Abstract
Basic fibroblast growth factor (bFGF) is a cytokine that promotes the regeneration of the periodontium, the specialized tissues supporting the teeth. bFGF, does not, however, induce the synthesis of smooth muscle actin alpha 2 (ACTA2), type I collagen (COL1), or COL3, which are principal molecules in periodontal ligament (PDL) tissue, a component of the periodontium. We have suggested the feasibility of using transforming growth factor-β1 (TGFβ1) to induce fibroblastic differentiation of PDL stem/progenitor cells (PDLSCs). Here, we investigated the effect of the subsequent application of TGFβ1 after bFGF (bFGF/TGFβ1) on the differentiation of PDLSCs into fibroblastic cells. We first confirmed the expression of bFGF and TGFβ1 in rat PDL tissue and primary human PDL cells. Receptors for both bFGF and TGFβ1 were expressed in the human PDLSC lines 1-11 and 1-17. Exposure to bFGF for 2 days promoted vascular endothelial growth factor gene and protein expression in both cell lines and down-regulated the expression of ACTA2, COL1, and COL3 mRNA in both cell lines and the gene fibrillin 1 (FBN1) in cell line 1-11 alone. Furthermore, bFGF stimulated cell proliferation of these cell lines and significantly increased the number of cells in phase G2/M in the cell lines. Exposure to TGFβ1 for 2 days induced gene expression of ACTA2 and COL1 in both cell lines and FBN1 in cell line 1-11 alone. BFGF/TGFβ1 treatment significantly up-regulated ACTA2, COL1, and FBN1 expression as compared with the group treated with bFGF alone or the untreated control. This method might thus be useful for accelerating the generation and regeneration of functional periodontium.
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Abbreviations
- ACTA2:
-
Smooth muscle actin alpha 2
- bFGF:
-
Basic fibroblast growth factor
- COL1:
-
Type I collagen
- COL3:
-
Type III collagen
- DAPI:
-
4,6-Diamidino-2-phenylindole
- FBN1:
-
Fibrillin 1
- FGFR:
-
Fibroblast growth factor receptor
- HPDLC:
-
Human periodontal ligament cell
- PDL:
-
Periodontal ligament
- PDLSC:
-
Periodontal ligament stem/progenitor cell
- TGFβ1:
-
Transforming growth factor-β1
- TGFBR:
-
Transforming growth factor-β receptor
- VEGF:
-
Vascular endothelial growth factor
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Acknowledgments
We thank Drs. Hasegawa and Yuda for their great support in the preparation of this work.
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This work was financially supported by Grants-in-Aid for Scientific Research (Project nos. 22390359, 23659890, 23689077, 24390426, 24659848, and 24792028) from the Japan Society for the Promotion of Science.
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Kono, K., Maeda, H., Fujii, S. et al. Exposure to transforming growth factor-β1 after basic fibroblast growth factor promotes the fibroblastic differentiation of human periodontal ligament stem/progenitor cell lines. Cell Tissue Res 352, 249–263 (2013). https://doi.org/10.1007/s00441-012-1543-0
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DOI: https://doi.org/10.1007/s00441-012-1543-0