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Mitochondrial VDAC and hexokinase together modulate plant programmed cell death

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Abstract

The voltage-dependent anion channel (VDAC) and mitochondrially located hexokinase have been implicated both in pathways leading to cell death on the one hand, and immortalization in tumor formation on the other. While both proteins have also been implicated in death processes in plants, their interaction has not been explored. We have examined cell death following heterologous expression of a rice VDAC in the tobacco cell line BY2 and in leaves of tobacco plants and show that it is ameliorated by co-expression of hexokinase. Hexokinase also abrogates death induced by H2O2. We conclude that the ratio of expression of the two proteins and their interaction play a major role in modulating death pathways in plants.

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Acknowledgements

We thank Prof. Usha VijayRaghavan (Indian Institute of Science, Bangalore, India) for gifting us with OsVDAC4 cDNA. The gift of pBin-NtHxK3-GFP construct from Prof. Uwe Sonnewald is gratefully acknowledged. We thank Dr. Joachim Forner (Molekulare Botanik, Universität Ulm, Germany) for kind gift of eqFP611-pUC19 plasmid. We thank Mr. Anirban Baral for timely help at several instances during the course of the study. AG thanks the Department of Biotechnology for a postdoctoral fellowship. AKD thanks the Indian Council of Medical Research for Senior Research Fellowship. We acknowledge the support of Central Imaging and Flow Facility at NCBS for imaging. This work was supported by internal funds from NCBS.

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Author notes

  1. Ashwini Godbole

    Present address: Centre for Pharmacognosy, Pharmaceutics and Pharmacology, Institute of Ayurveda and Integrative Medicine, Bangalore, 560064, India

  2. Palakolanu S. Reddy

    Present address: Department of Biotechnology, School of Life Science, University of Hyderabad, Hyderabad, 500046, India

Authors and Affiliations

  1. National Centre for Biological Sciences, TIFR,UAS-GKVK Campus, Bangalore, 560065, India

    Ashwini Godbole, Ashvini Kumar Dubey, Palakolanu S. Reddy & Mathew K. Mathew

  2. Department of Crop Physiology, GKVK, University of Agricultural Sciences, Bangalore, 560065, India

    Ashwini Godbole & M. Udayakumar

  3. Department of Biotechnology, University of Mysore, Mysore, 570006, India

    Ashvini Kumar Dubey

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  1. Ashwini Godbole
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Correspondence to Mathew K. Mathew.

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Handling Editor: Jaideep Mathur

Ashwini Godbole and Ashvini Kumar Dubey authors contributed equally to this research

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Godbole, A., Dubey, A.K., Reddy, P.S. et al. Mitochondrial VDAC and hexokinase together modulate plant programmed cell death. Protoplasma 250, 875–884 (2013). https://doi.org/10.1007/s00709-012-0470-y

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