Abstract.
The increased incidence of obesity and related disorders in Western societies requires a thorough understanding of the adipogenic process. Data at the protein level of this process are scarce. Therefore we performed a proteome analysis of differentiating and starving 3T3-L1 cells using two-dimensional gel electrophoresis combined with mass spectrometry. Effects of different starvation conditions were examined by subjecting 3T3-L1 adipocytes to caloric restriction, either in the absence or the presence of the lipolysis inducer tumor necrosis factor-α. Ninety-three differentially expressed proteins were found during differentiation and starvation of 3T3-L1 cells, 50 of which were identified. GenMAPP/MAPP-finder software revealed a non-reciprocal regulation of the glycolytic pathway during 3T3-L1 differentiation followed by starvation. Furthermore, proteins involved in growth regulation, cytoskeletal rearrangements and protein modification, 16 of which have not been described before in 3T3-L1 cells, were identified. In conclusion, our data provide valuable information for further understanding of the adipogenic process.
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Received 9 November 2004; received after revision 21 December 2004; accepted 28 December 2004
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Renes, J., Bouwman, F., Noben, JP. et al. Protein profiling of 3T3-L1 adipocyte differentiation and (tumor necrosis factor α-mediated) starvation. CMLS, Cell. Mol. Life Sci. 62, 492–503 (2005). https://doi.org/10.1007/s00018-004-4498-9
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DOI: https://doi.org/10.1007/s00018-004-4498-9