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Association among 1267 A/G HSP70-2, −308 G/A TNF-α polymorphisms and pro-inflammatory plasma mediators in old ZincAge population

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Abstract

Proinflammatory cytokines and heat shock proteins play relevant roles in the pathogenesis of inflammatory diseases. We investigated whether Hsp70 1267 A/G and TNF-α −308 G/A polymorphisms are associated with proinflammatory mediators, zinc status and laboratory parameters in 1,078 healthy elderly from ZincAge study. Hsp70 1267 A/G genotype and allele distribution were similar among various European countries, while a TNF-α genetic heterogeneity was observed between the Northern and the Southern European populations, with a major frequency of the −308 A variant in France, Germany and Poland. We used linear regression models to test additive, dominant or recessive associations of each SNP with proinflammatory mediators, laboratory parameters, metallothioneins and zinc status. Hsp70 1267 A/G SNP, but not TNF-α −308 G/A SNP, influences TNF-α and IL-6 plasma levels under additive, dominant and recessive models (for TNF-α only). An association between Hsp70 1267 A/G SNP and zinc plasma levels was observed in the dominant model. In particular, G allele carriers showed increased circulating pro-inflammatory cytokines and zinc. Moreover, both these SNPs affect creatinine levels suggesting a possible influence on renal function. In conclusion, Hsp70 1267 A/G SNP is associated with pro-inflammatory cytokine production in healthy elderly and might represent a possible determinant of individual susceptibility to inflammatory diseases.

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Acknowledgments

This study was supported by INRCA and European Commission (Project ZincAge: FOOD-CT-2003-506850; E. Mocchegiani, Coordinator).

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Authors and Affiliations

  1. Translational Research Ctr. of Nutrition and Ageing, IRCCS-INRCA, Via Birarelli 8, 60121, Ancona, Italy

    R. Giacconi, L. Costarelli, M. Malavolta, F. Piacenza, N. Gasparini, A. Basso & E. Mocchegiani

  2. Clinical Laboratory & Molecular Diagnostics, INRCA-IRCCS, Ancona, Italy

    R. Galeazzi & F. Busco

  3. Laboratory of Immunorheumatology and Tissue Regeneration/RAMSES, Rizzoli Orthopedic Institute, Bologna, Italy

    E. Mariani

  4. Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy

    E. Mariani

  5. Research Center on Aging, Department of Medicine, Faculty of Medicine, University of Sherbrooke, Sherbrooke, QC, Canada

    T. Fulop

  6. Institute of Immunology, Medical Faculty, RWTH Aachen University, Aachen, Germany

    L. Rink

  7. Department of Dietetics and Nutritional Science, Harokopio University of Athens, Athens, Greece

    G. Dedoussis & S. Kanoni

  8. Department of Virology, Franche-Comte University, Besançon, France

    G. Herbein

  9. Department of Toxicology, Faculty of Pharmacy, Medical University, Lodz, Poland

    J. Jajte

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  1. R. Giacconi
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  2. L. Costarelli
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  3. M. Malavolta
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  9. T. Fulop
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  10. L. Rink
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  11. G. Dedoussis
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  12. S. Kanoni
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  13. G. Herbein
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  15. F. Busco
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  16. E. Mocchegiani
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Correspondence to R. Giacconi.

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Giacconi, R., Costarelli, L., Malavolta, M. et al. Association among 1267 A/G HSP70-2, −308 G/A TNF-α polymorphisms and pro-inflammatory plasma mediators in old ZincAge population. Biogerontology 15, 65–79 (2014). https://doi.org/10.1007/s10522-013-9480-1

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