Abstract
This study investigates the molecular typing occurrence of staphylococcal communities during composting process of municipal solid waste. One hundred staphylococcal strains were isolated during the composting process and analysed either by phenotypic and genotypic methods. Amplified ribosomal DNA restriction analysis (ARDRA), internal transcripted spacer-polymerase chain reaction (ITS-PCR) and pulsed-field gel electrophoresis (PFGE) were used as molecular tools. During the digestion phase of composting process three staphylococcal species were recovered:Staphylococcus xylosus (48%),Staphylococcus lentus (40%), andStaphylococcus hominis (12%). In the maturation phase in addition to the latter species four species were also recovered at low frequencies:Staphylococcus lugdunensis, Staphylococcus haemolyticus, Staphylococcus warneri andStaphylococcus sciuri. Therefore, the composition of staphylococcal communities was temperature-, and phase dependant. The molecular methods showed that the ARDRA was not able to differentiate between strains of the same species. However, the ITS-PCR and the PFGE methods allowed interspecies and intraspecies discrimination, respectively.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ben Ayed L., Hassen A., Jedidi N., SaidiI N., Bouzaiane O., Murano F. (2007). Microbial C and N dynamics during composting process of urban solid waste. Waste Manage. Res., 25: 24–29.
Ben Kahla-Nakbi A., Besbes A., Bakhrouf A. (2008). Survival ofVibrio fluvialis in seawater under starvation conditions. Microbiol. Res., 163: 323–328.
Bes M., Guérin-Faublée V., Meugnier H., Etienne J., Freney J. (2000). Improvement of the identification of staphylococci isolated from bovine mammary infections using molecular methods. Vet. Microbiol., 71: 287–294.
Coppola S., Mauriello G., Aponte M., Moschetti G., Villani F. (2000). Microbial succession during ripening of Naples-type salami, a southern Italian fermented product. Meat Sci., 56: 321–329.
Freney J., Brun Y., Bes M., Meugnier H., Grimont F., Grimont PAD., Nervi C., Fleurette J. (1988).Staphylococcus lugdunensis sp. nov. andStaphylococcus schleiferi sp. nov. two species from human clinical specimens. Int. J. Syst. Bacteriol., 38: 168–172.
Gürtler V. (1993). Typing ofClostridium difficile strains by PCR-amplification of variable length 16S–23S rDNA spacer regions. J. Gen. Microbiol., 139: 3089–3097.
Hachicha R., Hassen A., Jedidi N., Kallali H. (1992). Optimal conditions for municipal solid waste. Biocycle, 6: 76–77.
Hachicha R., Jedidi N., Hassen A. (1993). Aspects hygiéniques de la fermentation aérobies des déchets urbains dans le contexte tunisien. Arch. Inst. Pasteur Tunis, 70: 13–20.
Hassen A., Belguith K., Jedidi N., Cherif A., Cherif M., Boudabbous A. (2001). Microbial characterization during composting of municipal solid waste. Bioresource Technol., 80: 185–192.
Hoppe-Seyler T., Jaeger B., Bockelmann W., Noordman W., Geis A., Heller AKJ. (2004). Molecular identification and differentiation ofStaphylococcus species and strains of cheese origin. Syst. Appl. Microbiol., 27: 211–218.
Jensen MA., Webster JA., Straus N. (1993). Rapid identification of bacteria on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms. Appl. Environ. Microbiol, 59: 945–952.
John JF., Gramling PK., O’Dell NM. (1992). Species identification of coagulase-negative staphylococci from urinary tract infcections. J. Clin. Microbiol., 8: 435–437.
Kamath U., Singer C., Isenberg HD. (1992). Clinical significance ofStaphylococcus warneri bacteremia. J. Clin. Microbiol., 30: 261–264.
Kloos W.E., Bannerman T.L. (1999).Staphylococcus andMicrococcus. In: Murray P.R., Baron E.J., Pfaller M.A., Tenover F.C., Yolken R.H., Eds, Manual of Clinical Microbiology, 7th edn, American Society for Microbiology, Washington, DC, pp. 264–282.
