylink, D. J.: Regulation of serum 1α,25-dihydroxyvitamin D3 by calcium and phosphate in the rat, Science190:578, 1975

    CAS  PubMed  Google Scholar 

  2. Rader, J. I., Baylink, D. J., Hughes, M. R., Safilian, E. F., Haussler, M. R.: Calcium and phosphorus deficiency in rats: effects on PTH and 1,25-dihydroxyvitamin D3, Am. J. Physiol.236:E118–122, 1979

    CAS  PubMed  Google Scholar 

  3. Tanaka, Y., DeLuca, H. F.: The control of 25-hydroxyvitamin D metabolism by inorganic phosphorus, Arch. Biochem. Biophys.154:566, 1973

    Article  CAS  PubMed  Google Scholar 

  4. Gray, R. W.: Effects of age and sex on the regulation of plasma 1,25-(OH)2-D by phosphorus in the rat, Calcif. Tissue Int.33, 477–484, 1981

    CAS  PubMed  Google Scholar 

  5. Spanos, E., Burnett, D., MacIntyre, I., Pike, J. W., Safilian, E. J., Haussler, M. R.: Effect of growth hormone on vitamin D metabolism, Nature273:246–247, 1978

    Article  CAS  PubMed  Google Scholar 

  6. Trechsel, U., Bonjour, J.-P., Fleisch, H.: Regulation of the metabolism of 25-hydroxyvitamin D3 in primary cultures of chick kidney cells, J. Clin. Invest.64:206–217, 1979

    Article  CAS  PubMed  Google Scholar 

  7. Caldas, A. E., Gray, R. W., Lemann, J. Jr.: The simultaneous measurement of vitamin D metabolites in plasma: studies in healthy adults and in patients with calcium nephrolithiasis, J. Lab. Clin. Med.91:840–849, 1978

    CAS  PubMed  Google Scholar 

  8. Gray, R. W., Wilz, D. R., Caldas, A. E., Lemann, J. Jr.: The importance of phosphate in regulating plasma 1,25-(OH)2-vitamin D levels in humans: studies in healthy subjects, in calcium-stone formers and in patients with primary hyperparathyroidism, J. Clin. Endocrinol. Metab.45:299–306, 1977

    CAS  PubMed  Google Scholar 

  9. Snedecor, G. W., Cochran, W. G.: Statistical Methods, ed. 6. Iowa State University Press, Ames, Iowa, 1967

    Google Scholar 

  10. Garabedian, M., Holick, M. F., DeLuca, H. F., Boyle, I. T.: Control of 25-hydroxycholecalciferol metabolism by the parathyroid glands, Proc. Natl. Acad. Sci. U.S.A.69:1673–1976, 1972

    CAS  PubMed  Google Scholar 

  11. Bilezikian, J. P., Canfield, R. E., Jacobs, T. P., Polay, J. S., D'Adama, A. P., Eisman, J. A., DeLuca, H. F.: Response of 1α,25-dihydroxyvitamin D3 to hypocalcemia in human subjects, N. Engl. J. Med.299:437, 1978

    Article  CAS  PubMed  Google Scholar 

  12. Adams, N. D., Gray, R. W., Lemann, J. Jr.: The effects of oral CaCO3 loading and dietary calcium deprivation on plasma 1,25-dihydroxyvitamin D concentrations in healthy adults, J. Clin. Endocrinol. Metab.48:1008–1016, 1979

    Article  CAS  PubMed  Google Scholar 

  13. Gertner, J. M., Horst, R. L., Broadus, A. E., Rasmussen, H., Gene, M.: Parathyroid function and vitamin D metabolism during human growth hormone replacement, J. Clin. Endocrinol. Metab.49:185–188, 1979

    CAS  PubMed  Google Scholar 

  14. Eskildsen, P. C., Lund, B. J., Sorensen, O. H., Lund, B. I., Bishop, J. E., Norman, A. W.: Acromegaly and vitamin D metabolism: effect of bromocriptine treatment, J. Clin. Endocrinol. Metab.49:484–486, 1979

    CAS  PubMed  Google Scholar 

  15. Brown, D. J., Spanos, E., Raptis, P., MacIntyre, I.: Effect of pregnancy, acromegaly, primary hyperparathyroidism and prolactinoma on 1,25-dihydroxyvitamin D in man. In A. W. Norman, K. Schaefer, D. v. Herrath, H.-G. Grigoleit, J. W. Coburn, H. F. DeLuca, E. B. Mawer, T. Suda (eds.): Vitamin D Basic Research and Its Clinical Application, pp. 625–635. Walter de Gruyter, New York, 1979

    Google Scholar 

  16. Kumar, R., Merimee, T. J., Silva, P., Epstein, F. H.: The effect of chronic excess or deficiency of growth hormone on plasma 1,25-dihydroxyvitamin D levels in man. In A. W. Norman, K. Schaefer, D. v. Herrath, H.-G. Grigoleit, J. W. Coburn, H. F. DeLuca, E. B. Mawer, T. Suda (eds.): Vitamin D Basic Research and Its Clinical Application, pp. 1005–1009. Walter de Gruyter, New York, 1979

    Google Scholar 

  17. Lee, D. B. N., Brautbar, N., Walling, M. W., Silis, V., Carlson, H. E., Grindeland, R. E., Coburn, J. W., Kleeman, C. R.: The role of growth hormone in experimental phosphorus depletion, Calcif. Tissue Int.32:105, 1980

    CAS  PubMed  Google Scholar 

  18. Spanos, E.K., Colston, K. W., Evans, I. M. S., Galante, L. S., Macauley, S. I., MacIntyre, I.: Effect of prolactin on vitamin D metabolism, Mol. Cell Endocrinol.5:163, 1978

    Article  Google Scholar 

  19. Spanos, E., Pike, J. W., Haussler, M. R., Colston, K. W., Evans, I. M. A., Goldner, A. M., McCain, T. A., MacIntyre, I.: Circulating 1α,25-dihydroxyvitamin D in the chicken: enhancement by injection of prolactin and during egg laying, Life Sci.19:1751, 1976

    Article  CAS  PubMed  Google Scholar 

  20. Adams, N. D., Garthwaite, T. L., Gray, R. W., Hagen, T. C., Lemann, J. Jr.: The interrelationships among prolactin, 1,25-dihydroxyvitamin D, and parathyroid hormone in humans, J. Clin. Endocrinol. Metab.49:628–630, 1979

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Medicine and Biochemistry and the Clinical Research Center, Medical College of Wisconsin, 53226, Milwaukee, Wisconsin, USA

    Richard W. Gray

Authors
  1. Richard W. Gray
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gray, R.W. Control of plasma 1,25-(OH)2-vitamin D concentrations by calcium and phosphorus in the rat: Effects of hypophysectomy. Calcif Tissue Int 33, 485–488 (1981). https://doi.org/10.1007/BF02409478

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02409478

Key words

Search

Navigation