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Phenotype and expression profile analysis of Staphylococcus aureus biofilms and planktonic cells in response to licochalcone A

  • Applied microbial and cell physiology
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Abstract

Staphylococcus aureus is one of the most important pathogens in humans and animals. The formation of biofilm by S. aureus is considered an important mechanism of antimicrobial resistance. Therefore, finding effective drugs against the biofilm produced by S. aureus has been a high priority. Licochalcone A (LAA), a natural plant product, was reported to have antibacterial activities and showed good activity against all 21 tested strains of S. aureus biofilm and planktonic cells. To detect the possible molecular mechanism of LAA against S. aureus biofilm or planktonic cells, Affymetrix GeneChips were used to determine the global comparative transcription of S. aureus biofilm and planktonic cells triggered by treatment with sub-bactericidal and sub-inhibitory concentrations of LAA, respectively. LAA significantly altered (greater than a 2- or less than −2-fold change) the expression of 693 genes in planktonic cells and 817 genes in biofilm. The levels of genes encoding autolysis-associated proteins, cell wall proteins, pathogenic factors, protein synthesis genes, and enzymes involved in capsule synthesis were significantly altered in LAA-treated S. aureus. Furthermore, some differences observed in the microarray analysis were verified by real-time RT–PCR. To our knowledge, this is the first observation of phenotype and expression profiles of S. aureus biofilm and planktonic cells in response to LAA treatment.

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Acknowledgments

This work was supported by Important National Science and Technology Specific Projects (2012ZX10003002), the National Nature Science Foundation of China (No. 31172364; No. 31271951; No. 31000822), Program for New Century Excellent Talents in University (NCET-09-0434; NCET-13-0245), Fundamental Research Program of Shen Zhen (JCYJ20130401172016183; JCYJ20120616142424467), and Shenzhen Promotion Plan Basic Research Laboratory in 2012 (ZDSY20120616141302982).

Conflict of interest

The authors declare no conflicts of interest.

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Authors and Affiliations

  1. Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Science and Department of Food Quality and Safety, Jilin University, Changchun, China

    Fengge Shen, Zhiqiang Yang, Xiaochen Shi, Chao Wang, Qiaoli Zhang, Yanan An, Wei Cheng, Kunqi Jin, Mingyuan Liu, Na Guo & Lu Yu

  2. Key Lab for New Drug Research of TCM, Research Institute of Tsinghua University in Shenzhen, Shenzhen, China

    Xudong Tang

  3. Department of Infectious Diseases, First Hospital of Jilin University, Changchun, China

    Yang Wang

  4. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China

    Mingyuan Liu

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  1. Fengge Shen
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  2. Xudong Tang
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  3. Yang Wang
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  4. Zhiqiang Yang
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  5. Xiaochen Shi
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  8. Yanan An
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  9. Wei Cheng
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  10. Kunqi Jin
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  11. Mingyuan Liu
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  12. Na Guo
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  13. Lu Yu
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Corresponding authors

Correspondence to Na Guo or Lu Yu.

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Fengge Shen and Xudong Tang contributed equally to this work.

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Shen, F., Tang, X., Wang, Y. et al. Phenotype and expression profile analysis of Staphylococcus aureus biofilms and planktonic cells in response to licochalcone A. Appl Microbiol Biotechnol 99, 359–373 (2015). https://doi.org/10.1007/s00253-014-6076-x

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  • DOI: https://doi.org/10.1007/s00253-014-6076-x

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