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LC–MS/MS in the routine clinical laboratory: has its time come?

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Abstract

Liquid chromatography–tandem mass spectrometry (LC–MS/MS) has been increasingly used in routine clinical laboratories during the last two decades. The high specificity, sensitivity, and multi-analyte potential make it an ideal alternative to immunoassays or conventional high-performance liquid chromatography (HPLC). It also provides higher throughput than gas chromatography–mass spectrometry (GC–MS). LC–MS/MS also offers higher flexibility than immunoassays because LC–MS/MS assays are typically developed in-house. In addition, abundant information can be obtained from a single LC–MS/MS run which can produce a large amount of quantitative or qualitative data. In this review, typical LC–MS/MS clinical applications are presented, personal experiences are shared, and strengths and weakness are discussed. It is foreseeable that LC–MS/MS will become a key instrument in routine clinical laboratories.

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  1. Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Kowloon, HKSAR, China

    Kelvin Sze-Yin Leung & Bonnie Mei-Wah Fong

  2. Department of Pathology and Clinical Biochemistry, Queen Mary Hospital, Pokfulam Road, Pokfulam, HKSAR, China

    Bonnie Mei-Wah Fong

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  1. Kelvin Sze-Yin Leung
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Leung, K.SY., Fong, B.MW. LC–MS/MS in the routine clinical laboratory: has its time come?. Anal Bioanal Chem 406, 2289–2301 (2014). https://doi.org/10.1007/s00216-013-7542-5

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