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Manganese-doped carbon quantum dots for fluorometric and magnetic resonance (dual mode) bioimaging and biosensing

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Abstract

Manganese-doped carbon quantum dots (MnCQDs) were prepared through one-step hydrothermal method using citric acid and manganese tetraphenyl porphyrin as carbon sources in aqueous media. The structure of MnCQDs was confirmed by TEM, XRD, and XPS. The MnCQDs display a typical excitation-dependent emission behavior and exhibit bright green luminescence (with a peak at 482 nm) under UV irradiation (365 nm) and a fluorescence quantum yield of 13%. The MnCQDs can be used as a fluorescent probe for ferric ion in aqueous solution with a 220 nM detection limit. The MTT assay demonstrated the low cytotoxicity of MnCQDs towards HeLa cells. Due to the excitation-dependent emission properties, MnCQDs can be used as a multi-color (blue, green, and red) bioimaging agent in cancer cells and in living zebrafish. The application of MnCQDs as selective biosensing probe for Fe3+ was also realized in cells and zebrafish mode. Because of the existence of paramagnetic ions, MnCQDs demonstrate an enhanced magnetic resonance (MR) signal. Thus, the MnCQDs can serve as a positive contrast agent for MR imaging.

Schematic presentation of the preparation of luminescent manganese-doped carbon quantum dots (MnCQDs). MnCQDs showed good magnetic resonance effect and can be used as a fluorescence probe for the detection of Fe3+ in HeLa cells and zebrafish.

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Acknowledgments

We are thankful for the support from National Natural Science Foundation of China (grant no. 21601142, 21804102), Natural Science Foundation of Hubei Province (grant no. 2018CFB159, 2017CFB222) and the 10th Graduate Innovative Fund of Wuhan Institute of Technology (CX2018005).

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Authors and Affiliations

  1. Key Laboratory for Green Chemical Process of the Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan, 430205, People’s Republic of China

    Liangliang Yue, Haolan Li, Qi Liu, Ding Guo, Yingzhi Xu & Fengshou Wu

  2. School of Chemistry and Environmental Engineering, Wuhan Institute of Technology, Wuhan, 430205, People’s Republic of China

    Jun Chen & Qi Sun

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  1. Liangliang Yue
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Correspondence to Qi Sun or Fengshou Wu.

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Yue, L., Li, H., Liu, Q. et al. Manganese-doped carbon quantum dots for fluorometric and magnetic resonance (dual mode) bioimaging and biosensing. Microchim Acta 186, 315 (2019). https://doi.org/10.1007/s00604-019-3407-8

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