Abstract
In 2005, an epidemic of the cestode Triaenophorus crassus in Arctic charr, Salvelinus umbla from Lake Grundlsee (Austria) was reported, resulting in the closure of the local fisheries. The parasite uses a copepod and a salmonid fish as intermediate hosts, and the European pike (Esox lucius) as definitive host. In Lake Grundlsee procercoids of T. crassus, T. nodulosus and Eubothrium salvelini use the copepod Cyclops abyssorum praealpinus as their first intermediate host. We report on the development of a PCR–RFLP assay for the specific differentiation between the procercoids of the three cestode species. Ban I restriction sites within a 207-bp 18S rRNA-amplified fragment yielded two and three species-specific products to unequivocally distinguish between T. crassus and T. nodulosus, respectively. The assay is sufficiently sensitive to characterise a single procercoid of both Triaenophorus species in their copepod host, enabling a direct measure of infection. This assay will be useful in monitoring the progress of the control methods currently in place.
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References
Achleitner D, Gassner H, Schabetsberger R (2009) ‘Global worming’: first record of an epidemic of Triaenophorus crassus in a population of Arctic charr Salvelinus umbla. J Fish Biol 74:961–966
Gassner H, Wanzenböck J, Zick D, Tischler G, Pamminger-Lahnsteiner B (2005) Development of a fish based lake typology for natural Austrian Lakes >50 ha based on the reconstructed historical fish communities. Int Rev Hydrobiol 90:422–432
Hoffman RW, Meder J, Klein M, Osterkornj K, Negele RD (1986) Studies on lesions caused by plerocercoids of Triaenophorus nodulosus in some fish of an alpine lake, the Königssee. J Fish Biol 28:701–712
Kuperman BI (1981) Tapeworms of the genus Triaenophorus parasites of fishes. Amerind Publications, New Dehli
Lahnsteiner F, Kletzl M, Weismann T (2009) The risk of parasite transfer to juvenile fishes by live copepod food with the example Triaenophorus crassus and Triaenophorus nodulosus. Aquaculture 295:120–125
Pulkkinen K, Valtonen ET (1999) Accumulation of plerocercoids of Triaenophorus crassus in the second intermediate host Coregonus lavaretus and their effect on growth of the host. J Fish Biol 55:115–126
Rosen R, Dick TA (1984) Experimental infections of rainbow trout Salmo gairdneri Richardson, with plerocercoids of Triaenophorus crassus Forel. J Wildl Dis 20:34–38
Rydlo M (1993) Parasitische Helminten in Aalrutten (Lota lota L.) aus Seen und Fliessgewässern Österreichs. Ann Naturhist Mus Wien 94(95):41–45
Rydlo M (1998) Synopsis der Zooparasiten der Fische Österreichs – Endohelminthen in Fischen aus Seen und Fliessgewässern Westösterreichs. Mitt Österr Ges Tropenmed Parasitol 20:109–116
Schäperclaus W, Kulow H, Schreckenbach K (1990) Fischkrankheiten. Verlag, Berlin
Wallner J (1911) Beiträge zur Geschichte des Fischereiwesens in der Steiermark. In: Forschungen zur Verwaltungs-und Verfassungsgeschichte der Steiermark 8:1–134
Williams H, Jones A (1994) Parasitic worms of fish. Taylor & Francis Ltd., London
Acknowledgements
We wish to thank C. Stögner and G. Schaufler for help in the field. This project was funded through a joint project of the Austrian Academy of Sciences, the Austrian Ministry of Agriculture, the Provincial Government of Styria, and the Federal Forest Department.
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Boufana, B., Žibrat, U., Jehle, R. et al. Differential diagnosis of Triaenophorus crassus and T. nodulosus experimental infection in Cyclops abyssorum praealpinus (Copepoda) from the Alpine Lake Grundlsee (Austria) using PCR–RFLP. Parasitol Res 109, 745–750 (2011). https://doi.org/10.1007/s00436-011-2317-4
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DOI: https://doi.org/10.1007/s00436-011-2317-4