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Antioxidant activity of sulphated polysaccharide conjugates from abalone (Haliotis discus hannai Ino)

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Abstract

The water-soluble sulphated polysaccharide conjugates were obtained from abalone viscera (Haliotis discus hannai Ino) by alkaline protease extraction followed by ethanol precipitation. Their antioxidant activities were evaluated in vitro by hydroxyl radicals scavenging activity, reducing power and metal chelating activity. Those various antioxidant activities were compared to standard antioxidants ascorbic acid and EDTA. The experimental results indicated that the crude extract having notable hydroxyl free radicals scavenging activity and moderate reducing power and chelating potency. The crude sulphated polysaccharide conjugates was enzymatically hydrolyzed by five commercially available proteases (trypsin, vernase, neutrase, pepsin and papain), and the resultant digests were tested for their antioxidant activities. Those proteolytic hydrolysates, although improving the hydroxyl radical scavenging activity in all cases except one, had lower reducing power and 3–15 times lower chelating ability than the native extract. Product derived from pepsin hydrolysate was fractionated by gel-filtration chromatography with sephadex G-100, giving two fractions containing sulphated polysaccharide conjugates termed ACP I and ACP II. The neutral monosaccharide composition of ACP I is rhamnose, fucose, xylose, mannose, galactose and glucose in a molar ratio of 1.00:45.14:4.00:5.36:33.18:2.15, with an average molecular weight of about 271 kDa. The neutral monosaccharide composition of ACP II is rhamnose, fucose, xylose, mannose, galactose and glucose in a molar ratio of 1.00:12.51:1.33:4.98:16.08:1.46, with an average molecular weight of about 6 kDa.

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Acknowledgements

This work was financially supported by Chinese International Corporation Project (No. 2006DFA32580), Innovational Team Project of the Educational Department of Liaoning Province (No. 2006T033) and The Project of Transformation of Research Findings of the Science and Technology Department of Liaoning Province (No. 2006301008).

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Authors and Affiliations

  1. College of Bio and Food Technology, Dalian Polytechnic University, Dalian, 116034, People’s Republic of China

    Bei-Wei Zhu, Li-Sha Wang, Da-Yong Zhou, Dong-Mei Li, Li-Ming Sun, Jing-Feng Yang & Xue-Qin Zhou

  2. Department of Biological Resources Chemistry, Faculty of Agriculture, Okayama University, Okayama, 700-8530, Japan

    Hai-Tao Wu & Mikiro Tada

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  1. Bei-Wei Zhu
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Correspondence to Bei-Wei Zhu.

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Zhu, BW., Wang, LS., Zhou, DY. et al. Antioxidant activity of sulphated polysaccharide conjugates from abalone (Haliotis discus hannai Ino). Eur Food Res Technol 227, 1663–1668 (2008). https://doi.org/10.1007/s00217-008-0890-2

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