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Calcitonin (but not calcitonin gene-related peptide) increases mouse bone cell proliferation in a dose-dependent manner, and increases mouse bone formation, alone and in combination with fluoride

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Summary

Previousin vitro studies have shown that salmon calcitonin had direct effects to increase parameters associated with embryonic chicken bone formation and to increase mouse and chicken osteoblast-line cell proliferation. The current studies demonstrate increased cell proliferation (i.e., [3H]-thymidine incorporation into DNA and tetrazolium salt reduction/deposition) in the osteoblastic murine cell line MC-3T3-E1 in response to salmon calcitonin (P<0.005) and to human calcitonin (P<0.005), but not to human calcitonin gene-related peptide. The current studies also show that salmon calcitonin increased several indices of murine bone formation. We found that 72 hours of exposure to salmon calcitonin [at 5 mU/ml—about 0.37 nM; mU/ml = milliunits of calcitonin activity/ml incubation medium (at 4,000 U/mg protein)] increased net45Ca deposition (121% of control,P<0.05), net [3H]-proline incorporation 149% of control,P<0.001), and alkaline phosphatase activity (146% of control,P<0.01), in neonatal mouse half-calvaria. The calcitonin-dependent increase in alkaline phosphatase activity was not affected by co-incubation with 1 nM parathyroid hormone. Co-incubation with fluoride (which also increased net [3H]-proline incorporation and alkaline phosphatase activity in neonatal mouse half-calvaria,P<0.05, for each) enhanced the osteogenic response to low-dose calcitonin, (i.e., co-incubation with fluoride shifted the biphasic calcitonin dose-response curve to a range of lower calcitonin concentrations). The calcitonin-fluoride combinations had proportional effects on net [3H]-proline incorporation and alkaline phosphatase in the treated mouse calvaria (r=0.78,P<0.005).

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Authors and Affiliations

  1. Department of Medicine and Biochemistry, Loma Linda University, Loma Linda, California, USA

    John R. Farley, Susan L. Hall & Nanine M. Tarbaux

  2. Research Service-151, Jerry L. Pettis Memorial Veterans' Hospital, 11201 Benton Street, 92357, Loma Linda, CA, USA

    John R. Farley, Susan L. Hall & Nanine M. Tarbaux

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Farley, J.R., Hall, S.L. & Tarbaux, N.M. Calcitonin (but not calcitonin gene-related peptide) increases mouse bone cell proliferation in a dose-dependent manner, and increases mouse bone formation, alone and in combination with fluoride. Calcif Tissue Int 45, 214–221 (1989). https://doi.org/10.1007/BF02556040

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  • DOI: https://doi.org/10.1007/BF02556040

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