Lina G., Etienne J., Vandenesch F. (2000). Biology and pathogenicity of staphylococci other thanStaphylococcus aureus andS. epidermidis. In: Fischetti V.A, Novick R.P., Ferretti JJ., Portnoy D.A., Rood J.I., Eds, Gram-Positive Pathogens, American Society for Microbiology, Washington, DC, pp. 450–462.
Mendoza M., Meugnier H., Bes M., Etienne J., Freney J. (1998). Identification ofStaphylococcus species by 16S–23S rDNA intergenic spacer PCR analysis. Int. J. Syst. Bacteriol., 48: 1049–1055.
Marrie TJ., Kwan C., Noble MA., West A., Duffield L. (1982).Staphylococcus saprophyticus as a cause of urinary tract infections. J. Clin. Microbiol., 16: 427–431.
Martin M.A., Pfaller M.A., Wenzel R.P. (1989). Coagulase-negative staphylococcal bacteriemia. Ann. Intern. Med., 110: 9–16.
Marrug C., Grebus M., Hassen R.C., Keener H.M., Hoitink H.A.J. (1993). A kinetic model of yard waste composting process. Compost. Sci. Util., 1: 38–51.
Mustin M. (1987). Le Compost: Gestion de la Matière Organique. Editions François Dubusc, Paris.
Parente E., Martuscelli M., Gardini F., Grieco S., Crudele M.A., Suzzi G. (2001). Evolution of microbial populations and biogenic amine production in dry sausages produced in Southern Italy. J. Appl. Microbiol., 90: 882–891.
Pereira-Neto J.T., Stentiford E.I., Mara D.D. (1987). Low cost controlled composting of refuse and sewage sludge. In: De Bertoldi M, Ferranti M.P., L’Hermite P., Zucconi F., Eds, Compost: Production, Quality and Use, Elsevier Applied Science Publishers Ltd, London and New York.
Rossi F., Tofalo R., Torriani S., Suzzi G. (2001). Identification by 16S–23S rDNA intergenic region amplification, genotypic and phenotypic clustering ofStaphylococcus xylosus strains from dry sausages. J. Appl. Microbiol., 90: 365–371.
Sambrook J., Fritsch EF., Maniatis T. (1989). Molecular Cloning: A Laboratory Manual, 2nd edn., Cold Spring Harbor Press, New York.
Sikora L.J., Ramirez M., Troeschel T. (1983). Laboratory composure for simulation studies. J. Environ. Qual., 12: 219–224.
Straub T.M., Pepper I.L., Gerba C.P. (1993). Hazards from pathogenic microorganisms in land-disposed sewage sludge. Rev. Environ. Contam. T., 132: 55–91.
Strauch D. (1991). Survival of pathogenic micro-organism and parasites in exreta, manure and sewage sludge. Rev. Sci. Tech. Off. Int. Epiz., 10: 813–846.
Tammy L.B., Gary A.H., Fred C.T., Michael M.J. (1995). Pulsed-Field Gel Electrophoresis as a replacement for bacteriophage typing ofStaphylococcus aureus. J. Clin. Microbiol., 33: 551–555.
Tenover F.C., Arbeit R.D., Goering R.V., Mickelsen P.A., Murray B.E., Persing D.H., Swaminathan B. (1995). Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J. Clin. Microbiol., 33: 2233–2239.
Van Schaik W., Abee T. (2005). The role of δB in the stress response of Gram-positive bacteria-targets for food preservation and safety. Curr. Opin. Biotech., 16: 218–224.
Westblom T.U., Gorse G.J., Milligan T.W., Schindzielorz A.H. (1990). Anaerobic endocarditis caused byStaphylococcus saccharolyticus. J. Clin. Microbiol., 28: 2818–2819.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Bouzaiane, O., Abbassi, M.S., Gtari, M. et al. Molecular typing of staphylococcal communities isolated during municipal solid waste composting process. Ann. Microbiol. 58, 387–394 (2008). https://doi.org/10.1007/BF03175533
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF03175533