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Fabrication of a Novel Absorbable Vascular Anastomosis Device and Testing in a Pig Liver Transplantation Model

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Abstract

An absorbable vascular anastomosis device (AVAD) was fabricated and tested in pig liver transplantation experiments. We prepared biodegradable copolymers with various ratios of l-lactide: glycolide and tested their properties including inherent viscosity, in vitro biodegradation, and tensile strength. The mechanical and physical properties of the finally selected copolymers were analyzed according to decomposition time. The AVAD, consisting of two inner rings and one coupler, was fabricated with 5-, 15-, and 20-mm diameter sizes using an injection molding machine. Based on changes in the overall mechanical and physical properties, it is believed that the AVAD will maintain its shape without deformation while connecting the blood vessels to external force for at least 3 weeks. Four mini-pigs underwent liver transplantation with AVAD using livers obtained from swine leukocyte antigen–identical siblings. Anastomoses was achieved in all four cases. In case 4, an autopsy conducted at 4 months revealed that the AVAD was absorbed and the anastomosis was intact, demonstrating the success of the AVAD in the pig liver transplantation experiments and the feasibility of using an AVAD in organ transplantation.

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Abbreviations

AVAD:

Absorbable vascular anastomotic device

AFM:

Atomic force microscopy

ASTM D638:

Standard test method for tensile properties of plastics

DSC:

Differential scanning calorimetry

NMR:

Nuclear magnetic resonance

PLA:

Poly(lactic acid)

PLGA:

Poly(lactide-co-glycolide)

PGA:

Poly(glycolic acid)

SLA:

Swine leukocyte antigen

TGA:

Thermal gravimetric analysis

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Acknowledgments

This work was supported by the Beyond Leading Technology R&D Program of the Small and Medium Business Administration [S20146915, 2014].

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Authors and Affiliations

  1. Division of Transplant and Vascular Surgery, Department of Surgery, Keimyung University School of Medicine, Daegu, Korea

    Ui Jun Park & Hyoung Tae Kim

  2. Department of Plastic and Reconstructive Surgery, Keimyung University School of Medicine, Daegu, Korea

    Woonhyeok Jeong & Daegu Son

  3. Department of Pathology, Keimyung University, Daegu, Korea

    Sun Young Kwon

  4. Metabiomed Co. Ltd., Osong, Korea

    Yunkun Kim

  5. Optipharm Co. Ltd., Osong, Korea

    Kimyung Choi

  6. Department of Plastic and Reconstructive Surgery, School of Medicine and Institute for Medical Science, Keimyung University, 56 Dalseong-ro, Jung-gu, Daegu, 41931, South Korea

    Daegu Son

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  1. Ui Jun Park
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  2. Woonhyeok Jeong
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  6. Hyoung Tae Kim
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  7. Daegu Son
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Correspondence to Daegu Son.

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Associate Editor Elena S. Di Martino oversaw the review of this article.

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Park, U.J., Jeong, W., Kwon, S.Y. et al. Fabrication of a Novel Absorbable Vascular Anastomosis Device and Testing in a Pig Liver Transplantation Model. Ann Biomed Eng 47, 1063–1077 (2019). https://doi.org/10.1007/s10439-019-02212-5

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  • DOI: https://doi.org/10.1007/s10439-019-02212-5

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